Identification of Two Distinct Linear B Cell Epitopes of the Matrix Protein of the Newcastle Disease Virus Vaccine Strain LaSota. Bi, Y., Jin, Z., Wang, Y., Mou, S., Wang, W., Wei, Q., Huo, N., Liu, S., Wang, X., Yang, Z., Chen, H., & Xiao, S. Viral Immunology, 32(5):221–229, June, 2019. Number: 5![Identification of Two Distinct Linear B Cell Epitopes of the Matrix Protein of the Newcastle Disease Virus Vaccine Strain LaSota [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 1 download Matrix (M) protein of Newcastle disease virus (NDV) is an abundant protein that can induce a robust humoral immune response. However, its antigenic epitopes remain unknown. In this study, we used a pepscan approach to map linear B cell immunodominant epitopes (IDEs) of M protein with NDV-specific chicken antisera. The six epitopes with the highest reactivity by peptide scanning were obtained as IDE candidates. Among them, aa71–85 and aa349–363 were identified by immunological assays with NDV-specific or IDE-specific antisera. The minimal antigenic epitopes of the two IDEs were further characterized as 77MIDDKP82 and 354HTLAKYNPFK363. Moreover, an amino acid sequence alignment and immunoblot analysis revealed the conservation of the two IDEs in the M protein of strains of different genotypes. These two IDEs of M protein could be genetically eliminated as negative markers in recombinant NDV for serologically differential diagnosis in the development of marker vaccines.
@article{bi_identification_2019,
title = {Identification of {Two} {Distinct} {Linear} {B} {Cell} {Epitopes} of the {Matrix} {Protein} of the {Newcastle} {Disease} {Virus} {Vaccine} {Strain} {LaSota}},
volume = {32},
issn = {0882-8245, 1557-8976},
url = {https://www.liebertpub.com/doi/10.1089/vim.2019.0007},
doi = {10.1089/vim.2019.0007},
abstract = {Matrix (M) protein of Newcastle disease virus (NDV) is an abundant protein that can induce a robust humoral immune response. However, its antigenic epitopes remain unknown. In this study, we used a pepscan approach to map linear B cell immunodominant epitopes (IDEs) of M protein with NDV-specific chicken antisera. The six epitopes with the highest reactivity by peptide scanning were obtained as IDE candidates. Among them, aa71–85 and aa349–363 were identified by immunological assays with NDV-specific or IDE-specific antisera. The minimal antigenic epitopes of the two IDEs were further characterized as 77MIDDKP82 and 354HTLAKYNPFK363. Moreover, an amino acid sequence alignment and immunoblot analysis revealed the conservation of the two IDEs in the M protein of strains of different genotypes. These two IDEs of M protein could be genetically eliminated as negative markers in recombinant NDV for serologically differential diagnosis in the development of marker vaccines.},
language = {en},
number = {5},
urldate = {2019-12-02},
journal = {Viral Immunology},
author = {Bi, Youkun and Jin, Zhongyuan and Wang, Yanhong and Mou, Sujing and Wang, Wenbin and Wei, Qiaolin and Huo, Na and Liu, Siqi and Wang, Xinglong and Yang, Zengqi and Chen, Hongjun and Xiao, Sa},
month = jun,
year = {2019},
note = {Number: 5},
keywords = {Accessories, Application - Antibody Validation / Epitope Mapping, Application - Infectious Diseases, Application - Vaccine Development, Country - China, Other Organisms, PEPperCHIP - Customized - Linear, Sample Type - Serum},
pages = {221--229},
}
Downloads: 1
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The six epitopes with the highest reactivity by peptide scanning were obtained as IDE candidates. Among them, aa71–85 and aa349–363 were identified by immunological assays with NDV-specific or IDE-specific antisera. The minimal antigenic epitopes of the two IDEs were further characterized as 77MIDDKP82 and 354HTLAKYNPFK363. Moreover, an amino acid sequence alignment and immunoblot analysis revealed the conservation of the two IDEs in the M protein of strains of different genotypes. These two IDEs of M protein could be genetically eliminated as negative markers in recombinant NDV for serologically differential diagnosis in the development of marker vaccines.","language":"en","number":"5","urldate":"2019-12-02","journal":"Viral Immunology","author":[{"propositions":[],"lastnames":["Bi"],"firstnames":["Youkun"],"suffixes":[]},{"propositions":[],"lastnames":["Jin"],"firstnames":["Zhongyuan"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Yanhong"],"suffixes":[]},{"propositions":[],"lastnames":["Mou"],"firstnames":["Sujing"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Wenbin"],"suffixes":[]},{"propositions":[],"lastnames":["Wei"],"firstnames":["Qiaolin"],"suffixes":[]},{"propositions":[],"lastnames":["Huo"],"firstnames":["Na"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Siqi"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Xinglong"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Zengqi"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Hongjun"],"suffixes":[]},{"propositions":[],"lastnames":["Xiao"],"firstnames":["Sa"],"suffixes":[]}],"month":"June","year":"2019","note":"Number: 5","keywords":"Accessories, Application - Antibody Validation / Epitope Mapping, Application - Infectious Diseases, Application - Vaccine Development, Country - China, Other Organisms, PEPperCHIP - Customized - Linear, Sample Type - Serum","pages":"221–229","bibtex":"@article{bi_identification_2019,\n\ttitle = {Identification of {Two} {Distinct} {Linear} {B} {Cell} {Epitopes} of the {Matrix} {Protein} of the {Newcastle} {Disease} {Virus} {Vaccine} {Strain} {LaSota}},\n\tvolume = {32},\n\tissn = {0882-8245, 1557-8976},\n\turl = {https://www.liebertpub.com/doi/10.1089/vim.2019.0007},\n\tdoi = {10.1089/vim.2019.0007},\n\tabstract = {Matrix (M) protein of Newcastle disease virus (NDV) is an abundant protein that can induce a robust humoral immune response. However, its antigenic epitopes remain unknown. In this study, we used a pepscan approach to map linear B cell immunodominant epitopes (IDEs) of M protein with NDV-specific chicken antisera. The six epitopes with the highest reactivity by peptide scanning were obtained as IDE candidates. Among them, aa71–85 and aa349–363 were identified by immunological assays with NDV-specific or IDE-specific antisera. The minimal antigenic epitopes of the two IDEs were further characterized as 77MIDDKP82 and 354HTLAKYNPFK363. Moreover, an amino acid sequence alignment and immunoblot analysis revealed the conservation of the two IDEs in the M protein of strains of different genotypes. 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