Using a Microcantilever Array for Detecting Phase Transitions and Stability of DNA. Biswal, S. L., Raorane, D., Chaiken, A., & Majumdar, A. Journal of the Association for Laboratory Automation, 11:222-226, 2006-08-01, 2006. ![Using a Microcantilever Array for Detecting Phase Transitions and Stability of DNA [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex We report the extension of the microcantilever platform to study the thermal phase transition of biomolecules as they are heated. Microcantilever-based sensors directly translate changes in Gibbs free energy due to macromolecular interactions into mechanical responses. We observe surface stress changes in response to thermal dehybridization of double-stranded DNA oligonucleotides that are attached onto one side of a microcantilever. Once the cantilever is heated, the DNA undergoes a transition as the complementary strand melts, which results in changes in the cantilever deflection. This deflection is due to changes in the electrostatic, ionic, and hydration interaction forces between the remaining immobilized DNA strands. This new technique has allowed us to probe DNA melting dynamics and leads to a better understanding of the stability of DNA complexes on surfaces.
@article {635,
title = {Using a Microcantilever Array for Detecting Phase Transitions and Stability of DNA},
journal = {Journal of the Association for Laboratory Automation},
volume = {11},
year = {2006},
month = {2006-08-01},
pages = {222-226},
abstract = {We report the extension of the microcantilever platform to study the thermal phase transition of biomolecules as they are heated. Microcantilever-based sensors directly translate changes in Gibbs free energy due to macromolecular interactions into mechanical responses. We observe surface stress changes in response to thermal dehybridization of double-stranded DNA oligonucleotides that are attached onto one side of a microcantilever. Once the cantilever is heated, the DNA undergoes a transition as the complementary strand melts, which results in changes in the cantilever deflection. This deflection is due to changes in the electrostatic, ionic, and hydration interaction forces between the remaining immobilized DNA strands. This new technique has allowed us to probe DNA melting dynamics and leads to a better understanding of the stability of DNA complexes on surfaces.},
keywords = {DNA melting, microcantilevers, thermal denaturation},
isbn = {2211-0682, 1540-2452},
url = {http://jla.sagepub.com/content/11/4/222},
author = {Biswal, Sibani Lisa and Raorane, Digvijay and Chaiken, Alison and Majumdar, Arun}
}
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This deflection is due to changes in the electrostatic, ionic, and hydration interaction forces between the remaining immobilized DNA strands. This new technique has allowed us to probe DNA melting dynamics and leads to a better understanding of the stability of DNA complexes on surfaces.","keywords":"DNA melting, microcantilevers, thermal denaturation","isbn":"2211-0682, 1540-2452","url":"http://jla.sagepub.com/content/11/4/222","author":[{"propositions":[],"lastnames":["Biswal"],"firstnames":["Sibani","Lisa"],"suffixes":[]},{"propositions":[],"lastnames":["Raorane"],"firstnames":["Digvijay"],"suffixes":[]},{"propositions":[],"lastnames":["Chaiken"],"firstnames":["Alison"],"suffixes":[]},{"propositions":[],"lastnames":["Majumdar"],"firstnames":["Arun"],"suffixes":[]}],"bibtex":"@article {635,\n\ttitle = {Using a Microcantilever Array for Detecting Phase Transitions and Stability of DNA},\n\tjournal = {Journal of the Association for Laboratory Automation},\n\tvolume = {11},\n\tyear = {2006},\n\tmonth = {2006-08-01},\n\tpages = {222-226},\n\tabstract = {We report the extension of the microcantilever platform to study the thermal phase transition of biomolecules as they are heated. Microcantilever-based sensors directly translate changes in Gibbs free energy due to macromolecular interactions into mechanical responses. We observe surface stress changes in response to thermal dehybridization of double-stranded DNA oligonucleotides that are attached onto one side of a microcantilever. Once the cantilever is heated, the DNA undergoes a transition as the complementary strand melts, which results in changes in the cantilever deflection. This deflection is due to changes in the electrostatic, ionic, and hydration interaction forces between the remaining immobilized DNA strands. This new technique has allowed us to probe DNA melting dynamics and leads to a better understanding of the stability of DNA complexes on surfaces.},\n\tkeywords = {DNA melting, microcantilevers, thermal denaturation},\n\tisbn = {2211-0682, 1540-2452},\n\turl = {http://jla.sagepub.com/content/11/4/222},\n\tauthor = {Biswal, Sibani Lisa and Raorane, Digvijay and Chaiken, Alison and Majumdar, Arun}\n}\n","author_short":["Biswal, S. 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