@InProceedings{8081245,
  author = {L. Baiazitova and V. Čmiel and J. Skopalík and O. Svoboda and I. Provazník},
  booktitle = {2017 25th European Signal Processing Conference (EUSIPCO)},
  title = {Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells},
  year = {2017},
  pages = {439-443},
  abstract = {Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested. The method was tested on isolated cardiomyocyte cells (CMs) and rat bone marrow stromal cells (MSCs) labelled with SPIO-rhodamine nanoparticles and stained with standard fluorescent dyes. We proved it is possible to use an exponential decrease of fluorescence in time and lifetime parameters for pseudo-colour 3D image mapping of living cells and their compartments that is not a standard function of confocal microscopes.},
  keywords = {biomedical optical imaging;bone;cellular biophysics;dyes;fluorescence;iron compounds;medical image processing;nanomagnetics;nanomedicine;nanoparticles;optical microscopy;superparamagnetism;fluorescent dyes;optical method;three-dimensional fluorescence lifetime imaging;cardiomyocyte cells;SPIO-rhodamine nanoparticles;confocal microscopy;pseudocolour 3D image mapping;lifetime parameters;rat bone marrow stromal cells;image processing steps;quasiFLIM image calculation;living cells;Fe2O3;Fluorescence;Microscopy;Bars;Image segmentation;Bones;Nanoparticles;fluorescence lifetime;FLIM;cardiomyocyte;stromal cell;image segmentation},
  doi = {10.23919/EUSIPCO.2017.8081245},
  issn = {2076-1465},
  month = {Aug},
  url = {https://www.eurasip.org/proceedings/eusipco/eusipco2017/papers/1570347399.pdf},
}

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This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested. The method was tested on isolated cardiomyocyte cells (CMs) and rat bone marrow stromal cells (MSCs) labelled with SPIO-rhodamine nanoparticles and stained with standard fluorescent dyes. 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Baiazitova and V. Čmiel and J. Skopalík and O. Svoboda and I. Provazník},\n booktitle = {2017 25th European Signal Processing Conference (EUSIPCO)},\n title = {Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells},\n year = {2017},\n pages = {439-443},\n abstract = {Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested. The method was tested on isolated cardiomyocyte cells (CMs) and rat bone marrow stromal cells (MSCs) labelled with SPIO-rhodamine nanoparticles and stained with standard fluorescent dyes. 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