Precision mapping of the metabolome. Breitling, R., Pitt, A. R, & Barrett, M. P Trends Biotechnol, 24(12):543–548, 2006.
doi  abstract   bibtex   
The global study of the structure and dynamics of metabolic networks has been hindered by a lack of techniques that identify metabolites and their biochemical relationship in complex mixtures. The recent application of Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) to metabolomic analysis suggests a way to tackle the problem. A lower-cost alternative to high-field FTICR-MS, the Orbitrap mass analyzer, promises accelerated activity in this area. Here, we show how the ultra-high mass accuracy and resolution provided by this new generation of mass spectrometers can help to identify metabolites and connect them into metabolic networks. Data from perturbation studies and isotope-tracking experiments can complement this information to create metabolic maps de novo and chart unexplored areas of metabolism.
@Article{breitling06precision,
  author    = {Rainer Breitling and Andrew R Pitt and Michael P Barrett},
  title     = {Precision mapping of the metabolome},
  journal   = {Trends Biotechnol},
  year      = {2006},
  volume    = {24},
  number    = {12},
  pages     = {543--548},
  abstract  = {The global study of the structure and dynamics of metabolic networks has been hindered by a lack of techniques that identify metabolites and their biochemical relationship in complex mixtures. The recent application of Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) to metabolomic analysis suggests a way to tackle the problem. A lower-cost alternative to high-field FTICR-MS, the Orbitrap mass analyzer, promises accelerated activity in this area. Here, we show how the ultra-high mass accuracy and resolution provided by this new generation of mass spectrometers can help to identify metabolites and connect them into metabolic networks. Data from perturbation studies and isotope-tracking experiments can complement this information to create metabolic maps de novo and chart unexplored areas of metabolism.},
  doi       = {10.1016/j.tibtech.2006.10.006},
  file      = {BreitlingEtAl_PrecisionMappingMetabolome_TrendsBiotech_2006.pdf:2006/BreitlingEtAl_PrecisionMappingMetabolome_TrendsBiotech_2006.pdf:PDF},
  keywords  = {metabolite ms},
  owner     = {rasche},
  pmid      = {17064801},
  timestamp = {11.09.2008},
}

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