Sequence Effects of Single Base Loops in Intramolecular Quadruplex DNA. a Rachwal, P., Brown, T., & Fox, K. R FEBS letters, 581(8):1657–60, May, 2007. doi abstract bibtex We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.
@article{Rachwal2007,
title = {Sequence Effects of Single Base Loops in Intramolecular Quadruplex {{DNA}}.},
author = {a Rachwal, Phillip and Brown, Tom and Fox, Keith R},
year = {2007},
month = may,
journal = {FEBS letters},
volume = {581},
number = {8},
eprint = {17399710},
eprinttype = {pubmed},
pages = {1657--60},
issn = {0014-5793},
doi = {10.1016/j.febslet.2007.03.040},
abstract = {We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.},
pmid = {17399710},
keywords = {\#nosource,Base Sequence,Deoxyribose,Deoxyribose: analysis,DNA,DNA: chemistry,DNA: genetics,G-Quadruplexes,Nucleic Acid Conformation,Oligonucleotides,Oligonucleotides: chemistry,Oligonucleotides: genetics,Thermodynamics}
}
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