@article{
 title = {A "novel" protocol for the analysis of hydroxycinnamic acids in leaf tissue of chicory (Cichorium intybus L., Asteraceae).},
 type = {article},
 year = {2012},
 keywords = {Asteraceae,Chemistry, Pharmaceutical,Chemistry, Pharmaceutical: methods,Chemistry, Pharmaceutical: trends,Chicory,Chromatography, High Pressure Liquid,Chromatography, High Pressure Liquid: methods,Chromatography, Liquid,Chromatography, Liquid: methods,Coumaric Acids,Coumaric Acids: analysis,Coumaric Acids: chemistry,Mass Spectrometry,Mass Spectrometry: methods,Plant Leaves},
 pages = {142983},
 volume = {2012},
 websites = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3523586&tool=pmcentrez&rendertype=abstract},
 id = {fddd8775-ace5-3d8b-ad68-84cb46c7a899},
 created = {2016-06-13T20:02:11.000Z},
 file_attached = {false},
 profile_id = {bae88dda-ac8b-323a-8293-ff3f78f068fe},
 last_modified = {2016-06-13T20:03:22.000Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
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 abstract = {A "novel" protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates-caftaric, chlorogenic, and chicoric acids-was observed, during maceration at ambient temperatures, or after storage for 1 year.},
 bibtype = {article},
 author = {Bahri, Meriem and Hance, Philippe and Grec, Sébastien and Quillet, Marie-Christine and Trotin, Francis and Hilbert, Jean-Louis and Hendriks, Theo},
 journal = {TheScientificWorldJournal}
}

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Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates-caftaric, chlorogenic, and chicoric acids-was observed, during maceration at ambient temperatures, or after storage for 1 year.","bibtype":"article","author":"Bahri, Meriem and Hance, Philippe and Grec, Sébastien and Quillet, Marie-Christine and Trotin, Francis and Hilbert, Jean-Louis and Hendriks, Theo","journal":"TheScientificWorldJournal","bibtex":"@article{\n title = {A \"novel\" protocol for the analysis of hydroxycinnamic acids in leaf tissue of chicory (Cichorium intybus L., Asteraceae).},\n type = {article},\n year = {2012},\n keywords = {Asteraceae,Chemistry, Pharmaceutical,Chemistry, Pharmaceutical: methods,Chemistry, Pharmaceutical: trends,Chicory,Chromatography, High Pressure Liquid,Chromatography, High Pressure Liquid: methods,Chromatography, Liquid,Chromatography, Liquid: methods,Coumaric Acids,Coumaric Acids: analysis,Coumaric Acids: chemistry,Mass Spectrometry,Mass Spectrometry: methods,Plant Leaves},\n pages = {142983},\n volume = {2012},\n websites = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3523586&tool=pmcentrez&rendertype=abstract},\n id = {fddd8775-ace5-3d8b-ad68-84cb46c7a899},\n created = {2016-06-13T20:02:11.000Z},\n file_attached = {false},\n profile_id = {bae88dda-ac8b-323a-8293-ff3f78f068fe},\n last_modified = {2016-06-13T20:03:22.000Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n abstract = {A \"novel\" protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. 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