Ectopic expression of pectate lyase <i>Ptxt</i>PL1-27 in aspen affects leaf cuticle development. Biswal, A. K., Banasiak, A., Fernández-Moreno, J., Mitra, M., Harholt, J., Derba-Maceluch, M., Majda, M., Kushwah, S., Kumar, V., Abreu, I., Sivan, P., Pattathil, S., Immerzeel, P., Gorzsás, A., Moritz, T., Hahn, M. G., Scheller, H. V., Aharoni, A., & Mellerowicz, E. J. iScience, 28(12):113963, December, 2025. ![Ectopic expression of pectate lyase <i>Ptxt</i>PL1-27 in aspen affects leaf cuticle development [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Cuticle - a hydrophobic barrier of cutin and waxes covering the outer cell wall surface of plants - enables survival in terrestrial habitats. However, it is not understood how the hydrophobic cuticle precursors travel through the homogalacturonan-rich hydrophilic cell wall. To elucidate the role of homogalacturonan in cuticle development, we disrupted its integrity by overexpressing a pectate lyase, PtxtPL1-27, in aspen. PtxtPL1-27 had pleiotropic effects on shoot development, including the reduction of cuticle thickness and changes in cutin and wax composition, but the expression of cutin biosynthetic genes was little affected. Despite a reduction in homogalacturonan content in the leaves, labeling with the homogalacturonan-specific antibody JIM5 in the outer epidermal cell wall layer increased and displayed an altered pattern. Moreover, the ultrastructure of cell walls was changed concomitant with lipid accumulation. We propose that the disruption of homogalacturonan integrity affected the cutinsome-dependent transport and polymerization of cutin monomers in the cell wall.
@article{biswal_ectopic_2025,
title = {Ectopic expression of pectate lyase \textit{{Ptxt}}{PL1}-27 in aspen affects leaf cuticle development},
volume = {28},
issn = {2589-0042},
url = {https://www.sciencedirect.com/science/article/pii/S2589004225022242},
doi = {10.1016/j.isci.2025.113963},
abstract = {Cuticle - a hydrophobic barrier of cutin and waxes covering the outer cell wall surface of plants - enables survival in terrestrial habitats. However, it is not understood how the hydrophobic cuticle precursors travel through the homogalacturonan-rich hydrophilic cell wall. To elucidate the role of homogalacturonan in cuticle development, we disrupted its integrity by overexpressing a pectate lyase, PtxtPL1-27, in aspen. PtxtPL1-27 had pleiotropic effects on shoot development, including the reduction of cuticle thickness and changes in cutin and wax composition, but the expression of cutin biosynthetic genes was little affected. Despite a reduction in homogalacturonan content in the leaves, labeling with the homogalacturonan-specific antibody JIM5 in the outer epidermal cell wall layer increased and displayed an altered pattern. Moreover, the ultrastructure of cell walls was changed concomitant with lipid accumulation. We propose that the disruption of homogalacturonan integrity affected the cutinsome-dependent transport and polymerization of cutin monomers in the cell wall.},
number = {12},
urldate = {2025-11-28},
journal = {iScience},
author = {Biswal, Ajaya K. and Banasiak, Alicja and Fernández-Moreno, Josefina-Patricia and Mitra, Madhusree and Harholt, Jesper and Derba-Maceluch, Marta and Majda, Mateusz and Kushwah, Sunita and Kumar, Vikash and Abreu, Ilka and Sivan, Pramod and Pattathil, Sivakumar and Immerzeel, Peter and Gorzsás, András and Moritz, Thomas and Hahn, Michael G. and Scheller, Henrik Vibe and Aharoni, Asaph and Mellerowicz, Ewa J.},
month = dec,
year = {2025},
keywords = {Plant Biology, Plant anatomy, Plant development, Plant physiology},
pages = {113963},
}
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However, it is not understood how the hydrophobic cuticle precursors travel through the homogalacturonan-rich hydrophilic cell wall. To elucidate the role of homogalacturonan in cuticle development, we disrupted its integrity by overexpressing a pectate lyase, PtxtPL1-27, in aspen. PtxtPL1-27 had pleiotropic effects on shoot development, including the reduction of cuticle thickness and changes in cutin and wax composition, but the expression of cutin biosynthetic genes was little affected. Despite a reduction in homogalacturonan content in the leaves, labeling with the homogalacturonan-specific antibody JIM5 in the outer epidermal cell wall layer increased and displayed an altered pattern. Moreover, the ultrastructure of cell walls was changed concomitant with lipid accumulation. 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However, it is not understood how the hydrophobic cuticle precursors travel through the homogalacturonan-rich hydrophilic cell wall. To elucidate the role of homogalacturonan in cuticle development, we disrupted its integrity by overexpressing a pectate lyase, PtxtPL1-27, in aspen. PtxtPL1-27 had pleiotropic effects on shoot development, including the reduction of cuticle thickness and changes in cutin and wax composition, but the expression of cutin biosynthetic genes was little affected. Despite a reduction in homogalacturonan content in the leaves, labeling with the homogalacturonan-specific antibody JIM5 in the outer epidermal cell wall layer increased and displayed an altered pattern. Moreover, the ultrastructure of cell walls was changed concomitant with lipid accumulation. 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