@ARTICLE{Borisova201543,
author={Borisova, A.S. and Reddy, S.K. and Ivanen, D.R. and Bobrov, K.S. and Eneyskaya, E.V. and Rychkov, G.N. and Sandgren, M. and Stålbrand, H. and Sinnott, M.L. and Kulminskaya, A.A. and Shabalin, K.A.},
title={The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolyses of p-nitrophenyl glycosides},
journal={Carbohydrate Research},
year={2015},
volume={412},
pages={43-49},
doi={10.1016/j.carres.2015.03.021},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929649802&doi=10.1016%2fj.carres.2015.03.021&partnerID=40&md5=f216c9f632b9a6d57c20a434a9dbc525},
affiliation={National Research Center Kurchatov Institute, B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Orlova roscha, 188300, Russian Federation; Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, Uppsala, Sweden; Department of Biochemistry and Structural Biology, Lund University, Lund, S-221 00, Sweden; Department of Chemical Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, United Kingdom; St. Petersburg State Polytechnical University, 29 Politekhnicheskaya st., St. Petersburg, 195251, Russian Federation},
abstract={In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k<inf>cat</inf>, K<inf>m</inf> and product K<inf>I</inf> for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α-d-galactose, affected the reaction significantly. © 2015 Elsevier Ltd. All rights reserved.},
author_keywords={Integrated kinetics;  Mutarotation;  Retaining glycoside hydrolase},
funding_details={Российский Фонд Фундаментальных Исследований (РФФИ)12-08-00813-a},
}

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Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Orlova roscha, 188300, Russian Federation; Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, Uppsala, Sweden; Department of Biochemistry and Structural Biology, Lund University, Lund, S-221 00, Sweden; Department of Chemical Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, United Kingdom; St. Petersburg State Polytechnical University, 29 Politekhnicheskaya st., St. Petersburg, 195251, Russian Federation","abstract":"In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k<inf>cat</inf>, K<inf>m</inf> and product K<inf>I</inf> for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α-d-galactose, affected the reaction significantly. © 2015 Elsevier Ltd. All rights reserved.","author_keywords":"Integrated kinetics; Mutarotation; Retaining glycoside hydrolase","funding_details":"Российский Фонд Фундаментальных Исследований (РФФИ)12-08-00813-a","bibtex":"@ARTICLE{Borisova201543,\r\nauthor={Borisova, A.S. and Reddy, S.K. and Ivanen, D.R. and Bobrov, K.S. and Eneyskaya, E.V. and Rychkov, G.N. and Sandgren, M. and Stålbrand, H. and Sinnott, M.L. and Kulminskaya, A.A. and Shabalin, K.A.},\r\ntitle={The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolyses of p-nitrophenyl glycosides},\r\njournal={Carbohydrate Research},\r\nyear={2015},\r\nvolume={412},\r\npages={43-49},\r\ndoi={10.1016/j.carres.2015.03.021},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929649802&doi=10.1016%2fj.carres.2015.03.021&partnerID=40&md5=f216c9f632b9a6d57c20a434a9dbc525},\r\naffiliation={National Research Center Kurchatov Institute, B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Orlova roscha, 188300, Russian Federation; Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, Uppsala, Sweden; Department of Biochemistry and Structural Biology, Lund University, Lund, S-221 00, Sweden; Department of Chemical Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, United Kingdom; St. Petersburg State Polytechnical University, 29 Politekhnicheskaya st., St. Petersburg, 195251, Russian Federation},\r\nabstract={In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k<inf>cat</inf>, K<inf>m</inf> and product K<inf>I</inf> for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α-d-galactose, affected the reaction significantly. © 2015 Elsevier Ltd. All rights reserved.},\r\nauthor_keywords={Integrated kinetics; Mutarotation; Retaining glycoside hydrolase},\r\nfunding_details={Российский Фонд Фундаментальных Исследований (РФФИ)12-08-00813-a},\r\n}","author_short":["Borisova, A.","Reddy, S.","Ivanen, D.","Bobrov, K.","Eneyskaya, E.","Rychkov, G.","Sandgren, M.","Stålbrand, H.","Sinnott, M.","Kulminskaya, A.","Shabalin, K."],"key":"Borisova201543","id":"Borisova201543","bibbaseid":"borisova-reddy-ivanen-bobrov-eneyskaya-rychkov-sandgren-stlbrand-etal-themethodofintegratedkineticsanditsapplicabilitytotheexoglycosidasecatalyzedhydrolysesofpnitrophenylglycosides-2015","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929649802&doi=10.1016%2fj.carres.2015.03.021&partnerID=40&md5=f216c9f632b9a6d57c20a434a9dbc525"},"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lmkb_2019_10.txt","creationDate":"2019-11-05T14:32:09.805Z","downloads":0,"keywords":[],"search_terms":["method","integrated","kinetics","applicability","exo","glycosidase","catalyzed","hydrolyses","nitrophenyl","glycosides","borisova","reddy","ivanen","bobrov","eneyskaya","rychkov","sandgren","stålbrand","sinnott","kulminskaya","shabalin"],"title":"The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolyses of p-nitrophenyl glycosides","year":2015,"dataSources":["rgjRz8LhiuL9Prc4S","MohP3xiH36sPcgi5S"]}