Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel. Bonato, S.; Breton, E.; Didry, M.; Lizon, F.; Cornille, V.; Lécuyer, E.; Christaki, U.; and Artigas, L. F. 156:76–85.
Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel [link]Paper  doi  abstract   bibtex   
A pulse-shape recording flow cytometer (CytoSense©) was applied to the monitoring of changes in phytoplankton distribution along an inshore–offshore transect across the eastern English Channel (EEC), on 13 occasions during the main productive period of the year. Amongst the eight phytoplankton groups discriminated, picophytoplankton (picoeukaryotes and Synechococcus spp.) and Phaeocystis globosa nanoflagellates were the main contributors to total phytoplankton abundance, while Diatoms-like, Coccolithophores, and Cryptophytes represented each one less than 5%. High spatial resolution revealed important changes on relatively short distances. Moreover, a general decrease of Diatoms-like, P. globosa haploid cells, Coccolithophores, and picoeukaryote abundance was evidenced from inshore to offshore waters, associated with an increase of Synechococcus spp. abundance. Seasonal variability accounted for 71% of phytoplankton abundance changes. Compared to previous studies in the area the CytoSense allowed highlighting new players during the winter–spring–summer phytoplankton succession: (i) high abundance of Synechococcus spp. and picoeukaryotes I in winter and of Synechococcus spp. also in the summer, (ii) a transient abundance peak of picoeukaryotes II, and (iii) high abundance of Coccolithophores and Cryptophytes during the wax of P. globosa bloom and in the summer. The relationships between environmental variables and phytoplankton assemblages indicated that nutrients and the daily light intensity were the most important parameters in structuring the winter–spring–summer transitions.
@article{bonato_spatio-temporal_2016,
	title = {Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel},
	volume = {156},
	issn = {0924-7963},
	url = {http://www.sciencedirect.com/science/article/pii/S0924796315002250},
	doi = {10.1016/j.jmarsys.2015.11.009},
	shorttitle = {Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry},
	abstract = {A pulse-shape recording flow cytometer ({CytoSense}©) was applied to the monitoring of changes in phytoplankton distribution along an inshore–offshore transect across the eastern English Channel ({EEC}), on 13 occasions during the main productive period of the year. Amongst the eight phytoplankton groups discriminated, picophytoplankton (picoeukaryotes and Synechococcus spp.) and Phaeocystis globosa nanoflagellates were the main contributors to total phytoplankton abundance, while Diatoms-like, Coccolithophores, and Cryptophytes represented each one less than 5\%. High spatial resolution revealed important changes on relatively short distances. Moreover, a general decrease of Diatoms-like, P. globosa haploid cells, Coccolithophores, and picoeukaryote abundance was evidenced from inshore to offshore waters, associated with an increase of Synechococcus spp. abundance. Seasonal variability accounted for 71\% of phytoplankton abundance changes. Compared to previous studies in the area the {CytoSense} allowed highlighting new players during the winter–spring–summer phytoplankton succession: (i) high abundance of Synechococcus spp. and picoeukaryotes I in winter and of Synechococcus spp. also in the summer, (ii) a transient abundance peak of picoeukaryotes {II}, and (iii) high abundance of Coccolithophores and Cryptophytes during the wax of P. globosa bloom and in the summer. The relationships between environmental variables and phytoplankton assemblages indicated that nutrients and the daily light intensity were the most important parameters in structuring the winter–spring–summer transitions.},
	pages = {76--85},
	journaltitle = {Journal of Marine Systems},
	shortjournal = {Journal of Marine Systems},
	author = {Bonato, Simon and Breton, Elsa and Didry, Morgane and Lizon, Fabrice and Cornille, Vincent and Lécuyer, Eric and Christaki, Urania and Artigas, Luis Felipe},
	urldate = {2019-04-15},
	date = {2016-04-01},
	keywords = {Flow cytometry, Pico-, nano-, and micro-phytoplankton, Seasonal and spatial variability}
}
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