Integrity of xylan backbone affects plant responses to drought. Barbut, F. R., Cavel, E., Donev, E. N., Gaboreanu, I., Urbancsok, J., Pandey, G., Demailly, H., Jiao, D., Yassin, Z., Derba-Maceluch, M., Master, E. R., Scheepers, G., Gutierrez, L., & Mellerowicz, E. J. Frontiers in Plant Science, June, 2024. Publisher: Frontiers
Integrity of xylan backbone affects plant responses to drought [link]Paper  doi  abstract   bibtex   
\textlessp\textgreaterDrought is a major factor affecting crops, thus efforts are needed to increase plant resilience to this abiotic stress. The overlapping signaling pathways between drought and cell wall integrity maintenance responses create a possibility of increasing drought resistance by modifying cell walls. Here, using herbaceous and woody plant model species, \textlessitalic\textgreaterArabidopsis\textless/italic\textgreater and hybrid aspen, respectively, we investigated how the integrity of xylan in secondary walls affects the responses of plants to drought stress. Plants, in which secondary wall xylan integrity was reduced by expressing fungal GH10 and GH11 xylanases or by affecting genes involved in xylan backbone biosynthesis, were subjected to controlled drought while their physiological responses were continuously monitored by RGB, fluorescence, and/or hyperspectral cameras. For \textlessitalic\textgreaterArabidopsis\textless/italic\textgreater, this was supplemented with survival test after complete water withdrawal and analyses of stomatal function and stem conductivity. All \textlessitalic\textgreaterArabidopsis\textless/italic\textgreater xylan-impaired lines showed better survival upon complete watering withdrawal, increased stomatal density and delayed growth inhibition by moderate drought, indicating increased resilience to moderate drought associated with modified xylan integrity. Subtle differences were recorded between xylan biosynthesis mutants (\textlessitalic\textgreaterirx9\textless/italic\textgreater, \textlessitalic\textgreaterirx10\textless/italic\textgreater and \textlessitalic\textgreaterirx14\textless/italic\textgreater) and xylanase-expressing lines. \textlessitalic\textgreaterirx14\textless/italic\textgreater was the most drought resistant genotype, and the only genotype with increased lignin content and unaltered xylem conductivity despite its \textlessitalic\textgreaterirx\textless/italic\textgreater phenotype. Rosette growth was more affected by drought in GH11- than in GH10-expressing plants. In aspen, mild downregulation of \textlessitalic\textgreaterGT43B\textless/italic\textgreater and \textlessitalic\textgreaterC\textless/italic\textgreater genes did not affect drought responses and the transgenic plants grew better than the wild-type in drought and well-watered conditions. Both GH10 and GH11 xylanases strongly inhibited stem elongation and root growth in well-watered conditions but growth was less inhibited by drought in GH11-expressing plants than in wild-type. Overall, plants with xylan integrity impairment in secondary walls were less affected than wild-type by moderately reduced water availability but their responses also varied among genotypes and species. Thus, modifying the secondary cell wall integrity can be considered as a potential strategy for developing crops better suited to withstand water scarcity, but more research is needed to address the underlying molecular causes of this variability.\textless/p\textgreater
@article{barbut_integrity_2024,
	title = {Integrity of xylan backbone affects plant responses to drought},
	volume = {15},
	issn = {1664-462X},
	url = {https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1422701/full},
	doi = {10.3389/fpls.2024.1422701},
	abstract = {{\textless}p{\textgreater}Drought is a major factor affecting crops, thus efforts are needed to increase plant resilience to this abiotic stress. The overlapping signaling pathways between drought and cell wall integrity maintenance responses create a possibility of increasing drought resistance by modifying cell walls. Here, using herbaceous and woody plant model species, {\textless}italic{\textgreater}Arabidopsis{\textless}/italic{\textgreater} and hybrid aspen, respectively, we investigated how the integrity of xylan in secondary walls affects the responses of plants to drought stress. Plants, in which secondary wall xylan integrity was reduced by expressing fungal GH10 and GH11 xylanases or by affecting genes involved in xylan backbone biosynthesis, were subjected to controlled drought while their physiological responses were continuously monitored by RGB, fluorescence, and/or hyperspectral cameras. For {\textless}italic{\textgreater}Arabidopsis{\textless}/italic{\textgreater}, this was supplemented with survival test after complete water withdrawal and analyses of stomatal function and stem conductivity. All {\textless}italic{\textgreater}Arabidopsis{\textless}/italic{\textgreater} xylan-impaired lines showed better survival upon complete watering withdrawal, increased stomatal density and delayed growth inhibition by moderate drought, indicating increased resilience to moderate drought associated with modified xylan integrity. Subtle differences were recorded between xylan biosynthesis mutants ({\textless}italic{\textgreater}irx9{\textless}/italic{\textgreater}, {\textless}italic{\textgreater}irx10{\textless}/italic{\textgreater} and {\textless}italic{\textgreater}irx14{\textless}/italic{\textgreater}) and xylanase-expressing lines. {\textless}italic{\textgreater}irx14{\textless}/italic{\textgreater} was the most drought resistant genotype, and the only genotype with increased lignin content and unaltered xylem conductivity despite its {\textless}italic{\textgreater}irx{\textless}/italic{\textgreater} phenotype. Rosette growth was more affected by drought in GH11- than in GH10-expressing plants. In aspen, mild downregulation of {\textless}italic{\textgreater}GT43B{\textless}/italic{\textgreater} and {\textless}italic{\textgreater}C{\textless}/italic{\textgreater} genes did not affect drought responses and the transgenic plants grew better than the wild-type in drought and well-watered conditions. Both GH10 and GH11 xylanases strongly inhibited stem elongation and root growth in well-watered conditions but growth was less inhibited by drought in GH11-expressing plants than in wild-type. Overall, plants with xylan integrity impairment in secondary walls were less affected than wild-type by moderately reduced water availability but their responses also varied among genotypes and species. Thus, modifying the secondary cell wall integrity can be considered as a potential strategy for developing crops better suited to withstand water scarcity, but more research is needed to address the underlying molecular causes of this variability.{\textless}/p{\textgreater}},
	language = {English},
	urldate = {2024-07-17},
	journal = {Frontiers in Plant Science},
	author = {Barbut, Félix R. and Cavel, Emilie and Donev, Evgeniy N. and Gaboreanu, Ioana and Urbancsok, János and Pandey, Garima and Demailly, Hervé and Jiao, Dianyi and Yassin, Zakiya and Derba-Maceluch, Marta and Master, Emma R. and Scheepers, Gerhard and Gutierrez, Laurent and Mellerowicz, Ewa J.},
	month = jun,
	year = {2024},
	note = {Publisher: Frontiers},
	keywords = {Arabidopsis, Drought stress, Glucuronoxylan, Populus, cell wall integrity, high-throughput phenotyping, hyperspectral imaging, secondary cell wall},
}
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