Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells. Buchanan, E., Mahony, C., Bam, S., Jaffer, M., Macleod, S., Mangali, A., van der Watt, M., de Wet, S., Theart, R., Jacobs, C., Loos, B., & O'Ryan, C. Scientific Reports, 13:13248, Nature Publishing Group, aug, 2023. ![Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 1 download Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (p \textless 0.01), Feret's diameter and perimeter (p \textless 0.05), and in area2 (p \textless 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p \textless 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p \textless 0.0001), NRF1 (p \textless 0.01), TFAM (p \textless 0.05), STOML2 (p \textless 0.0001), and OPA1 (p \textless 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.
@article{Buchanan2023,
abstract = {Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (p {\textless} 0.01), Feret's diameter and perimeter (p {\textless} 0.05), and in area2 (p {\textless} 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p {\textless} 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p {\textless} 0.0001), NRF1 (p {\textless} 0.01), TFAM (p {\textless} 0.05), STOML2 (p {\textless} 0.0001), and OPA1 (p {\textless} 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.},
author = {Buchanan, Erin and Mahony, Caitlyn and Bam, Sophia and Jaffer, Mohamed and Macleod, Sarah and Mangali, Asandile and van der Watt, Mignon and de Wet, Sholto and Theart, Rensu and Jacobs, Caron and Loos, Ben and O'Ryan, Colleen},
doi = {10.1038/s41598-023-40130-8},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Buchanan et al. - 2023 - Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells.pdf:pdf},
isbn = {0123456789},
issn = {2045-2322},
journal = {Scientific Reports},
keywords = {Cellular imaging,Cellular neuroscience,Gene expression,OA,OA{\_}PMC,fund{\_}not{\_}ack,original},
mendeley-tags = {OA,OA{\_}PMC,fund{\_}not{\_}ack,original},
month = {aug},
pages = {13248},
pmid = {37582965},
publisher = {Nature Publishing Group},
title = {{Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells}},
url = {https://www.nature.com/articles/s41598-023-40130-8},
volume = {13},
year = {2023}
}
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PPA (5 mM) induced a significant decrease in mitochondrial area (p \\textless 0.01), Feret's diameter and perimeter (p \\textless 0.05), and in area2 (p \\textless 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p \\textless 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p \\textless 0.0001), NRF1 (p \\textless 0.01), TFAM (p \\textless 0.05), STOML2 (p \\textless 0.0001), and OPA1 (p \\textless 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. 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