Elucidation of a novel lacto-N-decaose core structure in human milk using nonlinear analytical technique combinations. Blank, D., Geyer, H., Maass, K., Yamashita, K., & Geyer, R. Anal Biochem, 421(2):680–690, 2012. doi abstract bibtex Detailed structural analysis of high molecular weight human milk oligosaccharides (HMOs) is still a challenging task. Here we present a modular strategy for a flexible de novo structural characterization of this class of molecules. The protocol combines established techniques such as separation by two-dimensional high-performance liquid chromatography with different types of mass spectrometry, exoglycosidase digestion, and linkage analysis in an individual glycan-based manner. As a proof of principle, this approach was applied to two distinct HMO isomers representing a difucosylated octaose core and a trifucosylated decaose core. Obtained data revealed the presence of one terminal Lewis A and one internal Lewis X epitope in the case of the octaose and led to the identification of this molecule as a difucosylated iso-lacto-N-octaose. The trifucosylated, doubly branched lacto-N-neo-decaose was shown to represent a new type of HMO core structure in which the branched antenna is linked to carbon atom 3 of the innermost galactosyl residue. Hence, using this analytical protocol a novel HMO structure could be defined. Our results further demonstrate that a combination of different techniques may be required for de novo structural analysis of these molecules.
@Article{blank12elucidation,
author = {Dennis Blank and Hildegard Geyer and Kai Maass and Katsuko Yamashita and Rudolf Geyer},
title = {Elucidation of a novel lacto-{N}-decaose core structure in human milk using nonlinear analytical technique combinations.},
journal = {Anal Biochem},
year = {2012},
volume = {421},
number = {2},
pages = {680--690},
abstract = {Detailed structural analysis of high molecular weight human milk oligosaccharides (HMOs) is still a challenging task. Here we present a modular strategy for a flexible de novo structural characterization of this class of molecules. The protocol combines established techniques such as separation by two-dimensional high-performance liquid chromatography with different types of mass spectrometry, exoglycosidase digestion, and linkage analysis in an individual glycan-based manner. As a proof of principle, this approach was applied to two distinct HMO isomers representing a difucosylated octaose core and a trifucosylated decaose core. Obtained data revealed the presence of one terminal Lewis A and one internal Lewis X epitope in the case of the octaose and led to the identification of this molecule as a difucosylated iso-lacto-N-octaose. The trifucosylated, doubly branched lacto-N-neo-decaose was shown to represent a new type of HMO core structure in which the branched antenna is linked to carbon atom 3 of the innermost galactosyl residue. Hence, using this analytical protocol a novel HMO structure could be defined. Our results further demonstrate that a combination of different techniques may be required for de novo structural analysis of these molecules.},
doi = {10.1016/j.ab.2011.11.030},
keywords = {glycans; glycan sequencing; glycan MS; mass spectrometry},
owner = {Sebastian},
pmid = {22197416},
timestamp = {2012.04.25},
}
Downloads: 0
{"_id":"X4JCbgCDvj78ZLqSE","bibbaseid":"blank-geyer-maass-yamashita-geyer-elucidationofanovellactondecaosecorestructureinhumanmilkusingnonlinearanalyticaltechniquecombinations-2012","authorIDs":[],"author_short":["Blank, D.","Geyer, H.","Maass, K.","Yamashita, K.","Geyer, R."],"bibdata":{"bibtype":"article","type":"article","author":[{"firstnames":["Dennis"],"propositions":[],"lastnames":["Blank"],"suffixes":[]},{"firstnames":["Hildegard"],"propositions":[],"lastnames":["Geyer"],"suffixes":[]},{"firstnames":["Kai"],"propositions":[],"lastnames":["Maass"],"suffixes":[]},{"firstnames":["Katsuko"],"propositions":[],"lastnames":["Yamashita"],"suffixes":[]},{"firstnames":["Rudolf"],"propositions":[],"lastnames":["Geyer"],"suffixes":[]}],"title":"Elucidation of a novel lacto-N-decaose core structure in human milk using nonlinear analytical technique combinations.","journal":"Anal Biochem","year":"2012","volume":"421","number":"2","pages":"680–690","abstract":"Detailed structural analysis of high molecular weight human milk oligosaccharides (HMOs) is still a challenging task. Here we present a modular strategy for a flexible de novo structural characterization of this class of molecules. The protocol combines established techniques such as separation by two-dimensional high-performance liquid chromatography with different types of mass spectrometry, exoglycosidase digestion, and linkage analysis in an individual glycan-based manner. As a proof of principle, this approach was applied to two distinct HMO isomers representing a difucosylated octaose core and a trifucosylated decaose core. Obtained data revealed the presence of one terminal Lewis A and one internal Lewis X epitope in the case of the octaose and led to the identification of this molecule as a difucosylated iso-lacto-N-octaose. The trifucosylated, doubly branched lacto-N-neo-decaose was shown to represent a new type of HMO core structure in which the branched antenna is linked to carbon atom 3 of the innermost galactosyl residue. Hence, using this analytical protocol a novel HMO structure could be defined. Our results further demonstrate that a combination of different techniques may be required for de novo structural analysis of these molecules.","doi":"10.1016/j.ab.2011.11.030","keywords":"glycans; glycan sequencing; glycan MS; mass spectrometry","owner":"Sebastian","pmid":"22197416","timestamp":"2012.04.25","bibtex":"@Article{blank12elucidation,\n author = {Dennis Blank and Hildegard Geyer and Kai Maass and Katsuko Yamashita and Rudolf Geyer},\n title = {Elucidation of a novel lacto-{N}-decaose core structure in human milk using nonlinear analytical technique combinations.},\n journal = {Anal Biochem},\n year = {2012},\n volume = {421},\n number = {2},\n pages = {680--690},\n abstract = {Detailed structural analysis of high molecular weight human milk oligosaccharides (HMOs) is still a challenging task. Here we present a modular strategy for a flexible de novo structural characterization of this class of molecules. The protocol combines established techniques such as separation by two-dimensional high-performance liquid chromatography with different types of mass spectrometry, exoglycosidase digestion, and linkage analysis in an individual glycan-based manner. As a proof of principle, this approach was applied to two distinct HMO isomers representing a difucosylated octaose core and a trifucosylated decaose core. Obtained data revealed the presence of one terminal Lewis A and one internal Lewis X epitope in the case of the octaose and led to the identification of this molecule as a difucosylated iso-lacto-N-octaose. The trifucosylated, doubly branched lacto-N-neo-decaose was shown to represent a new type of HMO core structure in which the branched antenna is linked to carbon atom 3 of the innermost galactosyl residue. Hence, using this analytical protocol a novel HMO structure could be defined. Our results further demonstrate that a combination of different techniques may be required for de novo structural analysis of these molecules.},\n doi = {10.1016/j.ab.2011.11.030},\n keywords = {glycans; glycan sequencing; glycan MS; mass spectrometry},\n owner = {Sebastian},\n pmid = {22197416},\n timestamp = {2012.04.25},\n}\n\n","author_short":["Blank, D.","Geyer, H.","Maass, K.","Yamashita, K.","Geyer, R."],"key":"blank12elucidation","id":"blank12elucidation","bibbaseid":"blank-geyer-maass-yamashita-geyer-elucidationofanovellactondecaosecorestructureinhumanmilkusingnonlinearanalyticaltechniquecombinations-2012","role":"author","urls":{},"keyword":["glycans; glycan sequencing; glycan MS; mass spectrometry"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://git.bio.informatik.uni-jena.de/fleisch/literature/raw/master/group-literature.bib","creationDate":"2019-11-19T16:50:41.624Z","downloads":0,"keywords":["glycans; glycan sequencing; glycan ms; mass spectrometry"],"search_terms":["elucidation","novel","lacto","decaose","core","structure","human","milk","using","nonlinear","analytical","technique","combinations","blank","geyer","maass","yamashita","geyer"],"title":"Elucidation of a novel lacto-N-decaose core structure in human milk using nonlinear analytical technique combinations.","year":2012,"dataSources":["C5FtkvWWggFfMJTFX"]}