Biosynthesis of soluble carotenoid holoproteins in Escherichia coli. Bourcier de Carbon, C., Thurotte, A., Wilson, A., Perreau, F., & Kirilovsky, D. Scientific Reports, 5:9085, March, 2015. WOS:000351149500016doi abstract bibtex Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries.
@article{bourcier_de_carbon_biosynthesis_2015,
title = {Biosynthesis of soluble carotenoid holoproteins in {Escherichia} coli},
volume = {5},
copyright = {All rights reserved},
issn = {2045-2322},
doi = {10.1038/srep09085},
abstract = {Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries.},
language = {English},
journal = {Scientific Reports},
author = {Bourcier de Carbon, Celine and Thurotte, Adrien and Wilson, Adjele and Perreau, Francois and Kirilovsky, Diana},
month = mar,
year = {2015},
note = {WOS:000351149500016},
keywords = {Antioxidants/metabolism, Carotenoids/*metabolism, Escherichia coli Proteins/biosynthesis/chemistry/g, Escherichia coli/genetics/*metabolism, Models, Molecular, Photochemical Processes, Protein Conformation, Recombinant Fusion Proteins/genetics/metabolism, Zeaxanthin, binding-protein, conversion, cyanobacterial photoprotection, functional-analysis, microorganisms, pcc 6803, photosynthesis, phycobilisome, reconstitution},
pages = {9085},
}
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The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. 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