@article{
 title = {Polynucleobacter necessarius, a model for genome reduction in both free-living and symbiotic bacteria},
 type = {article},
 year = {2013},
 keywords = {Amino Acid Sequence,Bacterial,Base Sequence,Betaproteobacteria,Burkholderiac,Burkholderiales,DNA,Genome,Genome Size,Molecul,Molecular,Molecular Sequence Annotation,Symbiosis,article,ero,fresh water,genome streamlining,nonsynonymo},
 pages = {18590-18595},
 volume = {110},
 websites = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887493719&doi=10.1073%2Fpnas.1316687110&partnerID=40&md5=ed62b2e4db69f701238c434431aebd49},
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 created = {2019-10-01T17:20:34.138Z},
 file_attached = {false},
 profile_id = {42d295c0-0737-38d6-8b43-508cab6ea85d},
 last_modified = {2019-10-01T17:24:47.944Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 citation_key = {Boscaro201318590},
 source_type = {article},
 notes = {cited By 21},
 folder_uuids = {b4e18ac7-7050-4c86-a2a4-a9c35bdc74d4},
 private_publication = {false},
 abstract = {We present the complete genomic sequence of the essential symbiont Polynucleobacter necessarius ( Betaproteobacteria), which is a valuable case study for several reasons. First, it is hosted by a ciliated protist, Euplotes; bacterial symbionts of ciliates are still poorly known because of a lack of extensive molecular data. Second, the single species P. necessarius contains both symbiotic and free-living strains, allowing for a comparison between closely related organisms with different ecologies. Third, free-living P. necessarius strains are exceptional by themselves because of their small genome size, reduced metabolic flexibility, and high worldwide abundance in freshwater systems. We provide a comparative analysis of P. necessarius metabolism and explore the peculiar features of a genome reduction that occurred on an already streamlined genome. We compare this unusual system with current hypotheses for genome erosion in symbionts and free-living bacteria, propose modifications to the presently accepted model, and discuss the potential consequences of translesion DNA polymerase loss.},
 bibtype = {article},
 author = {Boscaro, V and Felletti, M and Vannini, C and Ackerman, M S and Chain, P S G and Malfatti, S and Vergez, L M and Shin, M and Doak, T G and Lynch, M and Petroni, G},
 doi = {10.1073/pnas.1316687110},
 journal = {Proceedings of the National Academy of Sciences of the United States of America},
 number = {46}
}

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