@article{
 title = {A "novel" protocol for the analysis of hydroxycinnamic acids in leaf tissue of chicory (<i>Cichorium intybus</i> L., Asteraceae).},
 type = {article},
 year = {2012},
 keywords = {Asteraceae,Chemistry,Chicory,Chromatography,Coumaric Acids,Coumaric Acids: analysis,Coumaric Acids: chemistry,High Pressure Liquid,High Pressure Liquid: methods,Liquid,Liquid: methods,Mass Spectrometry,Mass Spectrometry: methods,Pharmaceutical,Pharmaceutical: methods,Pharmaceutical: trends,Plant Leaves},
 pages = {142983},
 volume = {2012},
 websites = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3523586&tool=pmcentrez&rendertype=abstract},
 id = {fddd8775-ace5-3d8b-ad68-84cb46c7a899},
 created = {2016-06-13T20:02:11.000Z},
 file_attached = {false},
 profile_id = {bae88dda-ac8b-323a-8293-ff3f78f068fe},
 last_modified = {2021-05-17T16:22:19.566Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 citation_key = {Bahri2012},
 private_publication = {false},
 abstract = {A "novel" protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates-caftaric, chlorogenic, and chicoric acids-was observed, during maceration at ambient temperatures, or after storage for 1 year.},
 bibtype = {article},
 author = {Bahri, Meriem and Hance, Philippe and Grec, Sébastien and Quillet, Marie-Christine and Trotin, Francis and Hilbert, Jean-Louis and Hendriks, Theo},
 doi = {10.1100/2012/142983},
 journal = {The Scientific World Journal}
}

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