Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100. Basavappa, S., Huang, C., Mangel, A., Lebedev, D., Knauf, P., & Ellory, J. Journal of Neurophysiology, 76(2):764-769, American Physiological Society, 1996. cited By 32![Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100 [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter Multisizer and radiolabeled amino acid efflux, respectively. 2. CHP-100 cells swelled by 35 ± 5% (means ± SE) when the osmolarity of the solution was decreased from 290 to 190 mOsm/kg H2O. The rapid swelling was followed by a biphasic regulatory volume decrease (RVD). 3. In cells loaded with 14C-taurine, hypoosmotic stress induced a 300 ± 22% (n = 23. P < 0.05) increase in taurine efflux compared with controls. This efflux was inhibited by the chloride channel blockers 5-nitro-2-(3-phenylpropylamino)- benzoic acid (NPPB). 4,4'-diisothio-cyanostilbene-2,2'-disulfonic acid (DIDS), niflumic acid and by the volume activated anion channel blocker tamoxifen. In addition, the swelling-activated taurine efflux was dependent upon extracellular calcium. 4. Similarly, in cells loaded with 14C-glycine, hypoosmotic stress significantly increased glycine efflux, which was also sensitive to NPPB. In contrast, efflux of 3H-glutamate was not significantly altered after hypoosmotic stress. 5. With the use of patch clamp recording techniques, Cl- channels were activated in cell attached patches after exposure to hypoosmotic solutions. 6. In nystatin perforated patches, permeability of the hypoosmotically activated anion channel was observed to be SCN > I > Br > Cl >> Glutamate. 7. It is concluded that in CHP-100 cells, anion channels are activated during hypoosmotic stress and these channels represent a pathway for efflux of amino acids.
@ARTICLE{Basavappa1996764,
author={Basavappa, S. and Huang, C.-C. and Mangel, A.W. and Lebedev, D.V. and Knauf, P.A. and Ellory, J.C.},
title={Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100},
journal={Journal of Neurophysiology},
year={1996},
volume={76},
number={2},
pages={764-769},
doi={10.1152/jn.1996.76.2.764},
note={cited By 32},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029813762&doi=10.1152%2fjn.1996.76.2.764&partnerID=40&md5=6062bad593f7a224da629e608419d38a},
affiliation={Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom},
abstract={1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter Multisizer and radiolabeled amino acid efflux, respectively. 2. CHP-100 cells swelled by ~35 ± 5% (means ± SE) when the osmolarity of the solution was decreased from 290 to 190 mOsm/kg H2O. The rapid swelling was followed by a biphasic regulatory volume decrease (RVD). 3. In cells loaded with 14C-taurine, hypoosmotic stress induced a 300 ± 22% (n = 23. P < 0.05) increase in taurine efflux compared with controls. This efflux was inhibited by the chloride channel blockers 5-nitro-2-(3-phenylpropylamino)- benzoic acid (NPPB). 4,4'-diisothio-cyanostilbene-2,2'-disulfonic acid (DIDS), niflumic acid and by the volume activated anion channel blocker tamoxifen. In addition, the swelling-activated taurine efflux was dependent upon extracellular calcium. 4. Similarly, in cells loaded with 14C-glycine, hypoosmotic stress significantly increased glycine efflux, which was also sensitive to NPPB. In contrast, efflux of 3H-glutamate was not significantly altered after hypoosmotic stress. 5. With the use of patch clamp recording techniques, Cl- channels were activated in cell attached patches after exposure to hypoosmotic solutions. 6. In nystatin perforated patches, permeability of the hypoosmotically activated anion channel was observed to be SCN > I > Br > Cl >> Glutamate. 7. It is concluded that in CHP-100 cells, anion channels are activated during hypoosmotic stress and these channels represent a pathway for efflux of amino acids.},
correspondence_address1={Basavappa, S.; Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom},
publisher={American Physiological Society},
issn={00223077},
coden={JONEA},
pubmed_id={8871197},
language={English},
abbrev_source_title={J. NEUROPHYSIOL.},
document_type={Article},
source={Scopus},
}
Downloads: 0
{"_id":"R3E5EoQiW6RpwY5eN","bibbaseid":"basavappa-huang-mangel-lebedev-knauf-ellory-swellingactivatedaminoacideffluxinthehumanneuroblastomacelllinechp100-1996","author_short":["Basavappa, S.","Huang, C.","Mangel, A.","Lebedev, D.","Knauf, P.","Ellory, J."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Basavappa"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Huang"],"firstnames":["C.-C."],"suffixes":[]},{"propositions":[],"lastnames":["Mangel"],"firstnames":["A.W."],"suffixes":[]},{"propositions":[],"lastnames":["Lebedev"],"firstnames":["D.V."],"suffixes":[]},{"propositions":[],"lastnames":["Knauf"],"firstnames":["P.A."],"suffixes":[]},{"propositions":[],"lastnames":["Ellory"],"firstnames":["J.C."],"suffixes":[]}],"title":"Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100","journal":"Journal of Neurophysiology","year":"1996","volume":"76","number":"2","pages":"764-769","doi":"10.1152/jn.1996.76.2.764","note":"cited By 32","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029813762&doi=10.1152%2fjn.1996.76.2.764&partnerID=40&md5=6062bad593f7a224da629e608419d38a","affiliation":"Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom","abstract":"1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter Multisizer and radiolabeled amino acid efflux, respectively. 2. CHP-100 cells swelled by 35 ± 5% (means ± SE) when the osmolarity of the solution was decreased from 290 to 190 mOsm/kg H2O. The rapid swelling was followed by a biphasic regulatory volume decrease (RVD). 3. In cells loaded with 14C-taurine, hypoosmotic stress induced a 300 ± 22% (n = 23. P < 0.05) increase in taurine efflux compared with controls. This efflux was inhibited by the chloride channel blockers 5-nitro-2-(3-phenylpropylamino)- benzoic acid (NPPB). 4,4'-diisothio-cyanostilbene-2,2'-disulfonic acid (DIDS), niflumic acid and by the volume activated anion channel blocker tamoxifen. In addition, the swelling-activated taurine efflux was dependent upon extracellular calcium. 4. Similarly, in cells loaded with 14C-glycine, hypoosmotic stress significantly increased glycine efflux, which was also sensitive to NPPB. In contrast, efflux of 3H-glutamate was not significantly altered after hypoosmotic stress. 5. With the use of patch clamp recording techniques, Cl- channels were activated in cell attached patches after exposure to hypoosmotic solutions. 6. In nystatin perforated patches, permeability of the hypoosmotically activated anion channel was observed to be SCN > I > Br > Cl >> Glutamate. 7. It is concluded that in CHP-100 cells, anion channels are activated during hypoosmotic stress and these channels represent a pathway for efflux of amino acids.","correspondence_address1":"Basavappa, S.; Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom","publisher":"American Physiological Society","issn":"00223077","coden":"JONEA","pubmed_id":"8871197","language":"English","abbrev_source_title":"J. NEUROPHYSIOL.","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Basavappa1996764,\r\nauthor={Basavappa, S. and Huang, C.-C. and Mangel, A.W. and Lebedev, D.V. and Knauf, P.A. and Ellory, J.C.},\r\ntitle={Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100},\r\njournal={Journal of Neurophysiology},\r\nyear={1996},\r\nvolume={76},\r\nnumber={2},\r\npages={764-769},\r\ndoi={10.1152/jn.1996.76.2.764},\r\nnote={cited By 32},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029813762&doi=10.1152%2fjn.1996.76.2.764&partnerID=40&md5=6062bad593f7a224da629e608419d38a},\r\naffiliation={Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom},\r\nabstract={1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter Multisizer and radiolabeled amino acid efflux, respectively. 2. CHP-100 cells swelled by ~35 ± 5% (means ± SE) when the osmolarity of the solution was decreased from 290 to 190 mOsm/kg H2O. The rapid swelling was followed by a biphasic regulatory volume decrease (RVD). 3. In cells loaded with 14C-taurine, hypoosmotic stress induced a 300 ± 22% (n = 23. P < 0.05) increase in taurine efflux compared with controls. This efflux was inhibited by the chloride channel blockers 5-nitro-2-(3-phenylpropylamino)- benzoic acid (NPPB). 4,4'-diisothio-cyanostilbene-2,2'-disulfonic acid (DIDS), niflumic acid and by the volume activated anion channel blocker tamoxifen. In addition, the swelling-activated taurine efflux was dependent upon extracellular calcium. 4. Similarly, in cells loaded with 14C-glycine, hypoosmotic stress significantly increased glycine efflux, which was also sensitive to NPPB. In contrast, efflux of 3H-glutamate was not significantly altered after hypoosmotic stress. 5. With the use of patch clamp recording techniques, Cl- channels were activated in cell attached patches after exposure to hypoosmotic solutions. 6. In nystatin perforated patches, permeability of the hypoosmotically activated anion channel was observed to be SCN > I > Br > Cl >> Glutamate. 7. It is concluded that in CHP-100 cells, anion channels are activated during hypoosmotic stress and these channels represent a pathway for efflux of amino acids.},\r\ncorrespondence_address1={Basavappa, S.; Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom},\r\npublisher={American Physiological Society},\r\nissn={00223077},\r\ncoden={JONEA},\r\npubmed_id={8871197},\r\nlanguage={English},\r\nabbrev_source_title={J. NEUROPHYSIOL.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Basavappa, S.","Huang, C.","Mangel, A.","Lebedev, D.","Knauf, P.","Ellory, J."],"key":"Basavappa1996764","id":"Basavappa1996764","bibbaseid":"basavappa-huang-mangel-lebedev-knauf-ellory-swellingactivatedaminoacideffluxinthehumanneuroblastomacelllinechp100-1996","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029813762&doi=10.1152%2fjn.1996.76.2.764&partnerID=40&md5=6062bad593f7a224da629e608419d38a"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lbm_2019_10.txt","dataSources":["YYLjupmCazkNqWNEJ"],"keywords":[],"search_terms":["swelling","activated","amino","acid","efflux","human","neuroblastoma","cell","line","chp","100","basavappa","huang","mangel","lebedev","knauf","ellory"],"title":"Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100","year":1996}