Proof‐reading 3′→5′ exonucleases isolated from rat liver nuclei. BELYAKOVA, N., KLEINER, N., KRAVETSKAYA, T., LEGINA, O., NARYZHNY, S., PERRINO, F., SHEVELEV, I., & KRUTYAKOV, V. European Journal of Biochemistry, 217(2):493-500, 1993. cited By 34![Proof‐reading 3′→5′ exonucleases isolated from rat liver nuclei [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Mammalian nuclear DNA polymerases α and β are known to be devoid of the editing 3′→5′ exonucleolytic activity. Presumably this activity could be effected by the exonucleases non‐associated covalently with DNA polymerases. Two 3′→5′ exonucleases of 40 kDa and 50 kDa (exo‐40 and exo‐50) have been isolated from rat liver nuclei and purified to near homogeneity. They are shown to excise mismatched nucleotides from poly[d(A‐T)] template, respectively, 10‐fold and 2‐fold faster than the matched ones. Upon addition of either of these exonucleases to the DNA polymerase α from rat liver or calf thymus, the fidelity of in‐vitro reproduction of the primed DNA from bacteriophage φX174 amber 3 is increased 5–10‐fold, levels of exonuclease and DNA‐polymerase activities being similar. Extrapolation of in‐vitro DNA‐replication fidelity to the cellular levels of activities of the exonucleases and the α‐polymerase suggests that exonucleolytic proofreading augments the accuracy of DNA synthesis by 2–3 orders of magnitude. Copyright © 1993, Wiley Blackwell. All rights reserved
@ARTICLE{BELYAKOVA1993493,
author={BELYAKOVA, N.V. and KLEINER, N.E. and KRAVETSKAYA, T.P. and LEGINA, O.K. and NARYZHNY, S.N. and PERRINO, F.W. and SHEVELEV, I.V. and KRUTYAKOV, V.M.},
title={Proof‐reading 3′→5′ exonucleases isolated from rat liver nuclei},
journal={European Journal of Biochemistry},
year={1993},
volume={217},
number={2},
pages={493-500},
doi={10.1111/j.1432-1033.1993.tb18269.x},
note={cited By 34},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027445624&doi=10.1111%2fj.1432-1033.1993.tb18269.x&partnerID=40&md5=a80eebe3e584943ab18fbe4c3960052f},
affiliation={Laboratory of DNA Biosynthesis, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute of the Russia Academy of Sciences, Gatchina, Russian Federation; Bowman Gray School of Medicine, Wake Forest University, Department of Biochemistry, 300 South Hawthorne Road, Winston-Salem, United States},
abstract={Mammalian nuclear DNA polymerases α and β are known to be devoid of the editing 3′→5′ exonucleolytic activity. Presumably this activity could be effected by the exonucleases non‐associated covalently with DNA polymerases. Two 3′→5′ exonucleases of 40 kDa and 50 kDa (exo‐40 and exo‐50) have been isolated from rat liver nuclei and purified to near homogeneity. They are shown to excise mismatched nucleotides from poly[d(A‐T)] template, respectively, 10‐fold and 2‐fold faster than the matched ones. Upon addition of either of these exonucleases to the DNA polymerase α from rat liver or calf thymus, the fidelity of in‐vitro reproduction of the primed DNA from bacteriophage φX174 amber 3 is increased 5–10‐fold, levels of exonuclease and DNA‐polymerase activities being similar. Extrapolation of in‐vitro DNA‐replication fidelity to the cellular levels of activities of the exonucleases and the α‐polymerase suggests that exonucleolytic proofreading augments the accuracy of DNA synthesis by 2–3 orders of magnitude. Copyright © 1993, Wiley Blackwell. All rights reserved},
correspondence_address1={KRUTYAKOV, V.M.; Petersburg Nuclear Physics Institute, Russia Academy of Sciences, Leningrad District, Gatchina, 188350, Russian Federation},
issn={00142956},
pubmed_id={8223593},
language={English},
abbrev_source_title={Eur. J. Biochem.},
document_type={Article},
source={Scopus},
}
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