@article{RN188,
   author = {Baah-Dwomoh, A. and Rolong, A. and Gatenholm, P. and Davalos, R. V.},
   title = {The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering},
   journal = {Appl Microbiol Biotechnol},
   volume = {99},
   number = {11},
   pages = {4785-94},
   note = {1432-0614
Baah-Dwomoh, Adwoa
Rolong, Andrea
Gatenholm, Paul
Davalos, Rafael V
R43 AG044153/AG/NIA NIH HHS/United States
R43 AG044153-01A1/AG/NIA NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Germany
2015/02/19
Appl Microbiol Biotechnol. 2015 Jun;99(11):4785-94. doi: 10.1007/s00253-015-6445-0. Epub 2015 Feb 18.},
   abstract = {This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5 kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most of the bacteria and create a localized pore. However, an applied electric field of 17.5 kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects.},
   keywords = {Cellulose/*chemistry/*metabolism
*Electroporation
Gluconacetobacter/*metabolism
Microbial Viability
Nanofibers/*chemistry
Tissue Engineering/*methods
*Tissue Scaffolds},
   ISSN = {0175-7598 (Print)
0175-7598},
   DOI = {10.1007/s00253-015-6445-0},
   year = {2015},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Baah-Dwomoh"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Rolong"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Gatenholm"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering","journal":"Appl Microbiol Biotechnol","volume":"99","number":"11","pages":"4785-94","note":"1432-0614 Baah-Dwomoh, Adwoa Rolong, Andrea Gatenholm, Paul Davalos, Rafael V R43 AG044153/AG/NIA NIH HHS/United States R43 AG044153-01A1/AG/NIA NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Germany 2015/02/19 Appl Microbiol Biotechnol. 2015 Jun;99(11):4785-94. doi: 10.1007/s00253-015-6445-0. Epub 2015 Feb 18.","abstract":"This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5 kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most of the bacteria and create a localized pore. However, an applied electric field of 17.5 kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects.","keywords":"Cellulose/*chemistry/*metabolism *Electroporation Gluconacetobacter/*metabolism Microbial Viability Nanofibers/*chemistry Tissue Engineering/*methods *Tissue Scaffolds","issn":"0175-7598 (Print) 0175-7598","doi":"10.1007/s00253-015-6445-0","year":"2015","bibtex":"@article{RN188,\n author = {Baah-Dwomoh, A. and Rolong, A. and Gatenholm, P. and Davalos, R. V.},\n title = {The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering},\n journal = {Appl Microbiol Biotechnol},\n volume = {99},\n number = {11},\n pages = {4785-94},\n note = {1432-0614\nBaah-Dwomoh, Adwoa\nRolong, Andrea\nGatenholm, Paul\nDavalos, Rafael V\nR43 AG044153/AG/NIA NIH HHS/United States\nR43 AG044153-01A1/AG/NIA NIH HHS/United States\nJournal Article\nResearch Support, N.I.H., Extramural\nResearch Support, U.S. Gov't, Non-P.H.S.\nGermany\n2015/02/19\nAppl Microbiol Biotechnol. 2015 Jun;99(11):4785-94. doi: 10.1007/s00253-015-6445-0. Epub 2015 Feb 18.},\n abstract = {This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5 kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most of the bacteria and create a localized pore. However, an applied electric field of 17.5 kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects.},\n keywords = {Cellulose/*chemistry/*metabolism\n*Electroporation\nGluconacetobacter/*metabolism\nMicrobial Viability\nNanofibers/*chemistry\nTissue Engineering/*methods\n*Tissue Scaffolds},\n ISSN = {0175-7598 (Print)\n0175-7598},\n DOI = {10.1007/s00253-015-6445-0},\n year = {2015},\n type = {Journal Article}\n}\n\n","author_short":["Baah-Dwomoh, A.","Rolong, A.","Gatenholm, P.","Davalos, R. 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