Microfluidic device for the point of need detection of a pathogen infection biomarker in grapes. Bras, E. J. S., Fortes, A. M., Chu, V., Fernandes, P., & Conde, J. P. ANALYST, 144(16):4871-4879, AUG 21, 2019. doi abstract bibtex Bacterial, fungal and viral infections in plant systems are on the rise, most of which tend to spread quickly amongst crops. These pathogens are also gaining resistance to known treatments, which makes their early detection a priority to avoid extensive loss of crops and the spreading of disease to animal systems. In this work, we propose a microfluidic platform coupled with integrated thin-film silicon photosensors for the detection of pathogen infections in grapes. This detection was achieved by monitoring the concentration of Azelaic Acid (AzA). This small organic acid plays a significant role in the defense mechanism in plant systems. In this platform, the enzyme tyrosinase was immobilized on microbeads inside a microfluidic system. By colorimetric monitoring of the inhibitory effect of AzA on the enzyme tyrosinase in real time, it was possible, in under 10 minutes, to detect different concentrations of AzA in both buffer and spiked solutions of grape juice, in both cases with limits of detection in the 5-10 nM range. In addition, with this microfluidic device, it was possible to clearly distinguish infected from healthy grape samples at three different grape maturation points. Healthy grape samples showed AzA concentrations in the range of 10-20 nM (post-dilution) while infected samples have an estimated increase of AzA of 10-30x, results which were confirmed using HPLC. In both juice and grape samples an integrated sample preparation stage that decreases the phenol content of the solutions was required to achieve fit-for-purpose sensitivities to AzA.
@article{ ISI:000484201600014,
Author = {Bras, Eduardo J. S. and Fortes, Ana Margarida and Chu, Virginia and
Fernandes, Pedro and Conde, Joao Pedro},
Title = {{Microfluidic device for the point of need detection of a pathogen
infection biomarker in grapes}},
Journal = {{ANALYST}},
Year = {{2019}},
Volume = {{144}},
Number = {{16}},
Pages = {{4871-4879}},
Month = {{AUG 21}},
Abstract = {{Bacterial, fungal and viral infections in plant systems are on the rise,
most of which tend to spread quickly amongst crops. These pathogens are
also gaining resistance to known treatments, which makes their early
detection a priority to avoid extensive loss of crops and the spreading
of disease to animal systems. In this work, we propose a microfluidic
platform coupled with integrated thin-film silicon photosensors for the
detection of pathogen infections in grapes. This detection was achieved
by monitoring the concentration of Azelaic Acid (AzA). This small
organic acid plays a significant role in the defense mechanism in plant
systems. In this platform, the enzyme tyrosinase was immobilized on
microbeads inside a microfluidic system. By colorimetric monitoring of
the inhibitory effect of AzA on the enzyme tyrosinase in real time, it
was possible, in under 10 minutes, to detect different concentrations of
AzA in both buffer and spiked solutions of grape juice, in both cases
with limits of detection in the 5-10 nM range. In addition, with this
microfluidic device, it was possible to clearly distinguish infected
from healthy grape samples at three different grape maturation points.
Healthy grape samples showed AzA concentrations in the range of 10-20 nM
(post-dilution) while infected samples have an estimated increase of AzA
of 10-30x, results which were confirmed using HPLC. In both juice and
grape samples an integrated sample preparation stage that decreases the
phenol content of the solutions was required to achieve fit-for-purpose
sensitivities to AzA.}},
DOI = {{10.1039/c9an01002e}},
ISSN = {{0003-2654}},
EISSN = {{1364-5528}},
ResearcherID-Numbers = {{Fernandes, Pedro/G-8180-2012
Bras, Eduardo/I-3337-2019
Conde, Joao Pedro/F-8533-2012
Chu, Virginia/I-6048-2014}},
ORCID-Numbers = {{Fernandes, Pedro/0000-0003-0271-7796
Bras, Eduardo/0000-0001-8928-8431
Conde, Joao Pedro/0000-0002-5677-3024
Fortes, Ana Margarida/0000-0001-7552-0164
Chu, Virginia/0000-0002-5306-4409}},
Unique-ID = {{ISI:000484201600014}},
}
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P."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Bras"],"firstnames":["Eduardo","J.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Fortes"],"firstnames":["Ana","Margarida"],"suffixes":[]},{"propositions":[],"lastnames":["Chu"],"firstnames":["Virginia"],"suffixes":[]},{"propositions":[],"lastnames":["Fernandes"],"firstnames":["Pedro"],"suffixes":[]},{"propositions":[],"lastnames":["Conde"],"firstnames":["Joao","Pedro"],"suffixes":[]}],"title":"Microfluidic device for the point of need detection of a pathogen infection biomarker in grapes","journal":"ANALYST","year":"2019","volume":"144","number":"16","pages":"4871-4879","month":"AUG 21","abstract":"Bacterial, fungal and viral infections in plant systems are on the rise, most of which tend to spread quickly amongst crops. These pathogens are also gaining resistance to known treatments, which makes their early detection a priority to avoid extensive loss of crops and the spreading of disease to animal systems. In this work, we propose a microfluidic platform coupled with integrated thin-film silicon photosensors for the detection of pathogen infections in grapes. This detection was achieved by monitoring the concentration of Azelaic Acid (AzA). This small organic acid plays a significant role in the defense mechanism in plant systems. In this platform, the enzyme tyrosinase was immobilized on microbeads inside a microfluidic system. By colorimetric monitoring of the inhibitory effect of AzA on the enzyme tyrosinase in real time, it was possible, in under 10 minutes, to detect different concentrations of AzA in both buffer and spiked solutions of grape juice, in both cases with limits of detection in the 5-10 nM range. In addition, with this microfluidic device, it was possible to clearly distinguish infected from healthy grape samples at three different grape maturation points. Healthy grape samples showed AzA concentrations in the range of 10-20 nM (post-dilution) while infected samples have an estimated increase of AzA of 10-30x, results which were confirmed using HPLC. In both juice and grape samples an integrated sample preparation stage that decreases the phenol content of the solutions was required to achieve fit-for-purpose sensitivities to AzA.","doi":"10.1039/c9an01002e","issn":"0003-2654","eissn":"1364-5528","researcherid-numbers":"Fernandes, Pedro/G-8180-2012 Bras, Eduardo/I-3337-2019 Conde, Joao Pedro/F-8533-2012 Chu, Virginia/I-6048-2014","orcid-numbers":"Fernandes, Pedro/0000-0003-0271-7796 Bras, Eduardo/0000-0001-8928-8431 Conde, Joao Pedro/0000-0002-5677-3024 Fortes, Ana Margarida/0000-0001-7552-0164 Chu, Virginia/0000-0002-5306-4409","unique-id":"ISI:000484201600014","bibtex":"@article{ ISI:000484201600014,\nAuthor = {Bras, Eduardo J. S. and Fortes, Ana Margarida and Chu, Virginia and\n Fernandes, Pedro and Conde, Joao Pedro},\nTitle = {{Microfluidic device for the point of need detection of a pathogen\n infection biomarker in grapes}},\nJournal = {{ANALYST}},\nYear = {{2019}},\nVolume = {{144}},\nNumber = {{16}},\nPages = {{4871-4879}},\nMonth = {{AUG 21}},\nAbstract = {{Bacterial, fungal and viral infections in plant systems are on the rise,\n most of which tend to spread quickly amongst crops. These pathogens are\n also gaining resistance to known treatments, which makes their early\n detection a priority to avoid extensive loss of crops and the spreading\n of disease to animal systems. In this work, we propose a microfluidic\n platform coupled with integrated thin-film silicon photosensors for the\n detection of pathogen infections in grapes. This detection was achieved\n by monitoring the concentration of Azelaic Acid (AzA). This small\n organic acid plays a significant role in the defense mechanism in plant\n systems. In this platform, the enzyme tyrosinase was immobilized on\n microbeads inside a microfluidic system. By colorimetric monitoring of\n the inhibitory effect of AzA on the enzyme tyrosinase in real time, it\n was possible, in under 10 minutes, to detect different concentrations of\n AzA in both buffer and spiked solutions of grape juice, in both cases\n with limits of detection in the 5-10 nM range. In addition, with this\n microfluidic device, it was possible to clearly distinguish infected\n from healthy grape samples at three different grape maturation points.\n Healthy grape samples showed AzA concentrations in the range of 10-20 nM\n (post-dilution) while infected samples have an estimated increase of AzA\n of 10-30x, results which were confirmed using HPLC. 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