Measuring and modeling polymer gradients argues that spindle microtubules regulate their own nucleation. Bryan Kayea,b, Olivia Stiehla,b, Peter J. Fostera,b, Michael J. Shelleyd,e, Daniel J. Needlemana,b,c, S. & Fürthauerd Paper Website abstract bibtex Spindles are self-organized microtubule-based structures that segregate chromosomes
during cell division. The mass of the spindle is controlled by the balance between
microtubule turnover and nucleation. The mechanisms that control the spatial regulation
of microtubule nucleation remain poorly understood. Previous work has found that
microtubule nucleators bind to microtubules in the spindle, but it is unclear if this binding
regulates the activity of those nucleators. Here we use a combination of experiments and
mathematical modeling to investigate this issue. We measure the concentration of tubulin
and microtubules in and around the spindle. We found a very sharp decay in microtubules
at the spindle interface, which is inconsistent with the activity of microtubule nucleators
being independent of their association with microtubules and consistent with a model in
which microtubule nucleators are only active when bound to a microtubule. This strongly
argues that the activity of microtubule nucleators is greatly enhanced when bound to
microtubules. Thus, microtubule nucleators are both localized and activated by the
microtubules they generate.
@article{
title = {Measuring and modeling polymer gradients argues that spindle microtubules regulate their own nucleation},
type = {article},
websites = {https://arxiv.org/pdf/1710.08405.pdf},
id = {76d30ed9-17a7-36f3-a6c5-5e9f3b49f427},
created = {2018-04-05T19:19:09.784Z},
accessed = {2018-04-05},
file_attached = {true},
profile_id = {3187ec9d-0fcc-3ba2-91e0-3075df9b18c3},
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last_modified = {2018-04-23T16:00:19.091Z},
read = {false},
starred = {false},
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confirmed = {false},
hidden = {false},
citation_key = {BryanKayeabOliviaStiehlabPeterJ.FosterabMichaelJ.ShelleydeDanielJ.Needlemanabc},
private_publication = {false},
abstract = {Spindles are self-organized microtubule-based structures that segregate chromosomes
during cell division. The mass of the spindle is controlled by the balance between
microtubule turnover and nucleation. The mechanisms that control the spatial regulation
of microtubule nucleation remain poorly understood. Previous work has found that
microtubule nucleators bind to microtubules in the spindle, but it is unclear if this binding
regulates the activity of those nucleators. Here we use a combination of experiments and
mathematical modeling to investigate this issue. We measure the concentration of tubulin
and microtubules in and around the spindle. We found a very sharp decay in microtubules
at the spindle interface, which is inconsistent with the activity of microtubule nucleators
being independent of their association with microtubules and consistent with a model in
which microtubule nucleators are only active when bound to a microtubule. This strongly
argues that the activity of microtubule nucleators is greatly enhanced when bound to
microtubules. Thus, microtubule nucleators are both localized and activated by the
microtubules they generate.},
bibtype = {article},
author = {Bryan Kayea,b, Olivia Stiehla,b, Peter J. Fostera,b, Michael J. Shelleyd,e, Daniel J. Needlemana,b,c, Sebastian and Fürthauerd, undefined}
}
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