Fluorescent Heterotelechelic Single-Chain Polymer Nanoparticles: Synthesis, Spectroscopy, and Cellular Imaging. Bajj, D. N. F., Tran, M. V., Tsai, H., Kim, H., Paisley, N. R., Algar, W. R., & Hudson, Z. M. ACS Applied Nano Materials, 2(2):898–909, American Chemical Society, February, 2019. ![Fluorescent Heterotelechelic Single-Chain Polymer Nanoparticles: Synthesis, Spectroscopy, and Cellular Imaging [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The folding and collapse of individual polymer chains into single-chain polymer nanoparticles (SCPNs) is a versatile and emerging platform for biological applications such as diagnostics, imaging, and therapy, where components for two or more of these functions can be combined onto a single polymer carrier. Here, we prepare heterotelechelic polymers with three sets of separately addressable chemical handles at their a-terminus and ?-terminus, and along their backbone. As a model system, the a- and ?-termini are conjugated with a targeting ligand (folic acid or biotin) and therapeutic drug cargo (camptothecin), respectively, and the backbone is grafted with pendant fluorescent dye molecules, poly(ethylene glycol) oligomers, and benzene-1,3,5-tricarboxamide. These polymers fold in water to give fluorescent SCPNs, which are characterized with respect to their physical and photophysical properties. The latter reveals a relationship between polymer folding, quantum yield, and resistance to photobleaching. The SCPNs are then shown to be useful for immunolabeling of SK-BR-3 breast cancer cells and exhibit little or no acute cytotoxicity. This work demonstrates that SCPNs can be used as a viable platform for bioconjugation and cell labeling, helps establish a set of design criteria for optimizing future biological applications, and opens the door to the development of SCPNs for a broader range of theranostic applications.
@Article{Bajj2019,
author = {Bajj, Daniel N. F. and Tran, Michael V. and Tsai, Hsin-Yun and Kim, Hyungki and Paisley, Nathan R. and Algar, W. Russ and Hudson, Zachary M.},
journal = {ACS Applied Nano Materials},
title = {Fluorescent {Heterotelechelic} {Single}-{Chain} {Polymer} {Nanoparticles}: {Synthesis}, {Spectroscopy}, and {Cellular} {Imaging}},
year = {2019},
month = feb,
number = {2},
pages = {898--909},
volume = {2},
abstract = {The folding and collapse of individual polymer chains into single-chain polymer nanoparticles (SCPNs) is a versatile and emerging platform for biological applications such as diagnostics, imaging, and therapy, where components for two or more of these functions can be combined onto a single polymer carrier. Here, we prepare heterotelechelic polymers with three sets of separately addressable chemical handles at their a-terminus and ?-terminus, and along their backbone. As a model system, the a- and ?-termini are conjugated with a targeting ligand (folic acid or biotin) and therapeutic drug cargo (camptothecin), respectively, and the backbone is grafted with pendant fluorescent dye molecules, poly(ethylene glycol) oligomers, and benzene-1,3,5-tricarboxamide. These polymers fold in water to give fluorescent SCPNs, which are characterized with respect to their physical and photophysical properties. The latter reveals a relationship between polymer folding, quantum yield, and resistance to photobleaching. The SCPNs are then shown to be useful for immunolabeling of SK-BR-3 breast cancer cells and exhibit little or no acute cytotoxicity. This work demonstrates that SCPNs can be used as a viable platform for bioconjugation and cell labeling, helps establish a set of design criteria for optimizing future biological applications, and opens the door to the development of SCPNs for a broader range of theranostic applications.},
doi = {10.1021/acsanm.8b02149},
file = {Full Text PDF:https\://pubs.acs.org/doi/pdf/10.1021/acsanm.8b02149:application/pdf;ACS Full Text Snapshot:https\://pubs.acs.org/doi/full/10.1021/acsanm.8b02149:text/html},
publisher = {American Chemical Society},
shorttitle = {Fluorescent {Heterotelechelic} {Single}-{Chain} {Polymer} {Nanoparticles}},
url = {https://doi.org/10.1021/acsanm.8b02149},
urldate = {2020-06-22TZ},
}
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