@ARTICLE{Bittman1997-ow,
  title    = "Cell coupling and uncoupling in the ventricular zone of
              developing neocortex",
  author   = "Bittman, K and Owens, D F and Kriegstein, A R and LoTurco, J J",
  abstract = "Cells within the ventricular zone (VZ) of developing neocortex
              are coupled together into clusters by gap junction channels. The
              specific role of clustering in cortical neurogenesis is unknown;
              however, clustering provides a means for spatially restricted
              local interactions between subsets of precursors and other cells
              within the VZ. In the present study, we have used a combination
              of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular
              biocytin labeling, and immunocytochemistry to determine when in
              the cell cycle VZ cells couple and uncouple from clusters and to
              determine what cell types within the VZ are coupled to clusters.
              Our results indicate that clusters contain radial glia and neural
              precursors but do not contain differentiating or migrating
              neurons. In early neurogenesis, all precursors in S and G2 phases
              of the cell cycle are coupled, and approximately half of the
              cells in G1 are coupled. In late neurogenesis, however, over half
              of the cells in both G1 and S phases are not coupled to VZ
              clusters, whereas all cells in G2 are coupled to clusters.
              Increased uncoupling in S phase during late neurogenesis may
              contribute to the greater percentage of VZ cells exiting the cell
              cycle at this time. Consistent with this hypothesis, we found
              that pharmacologically uncoupling VZ cells with octanol decreases
              the percentage of VZ cells that enter S phase. These results
              demonstrate that cell clustering in the VZ is restricted to
              neural precursors and radial glia, is dynamic through the cell
              cycle, and may play a role in regulating neurogenesis.",
  journal  = "J Neurosci",
  volume   =  17,
  number   =  18,
  pages    = "7037--7044",
  month    =  sep,
  year     =  1997,
  language = "en"
}

{"_id":"L7jHjLwkNHW8AwDGR","bibbaseid":"bittman-owens-kriegstein-loturco-cellcouplinganduncouplingintheventricularzoneofdevelopingneocortex-1997","author_short":["Bittman, K","Owens, D F","Kriegstein, A R","LoTurco, J J"],"bibdata":{"bibtype":"article","type":"article","title":"Cell coupling and uncoupling in the ventricular zone of developing neocortex","author":[{"propositions":[],"lastnames":["Bittman"],"firstnames":["K"],"suffixes":[]},{"propositions":[],"lastnames":["Owens"],"firstnames":["D","F"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["A","R"],"suffixes":[]},{"propositions":[],"lastnames":["LoTurco"],"firstnames":["J","J"],"suffixes":[]}],"abstract":"Cells within the ventricular zone (VZ) of developing neocortex are coupled together into clusters by gap junction channels. The specific role of clustering in cortical neurogenesis is unknown; however, clustering provides a means for spatially restricted local interactions between subsets of precursors and other cells within the VZ. In the present study, we have used a combination of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular biocytin labeling, and immunocytochemistry to determine when in the cell cycle VZ cells couple and uncouple from clusters and to determine what cell types within the VZ are coupled to clusters. Our results indicate that clusters contain radial glia and neural precursors but do not contain differentiating or migrating neurons. In early neurogenesis, all precursors in S and G2 phases of the cell cycle are coupled, and approximately half of the cells in G1 are coupled. In late neurogenesis, however, over half of the cells in both G1 and S phases are not coupled to VZ clusters, whereas all cells in G2 are coupled to clusters. Increased uncoupling in S phase during late neurogenesis may contribute to the greater percentage of VZ cells exiting the cell cycle at this time. Consistent with this hypothesis, we found that pharmacologically uncoupling VZ cells with octanol decreases the percentage of VZ cells that enter S phase. These results demonstrate that cell clustering in the VZ is restricted to neural precursors and radial glia, is dynamic through the cell cycle, and may play a role in regulating neurogenesis.","journal":"J Neurosci","volume":"17","number":"18","pages":"7037–7044","month":"September","year":"1997","language":"en","bibtex":"@ARTICLE{Bittman1997-ow,\n title = \"Cell coupling and uncoupling in the ventricular zone of\n developing neocortex\",\n author = \"Bittman, K and Owens, D F and Kriegstein, A R and LoTurco, J J\",\n abstract = \"Cells within the ventricular zone (VZ) of developing neocortex\n are coupled together into clusters by gap junction channels. The\n specific role of clustering in cortical neurogenesis is unknown;\n however, clustering provides a means for spatially restricted\n local interactions between subsets of precursors and other cells\n within the VZ. In the present study, we have used a combination\n of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular\n biocytin labeling, and immunocytochemistry to determine when in\n the cell cycle VZ cells couple and uncouple from clusters and to\n determine what cell types within the VZ are coupled to clusters.\n Our results indicate that clusters contain radial glia and neural\n precursors but do not contain differentiating or migrating\n neurons. In early neurogenesis, all precursors in S and G2 phases\n of the cell cycle are coupled, and approximately half of the\n cells in G1 are coupled. In late neurogenesis, however, over half\n of the cells in both G1 and S phases are not coupled to VZ\n clusters, whereas all cells in G2 are coupled to clusters.\n Increased uncoupling in S phase during late neurogenesis may\n contribute to the greater percentage of VZ cells exiting the cell\n cycle at this time. Consistent with this hypothesis, we found\n that pharmacologically uncoupling VZ cells with octanol decreases\n the percentage of VZ cells that enter S phase. These results\n demonstrate that cell clustering in the VZ is restricted to\n neural precursors and radial glia, is dynamic through the cell\n cycle, and may play a role in regulating neurogenesis.\",\n journal = \"J Neurosci\",\n volume = 17,\n number = 18,\n pages = \"7037--7044\",\n month = sep,\n year = 1997,\n language = \"en\"\n}\n\n","author_short":["Bittman, K","Owens, D F","Kriegstein, A R","LoTurco, J J"],"key":"Bittman1997-ow","id":"Bittman1997-ow","bibbaseid":"bittman-owens-kriegstein-loturco-cellcouplinganduncouplingintheventricularzoneofdevelopingneocortex-1997","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["cell","coupling","uncoupling","ventricular","zone","developing","neocortex","bittman","owens","kriegstein","loturco"],"title":"Cell coupling and uncoupling in the ventricular zone of developing neocortex","year":1997}