The nuclear exosome subunit HEN2 acts independently of the core exosome to assist transcription in Arabidopsis. Bhat, S. S., Asgari, M., Mermet, S., Mishra, P., & Kindgren, P. Plant Physiology, September, 2024. ![The nuclear exosome subunit HEN2 acts independently of the core exosome to assist transcription in Arabidopsis [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Regulation of gene expression is at the frontier of plant responses to various external stimuli including stress. RNA polymerase-based transcription and post-transcriptional degradation of RNA play vital roles in this regulation. Here, we show that HUA ENHANCER 2 (HEN2), a co-factor of the nuclear exosome complex, influences RNAPII transcription elongation in Arabidopsis (Arabidopsis thaliana) under cold conditions. Our results demonstrate that a hen2 mutant is cold sensitive and undergoes substantial transcriptional changes compared to wild type when exposed to cold conditions. We found an accumulation of 5’ fragments from a subset of genes (including C-repeat binding factors 1-3 [CBF1-3]) that do not carry over to their 3’ ends. In fact, hen2 mutants have lower levels of full-length mRNA for a subset of genes. This distinct 5’-end accumulation and 3’-end depletion was not observed in other NEXT complex members or core exosome mutants, highlighting HEN2’s distinctive role. We further used RNAPII-associated nascent RNA to confirm the transcriptional phenotype is a result of lower active transcription specifically at the 3’-end of these genes in a hen2 mutant. Taken together, our data point to the unique role of HEN2 in maintaining RNAPII transcription dynamics especially highlighted under cold stress.
@article{bhat_nuclear_2024,
title = {The nuclear exosome subunit {HEN2} acts independently of the core exosome to assist transcription in {Arabidopsis}},
issn = {0032-0889},
url = {https://doi.org/10.1093/plphys/kiae503},
doi = {10.1093/plphys/kiae503},
abstract = {Regulation of gene expression is at the frontier of plant responses to various external stimuli including stress. RNA polymerase-based transcription and post-transcriptional degradation of RNA play vital roles in this regulation. Here, we show that HUA ENHANCER 2 (HEN2), a co-factor of the nuclear exosome complex, influences RNAPII transcription elongation in Arabidopsis (Arabidopsis thaliana) under cold conditions. Our results demonstrate that a hen2 mutant is cold sensitive and undergoes substantial transcriptional changes compared to wild type when exposed to cold conditions. We found an accumulation of 5’ fragments from a subset of genes (including C-repeat binding factors 1-3 [CBF1-3]) that do not carry over to their 3’ ends. In fact, hen2 mutants have lower levels of full-length mRNA for a subset of genes. This distinct 5’-end accumulation and 3’-end depletion was not observed in other NEXT complex members or core exosome mutants, highlighting HEN2’s distinctive role. We further used RNAPII-associated nascent RNA to confirm the transcriptional phenotype is a result of lower active transcription specifically at the 3’-end of these genes in a hen2 mutant. Taken together, our data point to the unique role of HEN2 in maintaining RNAPII transcription dynamics especially highlighted under cold stress.},
urldate = {2024-09-27},
journal = {Plant Physiology},
author = {Bhat, Susheel Sagar and Asgari, Mishaneh and Mermet, Sarah and Mishra, Priyanka and Kindgren, Peter},
month = sep,
year = {2024},
pages = {kiae503},
}
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Our results demonstrate that a hen2 mutant is cold sensitive and undergoes substantial transcriptional changes compared to wild type when exposed to cold conditions. We found an accumulation of 5’ fragments from a subset of genes (including C-repeat binding factors 1-3 [CBF1-3]) that do not carry over to their 3’ ends. In fact, hen2 mutants have lower levels of full-length mRNA for a subset of genes. This distinct 5’-end accumulation and 3’-end depletion was not observed in other NEXT complex members or core exosome mutants, highlighting HEN2’s distinctive role. We further used RNAPII-associated nascent RNA to confirm the transcriptional phenotype is a result of lower active transcription specifically at the 3’-end of these genes in a hen2 mutant. 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