@ARTICLE{Bresler196479,
author={Bresler, S.E. and Kreneva, R.A. and Kushev, V.V. and Mosevitskií, M.I.},
title={The mechanism of messenger-RNA replication in bacteria},
journal={Journal of Molecular Biology},
year={1964},
volume={8},
number={1},
pages={79-88},
doi={10.1016/S0022-2836(64)80150-9},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-78651163202&doi=10.1016%2fS0022-2836%2864%2980150-9&partnerID=40&md5=2119b6218b51758464d8808249d27e59},
affiliation={Institute of High Molecular Weight Compounds, Academy of Sciences of U.S.S.R., Leningrad},
abstract={The role of both strands of the DNA double helix in the process of information transfer for protein synthesis in the living cell was investigated. For this purpose the transformation of Bacillus subtilis SB 25 his− tryp− by two linked genetic markers under the action of hybrid molecules of DNA was used. The molecular hybrids were obtained by thermal denaturation and annealing of a mixture of two DNAs, each one extracted from a single auxotrophic strain (H25 his− and 168 tryp−). The formation of the heterozygous DNA double helices was proved by special experiments. The transformation procedure excluded DNA synthesis by recipient cells prior to the formation of enzymes for histidine and tryptophan synthesis. It was found that hybrid DNA molecules are able to transform in these conditions the double auxotrophic cells to prototrophic ones. Therefore we must assume that both strands of the DNA double helix can serve as templates for messenger-RNA synthesis. © 1964, Academic Press Inc. (London) Ltd.. All rights reserved.},
author_keywords={M-RNA;  messenger RNA;  salt-glucose medium (see Materials and Methods);  SGM},
correspondence_address1={Bresler, S.E.; Institute of High Molecular Weight Compounds, Academy of Sciences of U.S.S.R., Leningrad},
issn={00222836},
pubmed_id={14149965},
language={English},
abbrev_source_title={J. Mol. Biol.},
document_type={Article},
source={Scopus},
}

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For this purpose the transformation of Bacillus subtilis SB 25 his− tryp− by two linked genetic markers under the action of hybrid molecules of DNA was used. The molecular hybrids were obtained by thermal denaturation and annealing of a mixture of two DNAs, each one extracted from a single auxotrophic strain (H25 his− and 168 tryp−). The formation of the heterozygous DNA double helices was proved by special experiments. The transformation procedure excluded DNA synthesis by recipient cells prior to the formation of enzymes for histidine and tryptophan synthesis. It was found that hybrid DNA molecules are able to transform in these conditions the double auxotrophic cells to prototrophic ones. Therefore we must assume that both strands of the DNA double helix can serve as templates for messenger-RNA synthesis. © 1964, Academic Press Inc. (London) Ltd.. All rights reserved.","author_keywords":"M-RNA; messenger RNA; salt-glucose medium (see Materials and Methods); SGM","correspondence_address1":"Bresler, S.E.; Institute of High Molecular Weight Compounds, Academy of Sciences of U.S.S.R., Leningrad","issn":"00222836","pubmed_id":"14149965","language":"English","abbrev_source_title":"J. Mol. 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The molecular hybrids were obtained by thermal denaturation and annealing of a mixture of two DNAs, each one extracted from a single auxotrophic strain (H25 his− and 168 tryp−). The formation of the heterozygous DNA double helices was proved by special experiments. The transformation procedure excluded DNA synthesis by recipient cells prior to the formation of enzymes for histidine and tryptophan synthesis. It was found that hybrid DNA molecules are able to transform in these conditions the double auxotrophic cells to prototrophic ones. Therefore we must assume that both strands of the DNA double helix can serve as templates for messenger-RNA synthesis. © 1964, Academic Press Inc. (London) Ltd.. 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