The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device. Baker, A. N., Richards, S., Guy, C. S., Congdon, T. R., Hasan, M., Zwetsloot, A. J., Gallo, A., Lewandowski, J. R., Stansfeld, P. J., Straube, A., Walker, M., Chessa, S., Pergolizzi, G., Dedola, S., Field, R. A., & Gibson, I ACS CENTRAL SCIENCE, 6(11):2046–2052, November, 2020. Iceni Diagnost Ltddoi abstract bibtex There is an urgent need to understand the behavior of of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
@article{baker_sars-cov-2_2020,
title = {The {SARS}-{COV}-2 {Spike} {Protein} {Binds} {Sialic} {Acids} and {Enables} {Rapid} {Detection} in a {Lateral} {Flow} {Point} of {Care} {Diagnostic} {Device}},
volume = {6},
issn = {2374-7943},
doi = {10.1021/acscentsci.0c00855},
abstract = {There is an urgent need to understand the behavior of of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.},
number = {11},
urldate = {2020-11-25},
journal = {ACS CENTRAL SCIENCE},
author = {Baker, Alexander N. and Richards, Sarah-Jane and Guy, Collette S. and Congdon, Thomas R. and Hasan, Muhammad and Zwetsloot, Alexander J. and Gallo, Angelo and Lewandowski, Jozef R. and Stansfeld, Phillip J. and Straube, Anne and Walker, Marc and Chessa, Simona and Pergolizzi, Giulia and Dedola, Simone and Field, Robert A. and Gibson, I, Matthew},
month = nov,
year = {2020},
note = {Iceni Diagnost Ltd},
pages = {2046--2052},
}
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