Ultra-trace quantification method for chlordecone in human fluids and tissues. Bichon, E.; Guiffard, I.; Vénisseau, A.; Marchand, P.; Antignac, J.; and Le Bizec, B. 1408:169–177.
Ultra-trace quantification method for chlordecone in human fluids and tissues [link]Paper  doi  abstract   bibtex   
Chlordecone is an organochlorine pesticide (OCP) considered as a Persistent Organic Pollutant (POP) as it persists in the environment, bio-accumulates through the food web, causes adverse effects to human health and the environment and transports across international boundaries far from its sources. The atypical physico-chemical properties of chlordecone make its inclusion in classical analytical approaches non applicable. The aim of our work was to include chlordecone in a multi organochlorine residue method preventing any degradation during the analytical process and thus allowing quantification at ppt (ng kg−1 or ng L−1) levels for a wide range of OCPs in breast milk, human serum and adipose tissue. After GC–HRMS vs. MS/MS and EI vs. APCI comparisons, the major improvement in terms of sensitivity was found in decreasing the length and film thickness of the gas chromatography column. Thanks to a linear correlation between relative response and quantity of chlordecone injected, LC-(ESI-)-MS/MS was finally preferred. An acetonitrile based gradient optimized on a C30 coreshell HPLC column has led to reaching limits of quantification as low as 8 ng L−1, 25 pg mL−1 and 0.2 ng g−1 fat for breast milk, serum and adipose tissue, respectively, allowing multiresidue OCP quantification at concentration levels compatible with biomonitoring purposes and pre-requisites.
@article{bichon_ultra-trace_2015,
	title = {Ultra-trace quantification method for chlordecone in human fluids and tissues},
	volume = {1408},
	issn = {00219673},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0021967315009644},
	doi = {10.1016/j.chroma.2015.07.013},
	abstract = {Chlordecone is an organochlorine pesticide ({OCP}) considered as a Persistent Organic Pollutant ({POP}) as it persists in the environment, bio-accumulates through the food web, causes adverse effects to human health and the environment and transports across international boundaries far from its sources. The atypical physico-chemical properties of chlordecone make its inclusion in classical analytical approaches non applicable. The aim of our work was to include chlordecone in a multi organochlorine residue method preventing any degradation during the analytical process and thus allowing quantification at ppt (ng kg−1 or ng L−1) levels for a wide range of {OCPs} in breast milk, human serum and adipose tissue. After {GC}–{HRMS} vs. {MS}/{MS} and {EI} vs. {APCI} comparisons, the major improvement in terms of sensitivity was found in decreasing the length and film thickness of the gas chromatography column. Thanks to a linear correlation between relative response and quantity of chlordecone injected, {LC}-({ESI}-)-{MS}/{MS} was finally preferred. An acetonitrile based gradient optimized on a C30 coreshell {HPLC} column has led to reaching limits of quantification as low as 8 ng L−1, 25 pg {mL}−1 and 0.2 ng g−1 fat for breast milk, serum and adipose tissue, respectively, allowing multiresidue {OCP} quantification at concentration levels compatible with biomonitoring purposes and pre-requisites.},
	pages = {169--177},
	journaltitle = {Journal of Chromatography A},
	author = {Bichon, Emmanuelle and Guiffard, Ingrid and Vénisseau, Anaïs and Marchand, Philippe and Antignac, Jean-Philippe and Le Bizec, Bruno},
	urldate = {2019-03-29},
	date = {2015-08},
	langid = {english},
	file = {Bichon et al. - 2015 - Ultra-trace quantification method for chlordecone .pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\7L9SQVGY\\Bichon et al. - 2015 - Ultra-trace quantification method for chlordecone .pdf:application/pdf}
}
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