Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation [Izuchenie konformatsionnoǐ situatsii v vodnykh rastvorakh spin-mechenoǐ tRNA-Phe iz Escherichia coli bezomduliatsionnym metodom EPR.]. Bondarev, G., Isaev-Ivanov, V., Isaeva-Ivanova, L., Kirillov, S., & Kleǐner, A. Molekulyarnaya Biologiya, 16(2):352-362, 1982. cited By 1
Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation [Izuchenie konformatsionnoǐ situatsii v vodnykh rastvorakh spin-mechenoǐ tRNA-Phe iz Escherichia coli bezomduliatsionnym metodom EPR.] [link]Paper  abstract   bibtex   
ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solution always exists as a mixture of at least two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and the biological state of tRNA (deacylated, aminoacyl- or peptidyl-tRNA). There are no large structural rearrangements upon aminoacylation or peptidylation of tRNA, the observed small changes of spectral line shape are due to the changes in conformational equilibria.
@ARTICLE{Bondarev1982352,
author={Bondarev, G.N. and Isaev-Ivanov, V.V. and Isaeva-Ivanova, L.S. and Kirillov, S.V. and Kleǐner, A.R.},
title={Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation [Izuchenie konformatsionnoǐ situatsii v vodnykh rastvorakh spin-mechenoǐ tRNA-Phe iz Escherichia coli bezomduliatsionnym metodom EPR.]},
journal={Molekulyarnaya Biologiya},
year={1982},
volume={16},
number={2},
pages={352-362},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020108887&partnerID=40&md5=737d07719caa6d071f7b656012d89f83},
abstract={ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solution always exists as a mixture of at least two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and the biological state of tRNA (deacylated, aminoacyl- or peptidyl-tRNA). There are no large structural rearrangements upon aminoacylation or peptidylation of tRNA, the observed small changes of spectral line shape are due to the changes in conformational equilibria.},
correspondence_address1={Bondarev, G.N.},
issn={00268984},
pubmed_id={6175894},
language={Russian},
abbrev_source_title={Mol Biol (Mosk)},
document_type={Article},
source={Scopus},
}

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