A microfluidic platform for physical entrapment of yeast cells with continuous production of invertase

A microfluidic platform for physical entrapment of yeast cells with continuous production of invertase. Bras, E. J., Chu, V., Aires-Barros, M. R., Conde, J. P., & Fernandes, P. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 92(2):334-341, FEB, 2017.
doi abstract bibtex

BACKGROUND: The rapidly increasing use of biotechnology in many industries has led to the need for novel methods for cell culture which provide an efficient way to either optimize or perform fermentation operations. In parallel, microfabrication techniques allowed the development of microfluidic chips for complex handling of fluids and cells. RESULTS: This work presents a microfluidic platform to trap non-adherent cells for the continuous production of a biomolecule of interest. The biological system chosen as a model was the yeast species Saccharomyces cerevisiae and the extracellular protein invertase. The use of the appropriate combination of the flow rate of the medium, medium dilution rate, and pH allowed effective control of the cell growth in the microfluidic bioreactor while at the same time maximizing the invertase activity per cell. The microfluidic bioreactor allowed for continuous cell culture for 32 h and its productivity both per cell (3.22x10(-8) U cell(-1)) and per consumed nutrient (3.79 U mg(-1) sucrose) was consistently higher than its macroscale batch and continuous reactor counterparts. CONCLUSION: This work demonstrated that a microfluidic bioreactor can be used for continuous production of an extracellular protein using hydrodynamically trapped non-adherent yeast cells. (C) 2016 Society of Chemical Industry

@article{ ISI:000396893400010,
Author = {Bras, Eduardo Js and Chu, Virginia and Aires-Barros, Maria R. and Conde,
   Joao P. and Fernandes, Pedro},
Title = {{A microfluidic platform for physical entrapment of yeast cells with
   continuous production of invertase}},
Journal = {{JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY}},
Year = {{2017}},
Volume = {{92}},
Number = {{2}},
Pages = {{334-341}},
Month = {{FEB}},
Abstract = {{BACKGROUND: The rapidly increasing use of biotechnology in many
   industries has led to the need for novel methods for cell culture which
   provide an efficient way to either optimize or perform fermentation
   operations. In parallel, microfabrication techniques allowed the
   development of microfluidic chips for complex handling of fluids and
   cells.
   RESULTS: This work presents a microfluidic platform to trap non-adherent
   cells for the continuous production of a biomolecule of interest. The
   biological system chosen as a model was the yeast species Saccharomyces
   cerevisiae and the extracellular protein invertase. The use of the
   appropriate combination of the flow rate of the medium, medium dilution
   rate, and pH allowed effective control of the cell growth in the
   microfluidic bioreactor while at the same time maximizing the invertase
   activity per cell. The microfluidic bioreactor allowed for continuous
   cell culture for 32 h and its productivity both per cell (3.22x10(-8) U
   cell(-1)) and per consumed nutrient (3.79 U mg(-1) sucrose) was
   consistently higher than its macroscale batch and continuous reactor
   counterparts.
   CONCLUSION: This work demonstrated that a microfluidic bioreactor can be
   used for continuous production of an extracellular protein using
   hydrodynamically trapped non-adherent yeast cells. (C) 2016 Society of
   Chemical Industry}},
DOI = {{10.1002/jctb.5010}},
ISSN = {{0268-2575}},
EISSN = {{1097-4660}},
ResearcherID-Numbers = {{Chu, Virginia/I-6048-2014
   Fernandes, Pedro/G-8180-2012
   Conde, Joao Pedro/F-8533-2012
   Bras, Eduardo/I-3337-2019
   Aires-Barros, Maria Raquel/E-6021-2010}},
ORCID-Numbers = {{Chu, Virginia/0000-0002-5306-4409
   Fernandes, Pedro/0000-0003-0271-7796
   Conde, Joao Pedro/0000-0002-5677-3024
   Bras, Eduardo/0000-0001-8928-8431
   Aires-Barros, Maria Raquel/0000-0002-1813-4280}},
Unique-ID = {{ISI:000396893400010}},
}

Downloads: 0

{"_id":"M9PzfpEjYkznwRJHi","bibbaseid":"bras-chu-airesbarros-conde-fernandes-amicrofluidicplatformforphysicalentrapmentofyeastcellswithcontinuousproductionofinvertase-2017","authorIDs":[],"author_short":["Bras, E. J.","Chu, V.","Aires-Barros, M. R.","Conde, J. P.","Fernandes, P."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Bras"],"firstnames":["Eduardo","Js"],"suffixes":[]},{"propositions":[],"lastnames":["Chu"],"firstnames":["Virginia"],"suffixes":[]},{"propositions":[],"lastnames":["Aires-Barros"],"firstnames":["Maria","R."],"suffixes":[]},{"propositions":[],"lastnames":["Conde"],"firstnames":["Joao","P."],"suffixes":[]},{"propositions":[],"lastnames":["Fernandes"],"firstnames":["Pedro"],"suffixes":[]}],"title":"A microfluidic platform for physical entrapment of yeast cells with continuous production of invertase","journal":"JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY","year":"2017","volume":"92","number":"2","pages":"334-341","month":"FEB","abstract":"BACKGROUND: The rapidly increasing use of biotechnology in many industries has led to the need for novel methods for cell culture which provide an efficient way to either optimize or perform fermentation operations. In parallel, microfabrication techniques allowed the development of microfluidic chips for complex handling of fluids and cells. RESULTS: This work presents a microfluidic platform to trap non-adherent cells for the continuous production of a biomolecule of interest. The biological system chosen as a model was the yeast species Saccharomyces cerevisiae and the extracellular protein invertase. The use of the appropriate combination of the flow rate of the medium, medium dilution rate, and pH allowed effective control of the cell growth in the microfluidic bioreactor while at the same time maximizing the invertase activity per cell. The microfluidic bioreactor allowed for continuous cell culture for 32 h and its productivity both per cell (3.22x10(-8) U cell(-1)) and per consumed nutrient (3.79 U mg(-1) sucrose) was consistently higher than its macroscale batch and continuous reactor counterparts. CONCLUSION: This work demonstrated that a microfluidic bioreactor can be used for continuous production of an extracellular protein using hydrodynamically trapped non-adherent yeast cells. (C) 2016 Society of Chemical Industry","doi":"10.1002/jctb.5010","issn":"0268-2575","eissn":"1097-4660","researcherid-numbers":"Chu, Virginia/I-6048-2014 Fernandes, Pedro/G-8180-2012 Conde, Joao Pedro/F-8533-2012 Bras, Eduardo/I-3337-2019 Aires-Barros, Maria Raquel/E-6021-2010","orcid-numbers":"Chu, Virginia/0000-0002-5306-4409 Fernandes, Pedro/0000-0003-0271-7796 Conde, Joao Pedro/0000-0002-5677-3024 Bras, Eduardo/0000-0001-8928-8431 Aires-Barros, Maria Raquel/0000-0002-1813-4280","unique-id":"ISI:000396893400010","bibtex":"@article{ ISI:000396893400010,\nAuthor = {Bras, Eduardo Js and Chu, Virginia and Aires-Barros, Maria R. and Conde,\n Joao P. and Fernandes, Pedro},\nTitle = {{A microfluidic platform for physical entrapment of yeast cells with\n continuous production of invertase}},\nJournal = {{JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY}},\nYear = {{2017}},\nVolume = {{92}},\nNumber = {{2}},\nPages = {{334-341}},\nMonth = {{FEB}},\nAbstract = {{BACKGROUND: The rapidly increasing use of biotechnology in many\n industries has led to the need for novel methods for cell culture which\n provide an efficient way to either optimize or perform fermentation\n operations. In parallel, microfabrication techniques allowed the\n development of microfluidic chips for complex handling of fluids and\n cells.\n RESULTS: This work presents a microfluidic platform to trap non-adherent\n cells for the continuous production of a biomolecule of interest. The\n biological system chosen as a model was the yeast species Saccharomyces\n cerevisiae and the extracellular protein invertase. The use of the\n appropriate combination of the flow rate of the medium, medium dilution\n rate, and pH allowed effective control of the cell growth in the\n microfluidic bioreactor while at the same time maximizing the invertase\n activity per cell. The microfluidic bioreactor allowed for continuous\n cell culture for 32 h and its productivity both per cell (3.22x10(-8) U\n cell(-1)) and per consumed nutrient (3.79 U mg(-1) sucrose) was\n consistently higher than its macroscale batch and continuous reactor\n counterparts.\n CONCLUSION: This work demonstrated that a microfluidic bioreactor can be\n used for continuous production of an extracellular protein using\n hydrodynamically trapped non-adherent yeast cells. (C) 2016 Society of\n Chemical Industry}},\nDOI = {{10.1002/jctb.5010}},\nISSN = {{0268-2575}},\nEISSN = {{1097-4660}},\nResearcherID-Numbers = {{Chu, Virginia/I-6048-2014\n Fernandes, Pedro/G-8180-2012\n Conde, Joao Pedro/F-8533-2012\n Bras, Eduardo/I-3337-2019\n Aires-Barros, Maria Raquel/E-6021-2010}},\nORCID-Numbers = {{Chu, Virginia/0000-0002-5306-4409\n Fernandes, Pedro/0000-0003-0271-7796\n Conde, Joao Pedro/0000-0002-5677-3024\n Bras, Eduardo/0000-0001-8928-8431\n Aires-Barros, Maria Raquel/0000-0002-1813-4280}},\nUnique-ID = {{ISI:000396893400010}},\n}\n\n","author_short":["Bras, E. J.","Chu, V.","Aires-Barros, M. R.","Conde, J. P.","Fernandes, P."],"key":"ISI:000396893400010","id":"ISI:000396893400010","bibbaseid":"bras-chu-airesbarros-conde-fernandes-amicrofluidicplatformforphysicalentrapmentofyeastcellswithcontinuousproductionofinvertase-2017","role":"author","urls":{},"downloads":0},"bibtype":"article","biburl":"http://nanotec.cnr.it/data/nanotec/nanotec-full.bib","creationDate":"2020-04-21T16:04:30.789Z","downloads":0,"keywords":[],"search_terms":["microfluidic","platform","physical","entrapment","yeast","cells","continuous","production","invertase","bras","chu","aires-barros","conde","fernandes"],"title":"A microfluidic platform for physical entrapment of yeast cells with continuous production of invertase","year":2017,"dataSources":["ZTEbWc6bW5f9DTsjF"]}