Determination of the amino acid sequence in oligopeptides by computer interpretation of their high-resolution mass spectra. Biemann, K., Cone, C., Webster, B. R., & Arsenault, G. P. J Am Chem Soc, 88(23):5598–5606, 1966. abstract bibtex A technique for the amino acid sequence determination of oligopeptides based on the computer interpretation of the high resolution mass spectra of certain peptide derivatives is described. It has been successfully applied to di- through pentapeptides containing glycine, alanine, serine, proline, valine, threonine, leucine, asparagine, a-aminoadipic acid, lysine, glutamic acid, methionine, histidine, phenylalanine, tyrosine, and S-benzylcysteine and with acetyl, trifluoroacetyl, chlorodifluoroacetyl, benzoyl, carbobenzoxy, phthaloyl, chlorophthaloyl, tosyl, and 3-hydroxydodecanoyl as N-terminal substituents. Because of its speed, sensitivity (a few micrograms yield a usable spectrum), relative tolerance toward contaminants, and objectivity of interpretation, the technique should prove useful in the course of the many sequence determinations required for the determination of the structure of a protein.
@Article{biemann66determination,
author = {K. Biemann and C. Cone and B. R. Webster and G. P. Arsenault},
title = {Determination of the amino acid sequence in oligopeptides by computer interpretation of their high-resolution mass spectra.},
journal = {J Am Chem Soc},
year = {1966},
volume = {88},
number = {23},
pages = {5598--5606},
abstract = {A technique for the amino acid sequence determination of oligopeptides based on the computer interpretation of the high resolution mass spectra of certain peptide derivatives is described. It has been successfully applied to di- through pentapeptides containing glycine, alanine, serine, proline, valine, threonine, leucine, asparagine, a-aminoadipic acid, lysine, glutamic acid, methionine, histidine, phenylalanine, tyrosine, and S-benzylcysteine and with acetyl, trifluoroacetyl, chlorodifluoroacetyl, benzoyl, carbobenzoxy, phthaloyl, chlorophthaloyl, tosyl, and 3-hydroxydodecanoyl as N-terminal substituents. Because of its speed, sensitivity (a few micrograms yield a usable spectrum), relative tolerance toward contaminants, and objectivity of interpretation, the technique should prove useful in the course of the many sequence determinations required for the determination of the structure of a protein.},
keywords = {Mass Spectrometry, MS, peptide sequencing, peptide de novo sequencing},
owner = {Sebastian},
pmid = {5980176},
timestamp = {2010.11.05},
}
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