@article{RN174,
   author = {Bhonsle, S. and Bonakdar, M. and Neal, R. E., 2nd and Aardema, C. and Robertson, J. L. and Howarth, J. and Kavnoudias, H. and Thomson, K. R. and Goldberg, S. N. and Davalos, R. V.},
   title = {Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model},
   journal = {J Vasc Interv Radiol},
   volume = {27},
   number = {12},
   pages = {1913-1922.e2},
   note = {1535-7732
Bhonsle, Suyashree
Bonakdar, Mohammad
Neal, Robert E 2nd
Aardema, Charles
Robertson, John L
Howarth, Jonathon
Kavnoudias, Helen
Thomson, Kenneth R
Goldberg, S Nahum
Davalos, Rafael V
Comparative Study
Journal Article
Validation Study
United States
2016/09/25
J Vasc Interv Radiol. 2016 Dec;27(12):1913-1922.e2. doi: 10.1016/j.jvir.2016.07.012. Epub 2016 Sep 21.},
   abstract = {PURPOSE: To develop and validate a perfused organ model for characterizing ablations for irreversible electroporation (IRE)-based therapies. MATERIALS AND METHODS: Eight excised porcine livers were mechanically perfused with a modified phosphate-buffered saline solution to maintain viability during IRE ablation. IRE pulses were delivered using 2 monopolar electrodes over a range of parameters, including voltage (1,875-3,000 V), pulse length (70-100 µsec), number of pulses (50-600), electrode exposure (1.0-2.0 cm), and electrode spacing (1.5-2.0 cm). Organs were dissected, and treatment zones were stained with triphenyl tetrazolium chloride to demonstrate viability and highlight the area of ablation. Results were compared with 17 in vivo ablations performed in canine livers and 35 previously published ablations performed in porcine livers. RESULTS: Ablation dimensions in the perfused model correlated well with corresponding in vivo ablations (R(2) = 0.9098) with a 95% confidence interval of < 2.2 mm. Additionally, the validated perfused model showed that the IRE ablation zone grew logarithmically with increasing pulse numbers, showing small difference in ablation size over 200-600 pulses (3.2 mm ± 3.8 width and 5.2 mm ± 3.9 height). CONCLUSIONS: The perfused organ model provides an alternative to animal trials for investigation of IRE treatments. It may have an important role in the future development of new devices, algorithms, and techniques for this therapy.},
   keywords = {*Ablation Techniques/adverse effects/instrumentation
Animals
Dogs
Electrodes
*Electroporation/instrumentation
Equipment Design
In Vitro Techniques
Linear Models
Liver/pathology/*surgery
Male
*Perfusion
Species Specificity
Swine
Tissue Survival},
   ISSN = {1051-0443},
   DOI = {10.1016/j.jvir.2016.07.012},
   year = {2016},
   type = {Journal Article}
}

{"_id":"sd9B5xX86udrETmoM","bibbaseid":"bhonsle-bonakdar-neal-aardema-robertson-howarth-kavnoudias-thomson-etal-characterizationofirreversibleelectroporationablationwithavalidatedperfusedorganmodel-2016","author_short":["Bhonsle, S.","Bonakdar, M.","Neal, R. E.","Aardema, C.","Robertson, J. L.","Howarth, J.","Kavnoudias, H.","Thomson, K. R.","Goldberg, S. N.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Bhonsle"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Bonakdar"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Neal"],"firstnames":["R.","E."],"suffixes":["2nd"]},{"propositions":[],"lastnames":["Aardema"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Robertson"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Howarth"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Kavnoudias"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Thomson"],"firstnames":["K.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Goldberg"],"firstnames":["S.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model","journal":"J Vasc Interv Radiol","volume":"27","number":"12","pages":"1913-1922.e2","note":"1535-7732 Bhonsle, Suyashree Bonakdar, Mohammad Neal, Robert E 2nd Aardema, Charles Robertson, John L Howarth, Jonathon Kavnoudias, Helen Thomson, Kenneth R Goldberg, S Nahum Davalos, Rafael V Comparative Study Journal Article Validation Study United States 2016/09/25 J Vasc Interv Radiol. 2016 Dec;27(12):1913-1922.e2. doi: 10.1016/j.jvir.2016.07.012. Epub 2016 Sep 21.","abstract":"PURPOSE: To develop and validate a perfused organ model for characterizing ablations for irreversible electroporation (IRE)-based therapies. MATERIALS AND METHODS: Eight excised porcine livers were mechanically perfused with a modified phosphate-buffered saline solution to maintain viability during IRE ablation. IRE pulses were delivered using 2 monopolar electrodes over a range of parameters, including voltage (1,875-3,000 V), pulse length (70-100 µsec), number of pulses (50-600), electrode exposure (1.0-2.0 cm), and electrode spacing (1.5-2.0 cm). Organs were dissected, and treatment zones were stained with triphenyl tetrazolium chloride to demonstrate viability and highlight the area of ablation. Results were compared with 17 in vivo ablations performed in canine livers and 35 previously published ablations performed in porcine livers. RESULTS: Ablation dimensions in the perfused model correlated well with corresponding in vivo ablations (R(2) = 0.9098) with a 95% confidence interval of < 2.2 mm. Additionally, the validated perfused model showed that the IRE ablation zone grew logarithmically with increasing pulse numbers, showing small difference in ablation size over 200-600 pulses (3.2 mm ± 3.8 width and 5.2 mm ± 3.9 height). CONCLUSIONS: The perfused organ model provides an alternative to animal trials for investigation of IRE treatments. It may have an important role in the future development of new devices, algorithms, and techniques for this therapy.","keywords":"*Ablation Techniques/adverse effects/instrumentation Animals Dogs Electrodes *Electroporation/instrumentation Equipment Design In Vitro Techniques Linear Models Liver/pathology/*surgery Male *Perfusion Species Specificity Swine Tissue Survival","issn":"1051-0443","doi":"10.1016/j.jvir.2016.07.012","year":"2016","bibtex":"@article{RN174,\n author = {Bhonsle, S. and Bonakdar, M. and Neal, R. E., 2nd and Aardema, C. and Robertson, J. L. and Howarth, J. and Kavnoudias, H. and Thomson, K. R. and Goldberg, S. N. and Davalos, R. V.},\n title = {Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model},\n journal = {J Vasc Interv Radiol},\n volume = {27},\n number = {12},\n pages = {1913-1922.e2},\n note = {1535-7732\nBhonsle, Suyashree\nBonakdar, Mohammad\nNeal, Robert E 2nd\nAardema, Charles\nRobertson, John L\nHowarth, Jonathon\nKavnoudias, Helen\nThomson, Kenneth R\nGoldberg, S Nahum\nDavalos, Rafael V\nComparative Study\nJournal Article\nValidation Study\nUnited States\n2016/09/25\nJ Vasc Interv Radiol. 2016 Dec;27(12):1913-1922.e2. doi: 10.1016/j.jvir.2016.07.012. Epub 2016 Sep 21.},\n abstract = {PURPOSE: To develop and validate a perfused organ model for characterizing ablations for irreversible electroporation (IRE)-based therapies. MATERIALS AND METHODS: Eight excised porcine livers were mechanically perfused with a modified phosphate-buffered saline solution to maintain viability during IRE ablation. IRE pulses were delivered using 2 monopolar electrodes over a range of parameters, including voltage (1,875-3,000 V), pulse length (70-100 µsec), number of pulses (50-600), electrode exposure (1.0-2.0 cm), and electrode spacing (1.5-2.0 cm). Organs were dissected, and treatment zones were stained with triphenyl tetrazolium chloride to demonstrate viability and highlight the area of ablation. Results were compared with 17 in vivo ablations performed in canine livers and 35 previously published ablations performed in porcine livers. RESULTS: Ablation dimensions in the perfused model correlated well with corresponding in vivo ablations (R(2) = 0.9098) with a 95% confidence interval of < 2.2 mm. Additionally, the validated perfused model showed that the IRE ablation zone grew logarithmically with increasing pulse numbers, showing small difference in ablation size over 200-600 pulses (3.2 mm ± 3.8 width and 5.2 mm ± 3.9 height). CONCLUSIONS: The perfused organ model provides an alternative to animal trials for investigation of IRE treatments. It may have an important role in the future development of new devices, algorithms, and techniques for this therapy.},\n keywords = {*Ablation Techniques/adverse effects/instrumentation\nAnimals\nDogs\nElectrodes\n*Electroporation/instrumentation\nEquipment Design\nIn Vitro Techniques\nLinear Models\nLiver/pathology/*surgery\nMale\n*Perfusion\nSpecies Specificity\nSwine\nTissue Survival},\n ISSN = {1051-0443},\n DOI = {10.1016/j.jvir.2016.07.012},\n year = {2016},\n type = {Journal Article}\n}\n\n","author_short":["Bhonsle, S.","Bonakdar, M.","Neal, R. E.","Aardema, C.","Robertson, J. L.","Howarth, J.","Kavnoudias, H.","Thomson, K. R.","Goldberg, S. N.","Davalos, R. V."],"key":"RN174","id":"RN174","bibbaseid":"bhonsle-bonakdar-neal-aardema-robertson-howarth-kavnoudias-thomson-etal-characterizationofirreversibleelectroporationablationwithavalidatedperfusedorganmodel-2016","role":"author","urls":{},"keyword":["*Ablation Techniques/adverse effects/instrumentation Animals Dogs Electrodes *Electroporation/instrumentation Equipment Design In Vitro Techniques Linear Models Liver/pathology/*surgery Male *Perfusion Species Specificity Swine Tissue Survival"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["*ablation techniques/adverse effects/instrumentation animals dogs electrodes *electroporation/instrumentation equipment design in vitro techniques linear models liver/pathology/*surgery male *perfusion species specificity swine tissue survival"],"search_terms":["characterization","irreversible","electroporation","ablation","validated","perfused","organ","model","bhonsle","bonakdar","neal","aardema","robertson","howarth","kavnoudias","thomson","goldberg","davalos"],"title":"Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model","year":2016}