{"_id":"9NsyQJsXu8QkburuL","bibbaseid":"anonymous-plasmaexosomesstimulatebreastcancermetastasisthroughsurfaceinteractionsandactivationoffaksignaling-2019","authorIDs":[],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Shtam"],"firstnames":["T."],"suffixes":[]},{"propositions":[],"lastnames":["Naryzhny"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Samsonov"],"firstnames":["R."],"suffixes":[]},{"propositions":[],"lastnames":["Karasik"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Mizgirev"],"firstnames":["I."],"suffixes":[]},{"propositions":[],"lastnames":["Kopylov"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Petrenko"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Zabrodskaya"],"firstnames":["Y."],"suffixes":[]},{"propositions":[],"lastnames":["Kamyshinsky"],"firstnames":["R."],"suffixes":[]},{"propositions":[],"lastnames":["Nikitin"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Sorokin"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Buzdin"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Gil-Henn"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Malek"],"firstnames":["A."],"suffixes":[]}],"title":"Plasma exosomes stimulate breast cancer metastasis through surface interactions and activation of FAK signaling","journal":"Breast Cancer Research and Treatment","year":"2019","volume":"174","number":"1","pages":"129-141","doi":"10.1007/s10549-018-5043-0","note":"cited By 5","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057307490&doi=10.1007%2fs10549-018-5043-0&partnerID=40&md5=7dc63693ded805a90ebd2bcc5dbf25bb","affiliation":"N.N. Petrov National Medical Research Center of Oncology, Leningradskaya 68, St.-Petersburg, 197758, Russian Federation; Oncosystem Ltd., Lugovaya 4, Skolkovo Innovation Center, Moscow, 143026, Russian Federation; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Center “Kurchatov Institute”, Orlova roscha 1, Gatchina, 188300, Russian Federation; Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russian Federation; The Azrieli Faculty of Medicine, Bar-Ilan University, Henrietta Szold 8, Safed, 1311502, Israel; Smorodintsev Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, prof. Popov str. 15/17, St.-Petersburg, 197376, Russian Federation; National Research Center “Kurchatov Institute”, Akademika Kurchatova pl., 1, Moscow, 123182, Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation; Omics Way Corp., Walnut, CA, United States; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow, 119991, Russian Federation","abstract":"Purpose: The interaction between malignant cells and surrounding healthy tissues is a critical factor in the metastatic progression of breast cancer (BC). Extracellular vesicles, especially exosomes, are known to be involved in inter-cellular communication during cancer progression. In the study presented herein, we aimed to evaluate the role of circulating plasma exosomes in the metastatic dissemination of BC and to investigate the underlying molecular mechanisms of this phenomenon. Methods: Exosomes isolated from plasma of healthy female donors were applied in various concentrations into the medium of MDA-MB-231 and MCF-7 cell lines. Motility and invasive properties of BC cells were examined by random migration and Transwell invasion assays, and the effect of plasma exosomes on the metastatic dissemination of BC cells was demonstrated in an in vivo zebrafish model. To reveal the molecular mechanism of interaction between plasma exosomes and BC cells, a comparison between un-treated and enzymatically modified exosomes was performed, followed by mass spectrometry, gene ontology, and pathway analysis. Results: Plasma exosomes stimulated the adhesive properties, two-dimensional random migration, and transwell invasion of BC cells in vitro as well as their in vivo metastatic dissemination in a dose-dependent manner. This stimulatory effect was mediated by interactions of surface exosome proteins with BC cells and consequent activation of focal adhesion kinase (FAK) signaling in the tumor cells. Conclusions: Plasma exosomes have a potency to stimulate the metastasis-promoting properties of BC cells. This pro-metastatic property of normal plasma exosomes may have impact on the course of the disease and on its prognosis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.","author_keywords":"Breast cancer; Exosomes; FAK signaling; Mass spectrometry; Metastasis; Surface interaction","funding_details":"Israel Cancer Association20180089","key":"Shtam2019129","id":"Shtam2019129","bibbaseid":"anonymous-plasmaexosomesstimulatebreastcancermetastasisthroughsurfaceinteractionsandactivationoffaksignaling-2019","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057307490&doi=10.1007%2fs10549-018-5043-0&partnerID=40&md5=7dc63693ded805a90ebd2bcc5dbf25bb"},"metadata":{"authorlinks":{}},"downloads":2},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lpro_2019_10.txt","creationDate":"2019-11-05T13:18:15.225Z","downloads":2,"keywords":[],"search_terms":["plasma","exosomes","stimulate","breast","cancer","metastasis","through","surface","interactions","activation","fak","signaling"],"title":"Plasma exosomes stimulate breast cancer metastasis through surface interactions and activation of FAK signaling","year":2019,"dataSources":["TRehu3DvnmfePetpJ","YYLjupmCazkNqWNEJ","eoAALb5LHAEShrPHa"]}