Higher interleukin-33 levels in aggressive periodontitis cases. Ballambettu, S. P., Pradeep, A. R., Purushottam, M., & Sen, S. Journal of Indian Society of Periodontology, 23(5):424–429, October, 2019. Place: India
doi  abstract   bibtex   
CONTEXT: Interleukin-33 (IL-33) is a novel alarmin that warns immune cells of tissue destruction in injury or infection. AIMS: This study is aimed at analyzing and correlating the concentration of IL-33 in the gingival crevicular fluid (GCF) and plasma of healthy subjects and chronic and aggressive periodontitis patients with intronic variant single nucleotide polymorphisms (SNPs), rs1157505 (C/G) and rs7044343 (C/T) in the IL-33 gene. SETTINGS AND DESIGN: This is a cross-sectional, biochemical, genetic study. MATERIALS AND METHODS: Ninety subjects were divided into Group H: subjects with healthy periodontium, Group CP: chronic periodontitis patients, and Group GAgP: generalized aggressive periodontitis patients, based on clinical parameters of periodontal inflammation. IL-33 concentration in GCF, as well as plasma, was quantified using enzyme-linked immunosorbent assay. The SNPs rs1157505 and rs7044343 were analyzed using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: A significant difference was found in IL-33 concentration in GCF and plasma between the three groups. GG genotype of IL-33 SNP rs1157505 was associated with the highest GCF and plasma IL-33 concentration and was significantly more in GAgP than healthy or CP groups. IL-33 SNP rs7044343 did not show any such association. All GAgP patients had the highest GCF and plasma concentration of IL-33. CONCLUSIONS: IL-33 may be a potential inflammatory marker of periodontitis. GG genotype of SNP rs1157505 may be associated with GAgP.
@article{ballambettu_higher_2019,
	title = {Higher interleukin-33 levels in aggressive periodontitis cases.},
	volume = {23},
	issn = {0972-124X},
	doi = {10.4103/jisp.jisp_217_19},
	abstract = {CONTEXT: Interleukin-33 (IL-33) is a novel alarmin that warns immune cells of tissue destruction in injury or infection. AIMS: This study is aimed at analyzing and correlating the concentration of IL-33 in the gingival crevicular fluid (GCF) and plasma of healthy subjects and chronic and aggressive periodontitis patients with intronic variant single nucleotide polymorphisms (SNPs), rs1157505 (C/G) and rs7044343 (C/T) in the IL-33 gene. SETTINGS AND DESIGN: This is a cross-sectional, biochemical, genetic study. MATERIALS AND METHODS: Ninety subjects were divided into Group H: subjects with healthy periodontium, Group CP: chronic periodontitis patients, and Group GAgP: generalized aggressive periodontitis patients, based on clinical parameters of periodontal inflammation. IL-33 concentration in GCF, as well as plasma, was quantified using enzyme-linked immunosorbent assay. The SNPs rs1157505 and rs7044343 were analyzed using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: A significant difference was found in IL-33 concentration in GCF and plasma between the three groups. GG genotype of IL-33 SNP rs1157505 was associated with the highest GCF and plasma IL-33 concentration and was significantly more in GAgP than healthy or CP groups. IL-33 SNP rs7044343 did not show any such association. All GAgP patients had the highest GCF and plasma concentration of IL-33. CONCLUSIONS: IL-33 may be a potential inflammatory marker of periodontitis. GG genotype of SNP rs1157505 may be associated with GAgP.},
	language = {eng},
	number = {5},
	journal = {Journal of Indian Society of Periodontology},
	author = {Ballambettu, Sujatha Pai and Pradeep, A. R. and Purushottam, Meera and Sen, Somdatta},
	month = oct,
	year = {2019},
	pmid = {31543615},
	pmcid = {PMC6737856},
	note = {Place: India},
	keywords = {Aggressive periodontitis, chronic periodontitis, genetic polymorphism, gingival crevicular fluid, interleukin},
	pages = {424--429},
}

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