@article {Araujo655803,
	author = {Jackeline S Araujo and Rui M P Silva-Junior and Tong Zhang and Cara R Schiavon and Qian Chu and Melissa Wu and Carmen L S Pontes and Anderson O Souza and Luciane C Alberici and Aline M Santos and Sadie Bartholomew Ingle and Alan Saghatelian and James Spudich and Uri Manor and Enilza M Espreafico},
	title = {A novel role for Myosin-Va in mitochondrial fission},
	elocation-id = {655803},
	year = {2019},
	doi = {10.1101/655803},
	publisher = {Cold Spring Harbor Laboratory},
	abstract = {In cancer cells metabolic changes and mitochondrial morphology are coupled. It is known that the cytoskeleton and molecular motors are directly involved in regulating mitochondrial morphology. Here we show that myosin-Va, an actin-based molecular motor, is required for the malignant properties of melanoma cells and localizes to mitochondria in these cells. Knockdown of myosin-Va increases cellular respiration rates and ROS production and decreases glucose uptake and lactate secretion. In addition, knockdown of myosin-Va results in reduced mitochondrial fission and correspondingly elongated mitochondria. We show that myosin-Va interacts with the mitochondrial outer membrane protein Spire1C, an actin-regulatory protein implicated in mitochondrial fission, and that Spire1C recruits myosin-Va to mitochondria. Finally, we show that during mitochondrial fission myosin-Va localization to mitochondria increases, and that myosin-Va localizes to mitochondrial fission sites immediately adjacent to Drp1 punctae. We conclude that myosin-Va facilitates mitochondrial fission. These data implicate myosin-Va as a target for the Warburg effect in melanoma cells.},
	URL = {https://www.biorxiv.org/content/early/2019/05/31/655803},
	eprint = {https://www.biorxiv.org/content/early/2019/05/31/655803.full.pdf},
	journal = {bioRxiv}
}

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M"],"bibdata":{"bibtype":"article","type":"article","author":[{"firstnames":["Jackeline","S"],"propositions":[],"lastnames":["Araujo"],"suffixes":[]},{"firstnames":["Rui","M","P"],"propositions":[],"lastnames":["Silva-Junior"],"suffixes":[]},{"firstnames":["Tong"],"propositions":[],"lastnames":["Zhang"],"suffixes":[]},{"firstnames":["Cara","R"],"propositions":[],"lastnames":["Schiavon"],"suffixes":[]},{"firstnames":["Qian"],"propositions":[],"lastnames":["Chu"],"suffixes":[]},{"firstnames":["Melissa"],"propositions":[],"lastnames":["Wu"],"suffixes":[]},{"firstnames":["Carmen","L","S"],"propositions":[],"lastnames":["Pontes"],"suffixes":[]},{"firstnames":["Anderson","O"],"propositions":[],"lastnames":["Souza"],"suffixes":[]},{"firstnames":["Luciane","C"],"propositions":[],"lastnames":["Alberici"],"suffixes":[]},{"firstnames":["Aline","M"],"propositions":[],"lastnames":["Santos"],"suffixes":[]},{"firstnames":["Sadie","Bartholomew"],"propositions":[],"lastnames":["Ingle"],"suffixes":[]},{"firstnames":["Alan"],"propositions":[],"lastnames":["Saghatelian"],"suffixes":[]},{"firstnames":["James"],"propositions":[],"lastnames":["Spudich"],"suffixes":[]},{"firstnames":["Uri"],"propositions":[],"lastnames":["Manor"],"suffixes":[]},{"firstnames":["Enilza","M"],"propositions":[],"lastnames":["Espreafico"],"suffixes":[]}],"title":"A novel role for Myosin-Va in mitochondrial fission","elocation-id":"655803","year":"2019","doi":"10.1101/655803","publisher":"Cold Spring Harbor Laboratory","abstract":"In cancer cells metabolic changes and mitochondrial morphology are coupled. It is known that the cytoskeleton and molecular motors are directly involved in regulating mitochondrial morphology. Here we show that myosin-Va, an actin-based molecular motor, is required for the malignant properties of melanoma cells and localizes to mitochondria in these cells. Knockdown of myosin-Va increases cellular respiration rates and ROS production and decreases glucose uptake and lactate secretion. In addition, knockdown of myosin-Va results in reduced mitochondrial fission and correspondingly elongated mitochondria. We show that myosin-Va interacts with the mitochondrial outer membrane protein Spire1C, an actin-regulatory protein implicated in mitochondrial fission, and that Spire1C recruits myosin-Va to mitochondria. Finally, we show that during mitochondrial fission myosin-Va localization to mitochondria increases, and that myosin-Va localizes to mitochondrial fission sites immediately adjacent to Drp1 punctae. We conclude that myosin-Va facilitates mitochondrial fission. These data implicate myosin-Va as a target for the Warburg effect in melanoma cells.","url":"https://www.biorxiv.org/content/early/2019/05/31/655803","eprint":"https://www.biorxiv.org/content/early/2019/05/31/655803.full.pdf","journal":"bioRxiv","bibtex":"@article {Araujo655803,\r\n\tauthor = {Jackeline S Araujo and Rui M P Silva-Junior and Tong Zhang and Cara R Schiavon and Qian Chu and Melissa Wu and Carmen L S Pontes and Anderson O Souza and Luciane C Alberici and Aline M Santos and Sadie Bartholomew Ingle and Alan Saghatelian and James Spudich and Uri Manor and Enilza M Espreafico},\r\n\ttitle = {A novel role for Myosin-Va in mitochondrial fission},\r\n\telocation-id = {655803},\r\n\tyear = {2019},\r\n\tdoi = {10.1101/655803},\r\n\tpublisher = {Cold Spring Harbor Laboratory},\r\n\tabstract = {In cancer cells metabolic changes and mitochondrial morphology are coupled. It is known that the cytoskeleton and molecular motors are directly involved in regulating mitochondrial morphology. Here we show that myosin-Va, an actin-based molecular motor, is required for the malignant properties of melanoma cells and localizes to mitochondria in these cells. Knockdown of myosin-Va increases cellular respiration rates and ROS production and decreases glucose uptake and lactate secretion. In addition, knockdown of myosin-Va results in reduced mitochondrial fission and correspondingly elongated mitochondria. We show that myosin-Va interacts with the mitochondrial outer membrane protein Spire1C, an actin-regulatory protein implicated in mitochondrial fission, and that Spire1C recruits myosin-Va to mitochondria. Finally, we show that during mitochondrial fission myosin-Va localization to mitochondria increases, and that myosin-Va localizes to mitochondrial fission sites immediately adjacent to Drp1 punctae. We conclude that myosin-Va facilitates mitochondrial fission. These data implicate myosin-Va as a target for the Warburg effect in melanoma cells.},\r\n\tURL = {https://www.biorxiv.org/content/early/2019/05/31/655803},\r\n\teprint = {https://www.biorxiv.org/content/early/2019/05/31/655803.full.pdf},\r\n\tjournal = {bioRxiv}\r\n}\r\n\r\n","author_short":["Araujo, J. S","Silva-Junior, R. M P","Zhang, T.","Schiavon, C. R","Chu, Q.","Wu, M.","Pontes, C. L S","Souza, A. O","Alberici, L. C","Santos, A. M","Ingle, S. B.","Saghatelian, A.","Spudich, J.","Manor, U.","Espreafico, E. 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