@incollection{riechmann_isolation_2023,
	address = {New York, NY},
	title = {Isolation of {Nuclei} {Tagged} in {Specific} {Cell} {Types} ({INTACT}) in {Arabidopsis}},
	volume = {2686},
	isbn = {978-1-07-163298-7 978-1-07-163299-4},
	url = {https://link.springer.com/10.1007/978-1-0716-3299-4_16},
	abstract = {Many functionally distinct plant tissues have relatively low numbers of cells that are embedded within complex tissues. For example, the shoot apical meristem (SAM) consists of a small population of pluripotent stem cells surrounded by developing leaves and/or flowers at the growing tip of the plant. It is technically challenging to collect enough high-quality SAM samples for molecular analyses. Isolation of Nuclei Tagged in specific Cell Types (INTACT) is an easily reproducible method that allows the enrichment of biotin-tagged cell-type-specific nuclei from the total nuclei pool using biotin-streptavidin affinity purification. Here, we provide a detailed INTACT protocol for isolating nuclei from the Arabidopsis SAM. One can also adapt this protocol to isolate nuclei from other tissues and cell types for investigating tissue/cell-type-specific transcriptome and epigenome and their changes during developmental programs at a high spatiotemporal resolution. Furthermore, due to its low cost and simple procedures, INTACT can be conducted in any standard molecular laboratory.},
	language = {en},
	urldate = {2023-08-14},
	booktitle = {Flower {Development}},
	author = {Benstein, Ruben M. and Schmid, Markus and You, Yuan},
	editor = {Riechmann, José Luis and Ferrándiz, Cristina},
	month = jan,
	year = {2023},
	doi = {10.1007/978-1-0716-3299-4_16},
	note = {Series Title: Methods in Molecular Biology},
	pages = {313--328},
}

{"_id":"ZDCaAnNYLrTGbdiME","bibbaseid":"benstein-schmid-you-isolationofnucleitaggedinspecificcelltypesintactinarabidopsis-2023","author_short":["Benstein, R. M.","Schmid, M.","You, Y."],"bibdata":{"bibtype":"incollection","type":"incollection","address":"New York, NY","title":"Isolation of Nuclei Tagged in Specific Cell Types (INTACT) in Arabidopsis","volume":"2686","isbn":"978-1-07-163298-7 978-1-07-163299-4","url":"https://link.springer.com/10.1007/978-1-0716-3299-4_16","abstract":"Many functionally distinct plant tissues have relatively low numbers of cells that are embedded within complex tissues. For example, the shoot apical meristem (SAM) consists of a small population of pluripotent stem cells surrounded by developing leaves and/or flowers at the growing tip of the plant. It is technically challenging to collect enough high-quality SAM samples for molecular analyses. Isolation of Nuclei Tagged in specific Cell Types (INTACT) is an easily reproducible method that allows the enrichment of biotin-tagged cell-type-specific nuclei from the total nuclei pool using biotin-streptavidin affinity purification. Here, we provide a detailed INTACT protocol for isolating nuclei from the Arabidopsis SAM. One can also adapt this protocol to isolate nuclei from other tissues and cell types for investigating tissue/cell-type-specific transcriptome and epigenome and their changes during developmental programs at a high spatiotemporal resolution. Furthermore, due to its low cost and simple procedures, INTACT can be conducted in any standard molecular laboratory.","language":"en","urldate":"2023-08-14","booktitle":"Flower Development","author":[{"propositions":[],"lastnames":["Benstein"],"firstnames":["Ruben","M."],"suffixes":[]},{"propositions":[],"lastnames":["Schmid"],"firstnames":["Markus"],"suffixes":[]},{"propositions":[],"lastnames":["You"],"firstnames":["Yuan"],"suffixes":[]}],"editor":[{"propositions":[],"lastnames":["Riechmann"],"firstnames":["José","Luis"],"suffixes":[]},{"propositions":[],"lastnames":["Ferrándiz"],"firstnames":["Cristina"],"suffixes":[]}],"month":"January","year":"2023","doi":"10.1007/978-1-0716-3299-4_16","note":"Series Title: Methods in Molecular Biology","pages":"313–328","bibtex":"@incollection{riechmann_isolation_2023,\n\taddress = {New York, NY},\n\ttitle = {Isolation of {Nuclei} {Tagged} in {Specific} {Cell} {Types} ({INTACT}) in {Arabidopsis}},\n\tvolume = {2686},\n\tisbn = {978-1-07-163298-7 978-1-07-163299-4},\n\turl = {https://link.springer.com/10.1007/978-1-0716-3299-4_16},\n\tabstract = {Many functionally distinct plant tissues have relatively low numbers of cells that are embedded within complex tissues. For example, the shoot apical meristem (SAM) consists of a small population of pluripotent stem cells surrounded by developing leaves and/or flowers at the growing tip of the plant. It is technically challenging to collect enough high-quality SAM samples for molecular analyses. Isolation of Nuclei Tagged in specific Cell Types (INTACT) is an easily reproducible method that allows the enrichment of biotin-tagged cell-type-specific nuclei from the total nuclei pool using biotin-streptavidin affinity purification. Here, we provide a detailed INTACT protocol for isolating nuclei from the Arabidopsis SAM. One can also adapt this protocol to isolate nuclei from other tissues and cell types for investigating tissue/cell-type-specific transcriptome and epigenome and their changes during developmental programs at a high spatiotemporal resolution. Furthermore, due to its low cost and simple procedures, INTACT can be conducted in any standard molecular laboratory.},\n\tlanguage = {en},\n\turldate = {2023-08-14},\n\tbooktitle = {Flower {Development}},\n\tauthor = {Benstein, Ruben M. and Schmid, Markus and You, Yuan},\n\teditor = {Riechmann, José Luis and Ferrándiz, Cristina},\n\tmonth = jan,\n\tyear = {2023},\n\tdoi = {10.1007/978-1-0716-3299-4_16},\n\tnote = {Series Title: Methods in Molecular Biology},\n\tpages = {313--328},\n}\n\n","author_short":["Benstein, R. M.","Schmid, M.","You, Y."],"editor_short":["Riechmann, J. L.","Ferrándiz, C."],"key":"riechmann_isolation_2023","id":"riechmann_isolation_2023","bibbaseid":"benstein-schmid-you-isolationofnucleitaggedinspecificcelltypesintactinarabidopsis-2023","role":"author","urls":{"Paper":"https://link.springer.com/10.1007/978-1-0716-3299-4_16"},"metadata":{"authorlinks":{}}},"bibtype":"incollection","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":[],"search_terms":["isolation","nuclei","tagged","specific","cell","types","intact","arabidopsis","benstein","schmid","you"],"title":"Isolation of Nuclei Tagged in Specific Cell Types (INTACT) in Arabidopsis","year":2023}