The pulmonary microbiome in sarcoidosis is similar to other parenchymal lung diseases

The pulmonary microbiome in sarcoidosis is similar to other parenchymal lung diseases. Becker, A., Vella, G., Herr, C., Keller, A., Laczny, C., Beisswenger, C., & Bals, R. European Respiratory Journal, 52:1399-3003, European Respiratory Society, November, 2018.
doi abstract bibtex

Sarcoidosis is a chronic inflammatory disease that is characterized by noncaseating granuloma formation and that often affects the lung. The aetiology is largely unclear and there are speculations about a microbial component in the initial activation of adaptive immune mechanisms. The aim of the present study was to determine whether there are differences between the microbiota of patients with sarcoidosis and other parenchymal lung diseases. Patients were recruited at the Department of Internal Medicine V at the Saarland University Medical Center, the study was approved by the ethics committee and informed consent was obtained. Data and biosamples were collected from 31 patients with sarcoidosis and 19 patients with other parenchymal lung diseases (idiopathic pulmonary fibrosis, NSIP). Bronchoalveolar lavage (BAL) was used for the isolation of DNA that was subjected to PCR amplification of the 16sRNA V1V2 and V3V4 gene regions with subsequent sequencing and analysis. The overall quantity of bacterial DNA in all samples was low as compared to samples from patients with acute respiratory tract infection or with chronic obstructive lung disease. Alpha-diversity (Shannon) was not different between the two groups. Also multivariate analysis using principal component analysis (PCA), redundancy analysis (RDA), canonical correspondence analysis (CCA) revealed no significant differences. Microbial community comparisons showed no gross changes of the phyla Firmicutes, Bacteroidetes, Actinobacteria, and others. The data show that the abundance of bacterial DNA in lung samples of patients with sarcoidosis is low and that there are no significant differences between the two patient groups.

@Article{Becker2018,
  author       = {Andre Becker and Giovanna Vella and Christian Herr and Andreas Keller and Cedric Laczny and Christoph Beisswenger and Robert Bals},
  title        = {The pulmonary microbiome in sarcoidosis is similar to other parenchymal lung diseases},
  journal      = {European Respiratory Journal},
  year         = {2018},
  volume       = {52},
  pages        = {1399-3003},
  month        = nov,
  issn         = {1399-3003},
  abstract     = {Sarcoidosis is a chronic inflammatory disease that is characterized by noncaseating granuloma formation and that often affects the lung. The aetiology is largely unclear and there are speculations about a microbial component in the initial activation of adaptive immune mechanisms. The aim of the present study was to determine whether there are differences between the microbiota of patients with sarcoidosis and other parenchymal lung diseases. Patients were recruited at the Department of Internal Medicine V at the Saarland University Medical Center, the study was approved by the ethics committee and informed consent was obtained. Data and biosamples were collected from 31 patients with sarcoidosis and 19 patients with other parenchymal lung diseases (idiopathic pulmonary fibrosis, NSIP). Bronchoalveolar lavage (BAL) was used for the isolation of DNA that was subjected to PCR amplification of the 16sRNA V1V2 and V3V4 gene regions with subsequent sequencing and analysis. The overall quantity of bacterial DNA in all samples was low as compared to samples from patients with acute respiratory tract infection or with chronic obstructive lung disease. Alpha-diversity (Shannon) was not different between the two groups. Also multivariate analysis using principal component analysis (PCA), redundancy analysis (RDA), canonical correspondence analysis (CCA) revealed no significant differences. Microbial community comparisons showed no gross changes of the phyla Firmicutes, Bacteroidetes, Actinobacteria, and others. The data show that the abundance of bacterial DNA in lung samples of patients with sarcoidosis is low and that there are no significant differences between the two patient groups.},
  doi          = {10.1183/13993003.congress-2018.PA5208},
  issn-linking = {1399-3003},
  issue        = {suppl 62},
  pii          = {10.1183/13993003.congress-2018.PA5208},
  publisher    = {European Respiratory Society},
}

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