@ARTICLE{Bhaduri2021-qf,
  title    = "An atlas of cortical arealization identifies dynamic molecular
              signatures",
  author   = "Bhaduri, Aparna and Sandoval-Espinosa, Carmen and Otero-Garcia,
              Marcos and Oh, Irene and Yin, Raymund and Eze, Ugomma C and
              Nowakowski, Tomasz J and Kriegstein, Arnold R",
  abstract = "The human brain is subdivided into distinct anatomical
              structures, including the neocortex, which in turn encompasses
              dozens of distinct specialized cortical areas. Early
              morphogenetic gradients are known to establish early brain
              regions and cortical areas, but how early patterns result in
              finer and more discrete spatial differences remains poorly
              understood(1). Here we use single-cell RNA sequencing to profile
              ten major brain structures and six neocortical areas during peak
              neurogenesis and early gliogenesis. Within the neocortex, we find
              that early in the second trimester, a large number of genes are
              differentially expressed across distinct cortical areas in all
              cell types, including radial glia, the neural progenitors of the
              cortex. However, the abundance of areal transcriptomic signatures
              increases as radial glia differentiate into intermediate
              progenitor cells and ultimately give rise to excitatory neurons.
              Using an automated, multiplexed single-molecule fluorescent in
              situ hybridization approach, we find that laminar gene-expression
              patterns are highly dynamic across cortical regions. Together,
              our data suggest that early cortical areal patterning is defined
              by strong, mutually exclusive frontal and occipital
              gene-expression signatures, with resulting gradients giving rise
              to the specification of areas between these two poles throughout
              successive developmental timepoints.",
  journal  = "Nature",
  volume   =  598,
  number   =  7879,
  pages    = "200--204",
  month    =  oct,
  year     =  2021,
  language = "en"
}

{"_id":"wBrQunpcWnoXepzyD","bibbaseid":"bhaduri-sandovalespinosa-oterogarcia-oh-yin-eze-nowakowski-kriegstein-anatlasofcorticalarealizationidentifiesdynamicmolecularsignatures-2021","author_short":["Bhaduri, A.","Sandoval-Espinosa, C.","Otero-Garcia, M.","Oh, I.","Yin, R.","Eze, U. C","Nowakowski, T. J","Kriegstein, A. R"],"bibdata":{"bibtype":"article","type":"article","title":"An atlas of cortical arealization identifies dynamic molecular signatures","author":[{"propositions":[],"lastnames":["Bhaduri"],"firstnames":["Aparna"],"suffixes":[]},{"propositions":[],"lastnames":["Sandoval-Espinosa"],"firstnames":["Carmen"],"suffixes":[]},{"propositions":[],"lastnames":["Otero-Garcia"],"firstnames":["Marcos"],"suffixes":[]},{"propositions":[],"lastnames":["Oh"],"firstnames":["Irene"],"suffixes":[]},{"propositions":[],"lastnames":["Yin"],"firstnames":["Raymund"],"suffixes":[]},{"propositions":[],"lastnames":["Eze"],"firstnames":["Ugomma","C"],"suffixes":[]},{"propositions":[],"lastnames":["Nowakowski"],"firstnames":["Tomasz","J"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]}],"abstract":"The human brain is subdivided into distinct anatomical structures, including the neocortex, which in turn encompasses dozens of distinct specialized cortical areas. Early morphogenetic gradients are known to establish early brain regions and cortical areas, but how early patterns result in finer and more discrete spatial differences remains poorly understood(1). Here we use single-cell RNA sequencing to profile ten major brain structures and six neocortical areas during peak neurogenesis and early gliogenesis. Within the neocortex, we find that early in the second trimester, a large number of genes are differentially expressed across distinct cortical areas in all cell types, including radial glia, the neural progenitors of the cortex. However, the abundance of areal transcriptomic signatures increases as radial glia differentiate into intermediate progenitor cells and ultimately give rise to excitatory neurons. Using an automated, multiplexed single-molecule fluorescent in situ hybridization approach, we find that laminar gene-expression patterns are highly dynamic across cortical regions. Together, our data suggest that early cortical areal patterning is defined by strong, mutually exclusive frontal and occipital gene-expression signatures, with resulting gradients giving rise to the specification of areas between these two poles throughout successive developmental timepoints.","journal":"Nature","volume":"598","number":"7879","pages":"200–204","month":"October","year":"2021","language":"en","bibtex":"@ARTICLE{Bhaduri2021-qf,\n title = \"An atlas of cortical arealization identifies dynamic molecular\n signatures\",\n author = \"Bhaduri, Aparna and Sandoval-Espinosa, Carmen and Otero-Garcia,\n Marcos and Oh, Irene and Yin, Raymund and Eze, Ugomma C and\n Nowakowski, Tomasz J and Kriegstein, Arnold R\",\n abstract = \"The human brain is subdivided into distinct anatomical\n structures, including the neocortex, which in turn encompasses\n dozens of distinct specialized cortical areas. Early\n morphogenetic gradients are known to establish early brain\n regions and cortical areas, but how early patterns result in\n finer and more discrete spatial differences remains poorly\n understood(1). Here we use single-cell RNA sequencing to profile\n ten major brain structures and six neocortical areas during peak\n neurogenesis and early gliogenesis. Within the neocortex, we find\n that early in the second trimester, a large number of genes are\n differentially expressed across distinct cortical areas in all\n cell types, including radial glia, the neural progenitors of the\n cortex. However, the abundance of areal transcriptomic signatures\n increases as radial glia differentiate into intermediate\n progenitor cells and ultimately give rise to excitatory neurons.\n Using an automated, multiplexed single-molecule fluorescent in\n situ hybridization approach, we find that laminar gene-expression\n patterns are highly dynamic across cortical regions. Together,\n our data suggest that early cortical areal patterning is defined\n by strong, mutually exclusive frontal and occipital\n gene-expression signatures, with resulting gradients giving rise\n to the specification of areas between these two poles throughout\n successive developmental timepoints.\",\n journal = \"Nature\",\n volume = 598,\n number = 7879,\n pages = \"200--204\",\n month = oct,\n year = 2021,\n language = \"en\"\n}\n\n","author_short":["Bhaduri, A.","Sandoval-Espinosa, C.","Otero-Garcia, M.","Oh, I.","Yin, R.","Eze, U. C","Nowakowski, T. J","Kriegstein, A. R"],"key":"Bhaduri2021-qf","id":"Bhaduri2021-qf","bibbaseid":"bhaduri-sandovalespinosa-oterogarcia-oh-yin-eze-nowakowski-kriegstein-anatlasofcorticalarealizationidentifiesdynamicmolecularsignatures-2021","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["atlas","cortical","arealization","identifies","dynamic","molecular","signatures","bhaduri","sandoval-espinosa","otero-garcia","oh","yin","eze","nowakowski","kriegstein"],"title":"An atlas of cortical arealization identifies dynamic molecular signatures","year":2021}