Nanopore sequencing: From imagination to reality. Bayley, H. Clinical Chemistry, 61(1):25-31, 2015.
Nanopore sequencing: From imagination to reality [pdf]Paper  abstract   bibtex   
DNA sequencing and hence genomics have been transformed over the last decade by the commercialization of inexpensive, massively parallel, short-read, sequencing technology. Nonetheless, a new generation of single-molecule DNA sequencers, which uses nanopore technology, is initiating a further upheaval in genomics. These instruments are portable, capable of reads of over 100 kb, cheap and fast. Nanopore sequencing, a huge technical challenge, took over 25 years to develop. Today's availability of a commercial nanopore sequencing device can be traced to the 1990s when nucleic acid translocation through nanopores was first observed, stochastic sensing developed and the high-resolution structure of a protein nanopore solved. The nanopore platform that has been developed is also capable of the single-molecule detection of a wide variety of additional analytes of medical interest, ranging from small molecules to post-translationally modified proteins.

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