Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells.

Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells. Barradas, A. M.<nbsp>C., Lachmann, K., Hlawacek, G., Frielink, C., Truckenmoller, R., Boerman, O. C., van Gastel, R., Garritsen, H., Thomas, M., Moroni, L., van Blitterswijk, C., & de Boer, J. 8, 2012.

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Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells. [link]

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Numerous studies have shown that the physicochemical properties of biomaterials can control cell activity. Cell adhesion, proliferation, differentiation as well as tissue formation in vivo can be tuned by properties such as the porosity, surface micro- and nanoscale topography and chemical composition of biomaterials. This concept is very appealing for tissue engineering since instructive properties in bioactive materials can be more economical and time efficient than traditional strategies of cell pre-differentiation in vitro prior to implantation. The biomaterial surface, which is easy to modify due to its accessibility, may provide the necessary signals to elicit a certain cellular behavior. Here, we used gas plasma technology at atmospheric pressure to modify the physicochemical properties of polylactic acid and analyzed how this influenced pre-osteoblast proliferation and differentiation. Tetramethylsilane and 3-aminopropyl-trimethoxysilane with helium as a carrier gas or a mixture of nitrogen and hydrogen were discharged to polylactic acid discs to create different surface chemical compositions, hydrophobicity and microscale topographies. Such modifications influenced protein adsorption and pre-osteoblast cell adhesion, proliferation and osteogenic differentiation. Furthermore polylactic acid treated with tetramethylsilane enhanced osteogenic differentiation compared to the other surfaces. This promising surface modification could be further explored for potential development of bone graft substitutes.

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  abstract = {Numerous studies have shown that the physicochemical properties of biomaterials can control cell activity. Cell adhesion, proliferation, differentiation as well as tissue formation in vivo can be tuned by properties such as the porosity, surface micro- and nanoscale topography and chemical composition of biomaterials. This concept is very appealing for tissue engineering since instructive properties in bioactive materials can be more economical and time efficient than traditional strategies of cell pre-differentiation in vitro prior to implantation. The biomaterial surface, which is easy to modify due to its accessibility, may provide the necessary signals to elicit a certain cellular behavior. Here, we used gas plasma technology at atmospheric pressure to modify the physicochemical properties of polylactic acid and analyzed how this influenced pre-osteoblast proliferation and differentiation. Tetramethylsilane and 3-aminopropyl-trimethoxysilane with helium as a carrier gas or a mixture of nitrogen and hydrogen were discharged to polylactic acid discs to create different surface chemical compositions, hydrophobicity and microscale topographies. Such modifications influenced protein adsorption and pre-osteoblast cell adhesion, proliferation and osteogenic differentiation. Furthermore polylactic acid treated with tetramethylsilane enhanced osteogenic differentiation compared to the other surfaces. This promising surface modification could be further explored for potential development of bone graft substitutes.},
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  title = {Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells.},
  series = {Acta biomaterialia},
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  tags = {bio, helium, helium ion microscopy, him},
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  author = {Ana M C {Barradas} and Kristina {Lachmann} and Gregor {Hlawacek} and Cathelijne {Frielink} and Roman {Truckenmoller} and Otto C. {Boerman} and Raoul {van Gastel} and Henk {Garritsen} and Michael {Thomas} and Lorenzo {Moroni} and Clemens {van Blitterswijk} and Jan {de Boer}},
  pages = {2969-77},
  volume = {8},
  doi = {10.1016/j.actbio.2012.04.021},
  url = {http://www.ncbi.nlm.nih.gov/pubmed/22522130},
  type = {Journal Article},
  issn = {1878-7568},
  modified = {1376419514},
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  subdiscipline = {Surface and Interface Physics},
  booktitle = {Acta biomaterialia},
  dateaccessed = {30/01/13}
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