Molecular Cytogenetics and Flow Cytometry Reveal Conserved Genome Organization in Pinus Mugo and P. Uncinata

Molecular Cytogenetics and Flow Cytometry Reveal Conserved Genome Organization in Pinus Mugo and P. Uncinata. Bogunić, F., Siljak-Yakovlev, S., Muratović, E., Pustahija, F., & Medjedović, S. 68(1):179–187.

Paper doi abstract bibtex

• Introduction The most common representatives of the European mountain pine complex (Pinus mugo s.l.) are P. mugo s.s. and Pinus uncinata. • Materials and methods Genome characterization of P. mugo and P. uncinata was studied using fluorescence in situ hybridization of 5S and 18-5.8-26S rDNA, fluorochrome banding for heterochromatin characterization, and flow cytometry for DNA content measurement. • Results and discussion Distribution of 5S and 18S rDNA showed identical patterns for both pine species. In contrast, heterochromatin patterns revealed slight differences in the number and position of bands between these two pines. Genome size analysis of 21 P. mugo populations and one P. uncinata population revealed no significant variations across seven European countries. The mean genome size (2C DNA) for the 21 P. mugo populations was 42.56\,±\,0.79 pg, equivalent to 41.62\,×\,103 Mbp, and ranged from 41.08 to 43.95 pg. No relationships were observed between nuclear DNA content and geographic origin of the studied populations. • Conclusions Our results reveal that the mechanisms shaping molecular cytogenetic organization and genome size did not profoundly differentiate the genomes of P. mugo and P. uncinata. Observed variations in heterochromatin patterns indicate ongoing divergence processes in the genomes of the two pines.

@article{bogunicMolecularCytogeneticsFlow2011,
  title = {Molecular Cytogenetics and Flow Cytometry Reveal Conserved Genome Organization in {{Pinus}} Mugo and {{P}}. Uncinata},
  author = {Bogunić, Faruk and Siljak-Yakovlev, Sonja and Muratović, Edina and Pustahija, Fatima and Medjedović, Safer},
  date = {2011},
  journaltitle = {Annals of Forest Science},
  volume = {68},
  pages = {179--187},
  doi = {10.1007/s13595-011-0019-9},
  url = {https://doi.org/10.1007/s13595-011-0019-9},
  abstract = {• Introduction

The most common representatives of the European mountain pine complex (Pinus mugo s.l.) are P. mugo s.s. and Pinus uncinata. • Materials and methods

Genome characterization of P. mugo and P. uncinata was studied using fluorescence in situ hybridization of 5S and 18-5.8-26S rDNA, fluorochrome banding for heterochromatin characterization, and flow cytometry for DNA content measurement. • Results and discussion

Distribution of 5S and 18S rDNA showed identical patterns for both pine species. In contrast, heterochromatin patterns revealed slight differences in the number and position of bands between these two pines. Genome size analysis of 21 P. mugo populations and one P. uncinata population revealed no significant variations across seven European countries. The mean genome size (2C DNA) for the 21 P. mugo populations was 42.56\,±\,0.79 pg, equivalent to 41.62\,×\,103 Mbp, and ranged from 41.08 to 43.95 pg. No relationships were observed between nuclear DNA content and geographic origin of the studied populations. • Conclusions

Our results reveal that the mechanisms shaping molecular cytogenetic organization and genome size did not profoundly differentiate the genomes of P. mugo and P. uncinata. Observed variations in heterochromatin patterns indicate ongoing divergence processes in the genomes of the two pines.},
  keywords = {*imported-from-citeulike-INRMM,~INRMM-MiD:c-13778821,forest-resources,phylogenetics,pinus-mugo,pinus-uncinata,taxonomy},
  number = {1}
}

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