Mutations as possible replication errors in bacteria growing under conditions of thymine deficiency. Bresler, S., Mosevitsky, M., & Vyacheslavov, L. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 19(3):281-293, 1973. cited By 23![Mutations as possible replication errors in bacteria growing under conditions of thymine deficiency [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex When Escherichia coli or Bacillus subtilis cells having inhibited thymidylate synthetase activity were incubated for a long time on solid medium supplemented with a limiting concentration of thymine or thymidine (0.1-0.3 μg/ml) most of them became mutants for one or more genetic markers. This "overall mutagenesis" was detected both in Thy- bacteria and in prototrophs for thymine (Thy+) with thymidylate synthetase inhibited by the addition of 5-fluorodeoxyuridine (FUdR) to the growth medium. When thymine (or thymidine) was present in very low amounts (10-3 μg/ml) or was totally absent, the efficiency of mutagenesis decreased some 100-fold. The solid growth medium is essential because it supports the filamentous cells grown under conditions of limiting thymine. For some of the mutants with identified deficiency their ability to revert under the action of different mutagens was studied. Most efficient was 5-bromouracil (BU). This reversion is the characteristic response of mutations due to AT → GC transitions. In addition to single mutants, many multiple mutants were induced. The repair-defective strain of E. coli pol A1- and strains Rec A- and Exr A-, which are also defective in UV-induced mutagenesis, showed a high level of mutation induction under the conditions described. All these results are in accord with the hypothesis that overall low-thymine mutagenesis reflects the accumulation of replication errors in DNA under the conditions of a precursor deficiency. © 1973.
@ARTICLE{Bresler1973281,
author={Bresler, S.E. and Mosevitsky, M.I. and Vyacheslavov, L.G.},
title={Mutations as possible replication errors in bacteria growing under conditions of thymine deficiency},
journal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},
year={1973},
volume={19},
number={3},
pages={281-293},
doi={10.1016/0027-5107(73)90228-5},
note={cited By 23},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0015842943&doi=10.1016%2f0027-5107%2873%2990228-5&partnerID=40&md5=054c0a8e4e43993aad4864f34a38e8c2},
affiliation={Laboratory of Biopolymers, Leningrad Institute of Nuclear Physics, the Academy of Sciences, Birjevoy 6, Leningrad, 199164, Russian Federation},
abstract={When Escherichia coli or Bacillus subtilis cells having inhibited thymidylate synthetase activity were incubated for a long time on solid medium supplemented with a limiting concentration of thymine or thymidine (0.1-0.3 μg/ml) most of them became mutants for one or more genetic markers. This "overall mutagenesis" was detected both in Thy- bacteria and in prototrophs for thymine (Thy+) with thymidylate synthetase inhibited by the addition of 5-fluorodeoxyuridine (FUdR) to the growth medium. When thymine (or thymidine) was present in very low amounts (10-3 μg/ml) or was totally absent, the efficiency of mutagenesis decreased some 100-fold. The solid growth medium is essential because it supports the filamentous cells grown under conditions of limiting thymine. For some of the mutants with identified deficiency their ability to revert under the action of different mutagens was studied. Most efficient was 5-bromouracil (BU). This reversion is the characteristic response of mutations due to AT → GC transitions. In addition to single mutants, many multiple mutants were induced. The repair-defective strain of E. coli pol A1- and strains Rec A- and Exr A-, which are also defective in UV-induced mutagenesis, showed a high level of mutation induction under the conditions described. All these results are in accord with the hypothesis that overall low-thymine mutagenesis reflects the accumulation of replication errors in DNA under the conditions of a precursor deficiency. © 1973.},
correspondence_address1={Bresler, S.E.; Laboratory of Biopolymers, Leningrad Institute of Nuclear Physics, the Academy of Sciences, Birjevoy 6, Leningrad, 199164, Russian Federation},
issn={00275107},
coden={MRFME},
pubmed_id={4201996},
language={English},
abbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},
document_type={Article},
source={Scopus},
}
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This \"overall mutagenesis\" was detected both in Thy- bacteria and in prototrophs for thymine (Thy+) with thymidylate synthetase inhibited by the addition of 5-fluorodeoxyuridine (FUdR) to the growth medium. When thymine (or thymidine) was present in very low amounts (10-3 μg/ml) or was totally absent, the efficiency of mutagenesis decreased some 100-fold. The solid growth medium is essential because it supports the filamentous cells grown under conditions of limiting thymine. For some of the mutants with identified deficiency their ability to revert under the action of different mutagens was studied. Most efficient was 5-bromouracil (BU). This reversion is the characteristic response of mutations due to AT → GC transitions. In addition to single mutants, many multiple mutants were induced. The repair-defective strain of E. coli pol A1- and strains Rec A- and Exr A-, which are also defective in UV-induced mutagenesis, showed a high level of mutation induction under the conditions described. All these results are in accord with the hypothesis that overall low-thymine mutagenesis reflects the accumulation of replication errors in DNA under the conditions of a precursor deficiency. © 1973.","correspondence_address1":"Bresler, S.E.; Laboratory of Biopolymers, Leningrad Institute of Nuclear Physics, the Academy of Sciences, Birjevoy 6, Leningrad, 199164, Russian Federation","issn":"00275107","coden":"MRFME","pubmed_id":"4201996","language":"English","abbrev_source_title":"Mutat. Res. Fundam. Mol. Mech. 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This \"overall mutagenesis\" was detected both in Thy- bacteria and in prototrophs for thymine (Thy+) with thymidylate synthetase inhibited by the addition of 5-fluorodeoxyuridine (FUdR) to the growth medium. When thymine (or thymidine) was present in very low amounts (10-3 μg/ml) or was totally absent, the efficiency of mutagenesis decreased some 100-fold. The solid growth medium is essential because it supports the filamentous cells grown under conditions of limiting thymine. For some of the mutants with identified deficiency their ability to revert under the action of different mutagens was studied. Most efficient was 5-bromouracil (BU). This reversion is the characteristic response of mutations due to AT → GC transitions. In addition to single mutants, many multiple mutants were induced. 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