@article{buchanan_propionic_2023,
abstract = {Abstract

Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (
p
{\textless}0.01), Feret's diameter and perimeter (
p
{\textless}0.05), and in area
2
(
p
{\textless}0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (
p
{\textless}0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of
cMYC
(
p
{\textless}0.0001),
NRF1
(
p
{\textless}0.01),
TFAM
(
p
{\textless}0.05),
STOML2
(
p
{\textless}0.0001), and
OPA1
(
p
{\textless}0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.},
author = {Buchanan, Erin and Mahony, Caitlyn and Bam, Sophia and Jaffer, Mohamed and Macleod, Sarah and Mangali, Asandile and {Van Der Watt}, Mignon and {De Wet}, Sholto and Theart, Rensu and Jacobs, Caron and Loos, Ben and O'Ryan, Colleen},
doi = {10.1038/s41598-023-40130-8},
file = {:Users/jacquelinebracher/Zotero/storage/BBGUU6KB/Buchanan et al. - 2023 - Propionic acid induces alterations in mitochondria.pdf:pdf},
issn = {2045-2322},
journal = {Scientific Reports},
month = {aug},
number = {1},
pages = {13248},
title = {{Propionic acid induces alterations in mitochondrial morphology and dynamics in {\{}SH{\}}-{\{}SY5Y{\}} cells}},
url = {https://www.nature.com/articles/s41598-023-40130-8},
volume = {13},
year = {2023}
}

{"_id":"kDTjFquePFMvst9an","bibbaseid":"buchanan-mahony-bam-jaffer-macleod-mangali-vanderwatt-dewet-etal-propionicacidinducesalterationsinmitochondrialmorphologyanddynamicsinshsy5ycells-2023","author_short":["Buchanan, E.","Mahony, C.","Bam, S.","Jaffer, M.","Macleod, S.","Mangali, A.","Van Der Watt, M.","De Wet, S.","Theart, R.","Jacobs, C.","Loos, B.","O'Ryan, C."],"bibdata":{"bibtype":"article","type":"article","abstract":"Abstract Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area ( p \\textless0.01), Feret's diameter and perimeter ( p \\textless0.05), and in area 2 ( p \\textless0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events ( p \\textless0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC ( p \\textless0.0001), NRF1 ( p \\textless0.01), TFAM ( p \\textless0.05), STOML2 ( p \\textless0.0001), and OPA1 ( p \\textless0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.","author":[{"propositions":[],"lastnames":["Buchanan"],"firstnames":["Erin"],"suffixes":[]},{"propositions":[],"lastnames":["Mahony"],"firstnames":["Caitlyn"],"suffixes":[]},{"propositions":[],"lastnames":["Bam"],"firstnames":["Sophia"],"suffixes":[]},{"propositions":[],"lastnames":["Jaffer"],"firstnames":["Mohamed"],"suffixes":[]},{"propositions":[],"lastnames":["Macleod"],"firstnames":["Sarah"],"suffixes":[]},{"propositions":[],"lastnames":["Mangali"],"firstnames":["Asandile"],"suffixes":[]},{"propositions":[],"lastnames":["Van Der Watt"],"firstnames":["Mignon"],"suffixes":[]},{"propositions":[],"lastnames":["De Wet"],"firstnames":["Sholto"],"suffixes":[]},{"propositions":[],"lastnames":["Theart"],"firstnames":["Rensu"],"suffixes":[]},{"propositions":[],"lastnames":["Jacobs"],"firstnames":["Caron"],"suffixes":[]},{"propositions":[],"lastnames":["Loos"],"firstnames":["Ben"],"suffixes":[]},{"propositions":[],"lastnames":["O'Ryan"],"firstnames":["Colleen"],"suffixes":[]}],"doi":"10.1038/s41598-023-40130-8","file":":Users/jacquelinebracher/Zotero/storage/BBGUU6KB/Buchanan et al. - 2023 - Propionic acid induces alterations in mitochondria.pdf:pdf","issn":"2045-2322","journal":"Scientific Reports","month":"aug","number":"1","pages":"13248","title":"Propionic acid induces alterations in mitochondrial morphology and dynamics in \\SH\\-\\SY5Y\\ cells","url":"https://www.nature.com/articles/s41598-023-40130-8","volume":"13","year":"2023","bibtex":"@article{buchanan_propionic_2023,\nabstract = {Abstract\n\nPropionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (\np\n{\\textless}0.01), Feret's diameter and perimeter (\np\n{\\textless}0.05), and in area\n2\n(\np\n{\\textless}0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (\np\n{\\textless}0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of\ncMYC\n(\np\n{\\textless}0.0001),\nNRF1\n(\np\n{\\textless}0.01),\nTFAM\n(\np\n{\\textless}0.05),\nSTOML2\n(\np\n{\\textless}0.0001), and\nOPA1\n(\np\n{\\textless}0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.},\nauthor = {Buchanan, Erin and Mahony, Caitlyn and Bam, Sophia and Jaffer, Mohamed and Macleod, Sarah and Mangali, Asandile and {Van Der Watt}, Mignon and {De Wet}, Sholto and Theart, Rensu and Jacobs, Caron and Loos, Ben and O'Ryan, Colleen},\ndoi = {10.1038/s41598-023-40130-8},\nfile = {:Users/jacquelinebracher/Zotero/storage/BBGUU6KB/Buchanan et al. - 2023 - Propionic acid induces alterations in mitochondria.pdf:pdf},\nissn = {2045-2322},\njournal = {Scientific Reports},\nmonth = {aug},\nnumber = {1},\npages = {13248},\ntitle = {{Propionic acid induces alterations in mitochondrial morphology and dynamics in {\\{}SH{\\}}-{\\{}SY5Y{\\}} cells}},\nurl = {https://www.nature.com/articles/s41598-023-40130-8},\nvolume = {13},\nyear = {2023}\n}\n","author_short":["Buchanan, E.","Mahony, C.","Bam, S.","Jaffer, M.","Macleod, S.","Mangali, A.","Van Der Watt, M.","De Wet, S.","Theart, R.","Jacobs, C.","Loos, B.","O'Ryan, C."],"key":"buchanan_propionic_2023","id":"buchanan_propionic_2023","bibbaseid":"buchanan-mahony-bam-jaffer-macleod-mangali-vanderwatt-dewet-etal-propionicacidinducesalterationsinmitochondrialmorphologyanddynamicsinshsy5ycells-2023","role":"author","urls":{"Paper":"https://www.nature.com/articles/s41598-023-40130-8"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/oGK9wCDkZt6T4SRjs","dataSources":["Krmt6gt9ktB2s6ARh","6STvEtq6QvB8pQwSw","CZ6aN6H7moaiRy3Fg","852h2nyKnzTPoF6Kg"],"keywords":[],"search_terms":["propionic","acid","induces","alterations","mitochondrial","morphology","dynamics","sy5y","cells","buchanan","mahony","bam","jaffer","macleod","mangali","van der watt","de wet","theart","jacobs","loos","o'ryan"],"title":"Propionic acid induces alterations in mitochondrial morphology and dynamics in \\SH\\-\\SY5Y\\ cells","year":2023}