Quantitative visualization of DNA G-quadruplex structures in human cells. Biffi, G., Tannahill, D., McCafferty, J., & Balasubramanian, S. Nature chemistry, 5(3):182–6, March, 2013. ![Quantitative visualization of DNA G-quadruplex structures in human cells. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function.
@article{Biffi2013,
title = {Quantitative visualization of {DNA} {G}-quadruplex structures in human cells.},
volume = {5},
issn = {1755-4349},
url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3622242&tool=pmcentrez&rendertype=abstract},
doi = {10.1038/nchem.1548},
abstract = {Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function.},
number = {3},
journal = {Nature chemistry},
author = {Biffi, Giulia and Tannahill, David and McCafferty, John and Balasubramanian, Shankar},
month = mar,
year = {2013},
pmid = {23422559},
keywords = {\#nosource, Animals, Bone Neoplasms, Bone Neoplasms: genetics, Cell Cycle, Cell Line, DNA, DNA: chemistry, Enzyme-Linked Immunosorbent Assay, G-Quadruplexes, Humans, Male, Osteosarcoma, Osteosarcoma: genetics, Salmon, Spermatozoa, Spermatozoa: chemistry, Thermodynamics, Tumor},
pages = {182--6},
}
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