Molecular Identification of Tuber Magnatum Ectomycorrhizae in the Field. Bertini, L.; Rossi, I.; Zambonelli, A.; Amicucci, A.; Sacchi, A.; Cecchini, M.; Gregori, G.; and Stocchi, V. 161(1):59–64.
Molecular Identification of Tuber Magnatum Ectomycorrhizae in the Field [link]Paper  doi  abstract   bibtex   
uber ectomycorrhizae in a Tuber magnatum ” truffière”, located in Central Italy, were studied using molecular methods. Specifically, RFLP-ITS analyses, ITS sequencing and specific probes hybridization were used to identify 335 Tuber-like ectomycorrhizal morphotypes. Molecular identification was possible even when distinct morphological characteristics were lacking. For the first time, T. magnatum ectomycorrhizae and other coexisting Tuber species collected in the field were analysed using molecular tools for unambiguous identification. Although the ” truffière” under investigation yields good harvests of T. magnatum fruiting bodies, the percentage of T. magnatum ectomycorrhizae found was very low (less than 4.4\,% of the 335 root tips analysed), whereas the percentages of Tuber maculatum and Tuber rufum were considerably higher (48.9\,% and 19.0\,%, respectively).
@article{bertiniMolecularIdentificationTuber2006,
  title = {Molecular Identification of {{Tuber}} Magnatum Ectomycorrhizae in the Field},
  author = {Bertini, Luana and Rossi, Ismaela and Zambonelli, Alessandra and Amicucci, Antonella and Sacchi, Achille and Cecchini, Matteo and Gregori, Gianluigi and Stocchi, Vilberto},
  date = {2006-01},
  journaltitle = {Microbiological Research},
  volume = {161},
  pages = {59--64},
  issn = {0944-5013},
  doi = {10.1016/j.micres.2005.06.003},
  url = {https://doi.org/10.1016/j.micres.2005.06.003},
  abstract = {uber ectomycorrhizae in a Tuber magnatum ” truffière”, located in Central Italy, were studied using molecular methods. Specifically, RFLP-ITS analyses, ITS sequencing and specific probes hybridization were used to identify 335 Tuber-like ectomycorrhizal morphotypes.

Molecular identification was possible even when distinct morphological characteristics were lacking.

For the first time, T. magnatum ectomycorrhizae and other coexisting Tuber species collected in the field were analysed using molecular tools for unambiguous identification. Although the ” truffière” under investigation yields good harvests of T. magnatum fruiting bodies, the percentage of T. magnatum ectomycorrhizae found was very low (less than 4.4\,\% of the 335 root tips analysed), whereas the percentages of Tuber maculatum and Tuber rufum were considerably higher (48.9\,\% and 19.0\,\%, respectively).},
  keywords = {*imported-from-citeulike-INRMM,~INRMM-MiD:c-13802068,forest-resources,mycorrhizal-fungi,secondary-production},
  number = {1}
}
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