{"_id":"8Hx7Tdx2HT5fqaMD6","bibbaseid":"anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003","authorIDs":[],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Soboleva"],"firstnames":["N.G."],"suffixes":[]},{"propositions":[],"lastnames":["Makhno"],"firstnames":["V.I."],"suffixes":[]},{"propositions":[],"lastnames":["Konevega"],"firstnames":["A.L."],"suffixes":[]},{"propositions":[],"lastnames":["Semenkov"],"firstnames":["Yu.P."],"suffixes":[]},{"propositions":[],"lastnames":["Katunin"],"firstnames":["V.I."],"suffixes":[]}],"title":"The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the a Site of the 70S Ribosome","journal":"Molecular Biology","year":"2003","volume":"37","number":"1","pages":"110-115","doi":"10.1023/A:1022345115927","note":"cited By 0","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&doi=10.1023%2fA%3a1022345115927&partnerID=40&md5=741954e32b6b93a44fe9c09521d9d1d0","affiliation":"Konstantinov Inst. of Nucl. Physics, Russian Academy of Sciences, Gatchina, Leningrad Region, 188300, Russian Federation","abstract":"To estimate the effect of modified nucleotide 37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA+YPhe and Phe-tRN A -YPhe) with the A site of complex [70S·poly(U) ·deacylated tRNAPhe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA+YPhe showed, removal of the Y base decreased the association constant of Phe-tRN A-Y Phe and the complex by an order of magnitude at every temperature tested, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNAPhe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRN A-YPhe but not for Phe-tRN A +YPhe. Thus, the modified nucleotide 3′ of the Phe-tRNAPhe anticodon stabilized the codon-anticodon interaction both in the A and P sites of the 70S ribosome.","author_keywords":"70S ribosome; A site; Modified nucleotide; tRNAPhe; Y-base","funding_details":"Российский Фонд Фундаментальных Исследований (РФФИ)01-04-49252","key":"Soboleva2003110","id":"Soboleva2003110","bibbaseid":"anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&doi=10.1023%2fA%3a1022345115927&partnerID=40&md5=741954e32b6b93a44fe9c09521d9d1d0"},"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lkb_2019_10.txt","creationDate":"2019-11-05T13:48:15.650Z","downloads":0,"keywords":[],"search_terms":["effect","modification","nucleotide","interaction","aminoacyl","trna","site","70s","ribosome"],"title":"The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the a Site of the 70S Ribosome","year":2003,"dataSources":["TRehu3DvnmfePetpJ","cJ5ZpoJMJycEnWRby"]}