Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses. Bacete, L., Mélida, H., Pattathil, S., Hahn, M. G., Molina, A., & Miedes, E. In Shan, L. & He, P., editors, Plant Pattern Recognition Receptors: Methods and Protocols, of Methods in Molecular Biology, pages 13–23. Springer, New York, NY, 2017. ![Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of Damage-Associated Molecular Patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, such as oligogalacturonic acids (OGs), have been characterized so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterize the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs.
@incollection{bacete_characterization_2017,
address = {New York, NY},
series = {Methods in {Molecular} {Biology}},
title = {Characterization of {Plant} {Cell} {Wall} {Damage}-{Associated} {Molecular} {Patterns} {Regulating} {Immune} {Responses}},
isbn = {978-1-4939-6859-6},
url = {https://doi.org/10.1007/978-1-4939-6859-6_2},
abstract = {The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of Damage-Associated Molecular Patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, such as oligogalacturonic acids (OGs), have been characterized so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterize the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs.},
language = {en},
urldate = {2023-03-10},
booktitle = {Plant {Pattern} {Recognition} {Receptors}: {Methods} and {Protocols}},
publisher = {Springer},
author = {Bacete, Laura and Mélida, Hugo and Pattathil, Sivakumar and Hahn, Michael G. and Molina, Antonio and Miedes, Eva},
editor = {Shan, Libo and He, Ping},
year = {2017},
doi = {10.1007/978-1-4939-6859-6_2},
keywords = {Arabidopsis, Cell wall, Hemicellulose, Immunity, Pectin},
pages = {13--23},
}
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