FGF9 and FGF20 maintain the stemness of nephron progenitors in mice and man. Barak, H., Huh, S., Chen, S., Jeanpierre, C., Martinovic, J., Parisot, M., Bole-Feysot, C., Nitschké, P., Salomon, R., Antignac, C., Ornitz, D. M., & Kopan, R. Developmental Cell, 22(6):1191–1207, June, 2012. doi abstract bibtex The identity of niche signals necessary to maintain embryonic nephron progenitors is unclear. Here we provide evidence that Fgf20 and Fgf9, expressed in the niche, and Fgf9, secreted from the adjacent ureteric bud, are necessary and sufficient to maintain progenitor stemness. Reduction in the level of these redundant ligands in the mouse led to premature progenitor differentiation within the niche. Loss of FGF20 in humans, or of both ligands in mice, resulted in kidney agenesis. Sufficiency was shown in vitro where Fgf20 or Fgf9 (alone or together with Bmp7) maintained isolated metanephric mesenchyme or sorted nephron progenitors that remained competent to differentiate in response to Wnt signals after 5 or 2 days in culture, respectively. These findings identify a long-sought-after critical component of the nephron stem cell niche and hold promise for long-term culture and utilization of these progenitors in vitro.
@article{barak_fgf9_2012,
title = {{FGF9} and {FGF20} maintain the stemness of nephron progenitors in mice and man},
volume = {22},
issn = {1878-1551},
doi = {10.1016/j.devcel.2012.04.018},
abstract = {The identity of niche signals necessary to maintain embryonic nephron progenitors is unclear. Here we provide evidence that Fgf20 and Fgf9, expressed in the niche, and Fgf9, secreted from the adjacent ureteric bud, are necessary and sufficient to maintain progenitor stemness. Reduction in the level of these redundant ligands in the mouse led to premature progenitor differentiation within the niche. Loss of FGF20 in humans, or of both ligands in mice, resulted in kidney agenesis. Sufficiency was shown in vitro where Fgf20 or Fgf9 (alone or together with Bmp7) maintained isolated metanephric mesenchyme or sorted nephron progenitors that remained competent to differentiate in response to Wnt signals after 5 or 2 days in culture, respectively. These findings identify a long-sought-after critical component of the nephron stem cell niche and hold promise for long-term culture and utilization of these progenitors in vitro.},
language = {eng},
number = {6},
journal = {Developmental Cell},
author = {Barak, Hila and Huh, Sung-Ho and Chen, Shuang and Jeanpierre, Cécile and Martinovic, Jelena and Parisot, Mélanie and Bole-Feysot, Christine and Nitschké, Patrick and Salomon, Rémi and Antignac, Corinne and Ornitz, David M. and Kopan, Raphael},
month = jun,
year = {2012},
pmid = {22698282},
pmcid = {PMC3376351},
keywords = {Nephrons, Animals, Mice, Kidney, Mesenchymal Stem Cells, Female, Humans, Male, Mutation, Cell Differentiation, Organ Culture Techniques, Kidney Diseases, Wnt Signaling Pathway, Bone Morphogenetic Protein 7, Congenital Abnormalities, Fibroblast Growth Factor 9, Fibroblast Growth Factors, Stem Cell Niche},
pages = {1191--1207},
file = {Full Text:/Users/cristina/Zotero/storage/5PB6F8VX/Barak et al. - 2012 - FGF9 and FGF20 maintain the stemness of nephron pr.pdf:application/pdf},
}
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Loss of FGF20 in humans, or of both ligands in mice, resulted in kidney agenesis. Sufficiency was shown in vitro where Fgf20 or Fgf9 (alone or together with Bmp7) maintained isolated metanephric mesenchyme or sorted nephron progenitors that remained competent to differentiate in response to Wnt signals after 5 or 2 days in culture, respectively. 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