@article{ ISI:000411536400028,
Author = {Bras, Eduardo J. S. and Soares, Ruben R. G. and Azevedo, Ana M. and
   Fernandes, Pedro and Arevalo-Rodriguez, Miguel and Chu, Virginia and
   Conde, Joao P. and Raquel Aires-Barros, M.},
Title = {{A multiplexed microfluidic toolbox for the rapid optimization of
   affinity-driven partition in aqueous two phase systems}},
Journal = {{JOURNAL OF CHROMATOGRAPHY A}},
Year = {{2017}},
Volume = {{1515}},
Pages = {{252-259}},
Month = {{SEP 15}},
Abstract = {{Antibodies and other protein products such as interferons and cytokines
   are biopharmaceuticals of critical importance which, in order to be
   safely administered, have to be thoroughly purified in a cost effective
   and efficient manner. The use of aqueous two-phase extraction (ATPE) is
   a viable option for this purification, but these systems are difficult
   to model and optimization procedures require lengthy and expensive
   screening processes.
   Here, a methodology for the rapid screening of antibody extraction
   conditions using a microfluidic channel-based toolbox is presented. A
   first microfluidic structure allows a simple negative-pressure driven
   rapid screening of up to 8 extraction conditions simultaneously, using
   less than 20 mu L of each phase-forming solution per experiment, while a
   second microfluidic structure allows the integration of multi-step
   extraction protocols based on the results obtained with the first
   device. In this paper, this microfluidic toolbox was used to demonstrate
   the potential of LYTAG fusion proteins used as affinity tags to optimize
   the partitioning of antibodies in ATPE processes, where a maximum
   partition coefficient (K) of 9.2 in a PEG 3350/phosphate system was
   obtained for the antibody extraction in the presence of the LYTAG-Z dual
   ligand. This represents an increase of approx. 3.7 fold when compared
   with the same conditions without the affinity molecule (K=2.5). Overall,
   this miniaturized and versatile approach allowed the rapid optimization
   of molecule partition followed by a proof-of-concept demonstration of an
   integrated back extraction procedure, both of which are critical
   procedures towards obtaining high purity biopharmaceuticals using ATPE.
   (C) 2017 Elsevier B.V. All rights reserved.}},
DOI = {{10.1016/j.chroma.2017.07.094}},
ISSN = {{0021-9673}},
EISSN = {{1873-3778}},
ResearcherID-Numbers = {{Fernandes, Pedro/G-8180-2012
   Bras, Eduardo/I-3337-2019
   Conde, Joao Pedro/F-8533-2012
   Soares, Ruben Rafael Goncalves/H-9580-2019
   Chu, Virginia/I-6048-2014
   Azevedo, Ana Margarida/A-2985-2011
   Aires-Barros, Maria Raquel/E-6021-2010}},
ORCID-Numbers = {{Fernandes, Pedro/0000-0003-0271-7796
   Bras, Eduardo/0000-0001-8928-8431
   Conde, Joao Pedro/0000-0002-5677-3024
   Soares, Ruben Rafael Goncalves/0000-0001-5958-5232
   Chu, Virginia/0000-0002-5306-4409
   Azevedo, Ana Margarida/0000-0001-5659-466X
   Aires-Barros, Maria Raquel/0000-0002-1813-4280}},
Unique-ID = {{ISI:000411536400028}},
}

{"_id":"xmuXFJsXAtqvzhhhE","bibbaseid":"bras-soares-azevedo-fernandes-arevalorodriguez-chu-conde-raquelairesbarros-amultiplexedmicrofluidictoolboxfortherapidoptimizationofaffinitydrivenpartitioninaqueoustwophasesystems-2017","authorIDs":[],"author_short":["Bras, E. J. S.","Soares, R. R. G.","Azevedo, A. M.","Fernandes, P.","Arevalo-Rodriguez, M.","Chu, V.","Conde, J. P.","Raquel Aires-Barros, M."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Bras"],"firstnames":["Eduardo","J.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Soares"],"firstnames":["Ruben","R.","G."],"suffixes":[]},{"propositions":[],"lastnames":["Azevedo"],"firstnames":["Ana","M."],"suffixes":[]},{"propositions":[],"lastnames":["Fernandes"],"firstnames":["Pedro"],"suffixes":[]},{"propositions":[],"lastnames":["Arevalo-Rodriguez"],"firstnames":["Miguel"],"suffixes":[]},{"propositions":[],"lastnames":["Chu"],"firstnames":["Virginia"],"suffixes":[]},{"propositions":[],"lastnames":["Conde"],"firstnames":["Joao","P."],"suffixes":[]},{"propositions":[],"lastnames":["Raquel","Aires-Barros"],"firstnames":["M."],"suffixes":[]}],"title":"A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems","journal":"JOURNAL OF CHROMATOGRAPHY A","year":"2017","volume":"1515","pages":"252-259","month":"SEP 15","abstract":"Antibodies and other protein products such as interferons and cytokines are biopharmaceuticals of critical importance which, in order to be safely administered, have to be thoroughly purified in a cost effective and efficient manner. The use of aqueous two-phase extraction (ATPE) is a viable option for this purification, but these systems are difficult to model and optimization procedures require lengthy and expensive screening processes. Here, a methodology for the rapid screening of antibody extraction conditions using a microfluidic channel-based toolbox is presented. A first microfluidic structure allows a simple negative-pressure driven rapid screening of up to 8 extraction conditions simultaneously, using less than 20 mu L of each phase-forming solution per experiment, while a second microfluidic structure allows the integration of multi-step extraction protocols based on the results obtained with the first device. In this paper, this microfluidic toolbox was used to demonstrate the potential of LYTAG fusion proteins used as affinity tags to optimize the partitioning of antibodies in ATPE processes, where a maximum partition coefficient (K) of 9.2 in a PEG 3350/phosphate system was obtained for the antibody extraction in the presence of the LYTAG-Z dual ligand. This represents an increase of approx. 3.7 fold when compared with the same conditions without the affinity molecule (K=2.5). Overall, this miniaturized and versatile approach allowed the rapid optimization of molecule partition followed by a proof-of-concept demonstration of an integrated back extraction procedure, both of which are critical procedures towards obtaining high purity biopharmaceuticals using ATPE. (C) 2017 Elsevier B.V. All rights reserved.","doi":"10.1016/j.chroma.2017.07.094","issn":"0021-9673","eissn":"1873-3778","researcherid-numbers":"Fernandes, Pedro/G-8180-2012 Bras, Eduardo/I-3337-2019 Conde, Joao Pedro/F-8533-2012 Soares, Ruben Rafael Goncalves/H-9580-2019 Chu, Virginia/I-6048-2014 Azevedo, Ana Margarida/A-2985-2011 Aires-Barros, Maria Raquel/E-6021-2010","orcid-numbers":"Fernandes, Pedro/0000-0003-0271-7796 Bras, Eduardo/0000-0001-8928-8431 Conde, Joao Pedro/0000-0002-5677-3024 Soares, Ruben Rafael Goncalves/0000-0001-5958-5232 Chu, Virginia/0000-0002-5306-4409 Azevedo, Ana Margarida/0000-0001-5659-466X Aires-Barros, Maria Raquel/0000-0002-1813-4280","unique-id":"ISI:000411536400028","bibtex":"@article{ ISI:000411536400028,\nAuthor = {Bras, Eduardo J. S. and Soares, Ruben R. G. and Azevedo, Ana M. and\n Fernandes, Pedro and Arevalo-Rodriguez, Miguel and Chu, Virginia and\n Conde, Joao P. and Raquel Aires-Barros, M.},\nTitle = {{A multiplexed microfluidic toolbox for the rapid optimization of\n affinity-driven partition in aqueous two phase systems}},\nJournal = {{JOURNAL OF CHROMATOGRAPHY A}},\nYear = {{2017}},\nVolume = {{1515}},\nPages = {{252-259}},\nMonth = {{SEP 15}},\nAbstract = {{Antibodies and other protein products such as interferons and cytokines\n are biopharmaceuticals of critical importance which, in order to be\n safely administered, have to be thoroughly purified in a cost effective\n and efficient manner. The use of aqueous two-phase extraction (ATPE) is\n a viable option for this purification, but these systems are difficult\n to model and optimization procedures require lengthy and expensive\n screening processes.\n Here, a methodology for the rapid screening of antibody extraction\n conditions using a microfluidic channel-based toolbox is presented. A\n first microfluidic structure allows a simple negative-pressure driven\n rapid screening of up to 8 extraction conditions simultaneously, using\n less than 20 mu L of each phase-forming solution per experiment, while a\n second microfluidic structure allows the integration of multi-step\n extraction protocols based on the results obtained with the first\n device. In this paper, this microfluidic toolbox was used to demonstrate\n the potential of LYTAG fusion proteins used as affinity tags to optimize\n the partitioning of antibodies in ATPE processes, where a maximum\n partition coefficient (K) of 9.2 in a PEG 3350/phosphate system was\n obtained for the antibody extraction in the presence of the LYTAG-Z dual\n ligand. This represents an increase of approx. 3.7 fold when compared\n with the same conditions without the affinity molecule (K=2.5). Overall,\n this miniaturized and versatile approach allowed the rapid optimization\n of molecule partition followed by a proof-of-concept demonstration of an\n integrated back extraction procedure, both of which are critical\n procedures towards obtaining high purity biopharmaceuticals using ATPE.\n (C) 2017 Elsevier B.V. All rights reserved.}},\nDOI = {{10.1016/j.chroma.2017.07.094}},\nISSN = {{0021-9673}},\nEISSN = {{1873-3778}},\nResearcherID-Numbers = {{Fernandes, Pedro/G-8180-2012\n Bras, Eduardo/I-3337-2019\n Conde, Joao Pedro/F-8533-2012\n Soares, Ruben Rafael Goncalves/H-9580-2019\n Chu, Virginia/I-6048-2014\n Azevedo, Ana Margarida/A-2985-2011\n Aires-Barros, Maria Raquel/E-6021-2010}},\nORCID-Numbers = {{Fernandes, Pedro/0000-0003-0271-7796\n Bras, Eduardo/0000-0001-8928-8431\n Conde, Joao Pedro/0000-0002-5677-3024\n Soares, Ruben Rafael Goncalves/0000-0001-5958-5232\n Chu, Virginia/0000-0002-5306-4409\n Azevedo, Ana Margarida/0000-0001-5659-466X\n Aires-Barros, Maria Raquel/0000-0002-1813-4280}},\nUnique-ID = {{ISI:000411536400028}},\n}\n\n","author_short":["Bras, E. J. S.","Soares, R. R. G.","Azevedo, A. M.","Fernandes, P.","Arevalo-Rodriguez, M.","Chu, V.","Conde, J. P.","Raquel Aires-Barros, M."],"key":"ISI:000411536400028","id":"ISI:000411536400028","bibbaseid":"bras-soares-azevedo-fernandes-arevalorodriguez-chu-conde-raquelairesbarros-amultiplexedmicrofluidictoolboxfortherapidoptimizationofaffinitydrivenpartitioninaqueoustwophasesystems-2017","role":"author","urls":{},"downloads":0},"bibtype":"article","biburl":"http://nanotec.cnr.it/data/nanotec/nanotec-full.bib","creationDate":"2020-04-21T16:04:30.572Z","downloads":0,"keywords":[],"search_terms":["multiplexed","microfluidic","toolbox","rapid","optimization","affinity","driven","partition","aqueous","two","phase","systems","bras","soares","azevedo","fernandes","arevalo-rodriguez","chu","conde","raquel aires-barros"],"title":"A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems","year":2017,"dataSources":["ZTEbWc6bW5f9DTsjF"]}