MetExtract: A new software tool for the automated comprehensive extraction of metabolite-derived LC/MS signals in metabolomics research. Bueschl, C., Kluger, B., Berthiller, F., Lirk, G., Winkler, S., Krska, R., & Schuhmacher, R. Bioinformatics, 28(5):736–738, 2012.
doi  abstract   bibtex   
Liquid chromatography-mass spectrometry (LC/MS) is a key technique in metabolomics. Since the efficient assignment of MS signals to true biological metabolites becomes feasible in combination with in vivo stable isotopic labelling, our aim was to provide a new software tool for this purpose.An algorithm and a program (MetExtract) have been developed to search for metabolites in in vivo labelled biological samples. The algorithm makes use of the chromatographic characteristics of the LC/MS data and detects MS peaks fulfilling the criteria of stable isotopic labelling. As a result of all calculations, the algorithm specifies a list of m/z values, the corresponding number of atoms of the labelling element (e.g. carbon) together with retention time and extracted adduct-, fragment- and polymer ions. Its function was evaluated using native (12)C- and uniformly (13)C-labelled standard substances.MetExtract is available free of charge and warranty at http://code.google.com/p/metextract/. Precompiled executables are available for Windows operating systems.rainer.schuhmacher@boku.ac.atSupplementary data are available at Bioinformatics online.
@Article{bueschl12metextract,
  author    = {Christoph Bueschl and Bernhard Kluger and Franz Berthiller and Gerald Lirk and Stephan Winkler and Rudolf Krska and Rainer Schuhmacher},
  title     = {{MetExtract}: A new software tool for the automated comprehensive extraction of metabolite-derived {LC/MS} signals in metabolomics research.},
  journal   = {Bioinformatics},
  year      = {2012},
  volume    = {28},
  number    = {5},
  pages     = {736--738},
  abstract  = {Liquid chromatography-mass spectrometry (LC/MS) is a key technique in metabolomics. Since the efficient assignment of MS signals to true biological metabolites becomes feasible in combination with in vivo stable isotopic labelling, our aim was to provide a new software tool for this purpose.An algorithm and a program (MetExtract) have been developed to search for metabolites in in vivo labelled biological samples. The algorithm makes use of the chromatographic characteristics of the LC/MS data and detects MS peaks fulfilling the criteria of stable isotopic labelling. As a result of all calculations, the algorithm specifies a list of m/z values, the corresponding number of atoms of the labelling element (e.g. carbon) together with retention time and extracted adduct-, fragment- and polymer ions. Its function was evaluated using native (12)C- and uniformly (13)C-labelled standard substances.MetExtract is available free of charge and warranty at http://code.google.com/p/metextract/. Precompiled executables are available for Windows operating systems.rainer.schuhmacher@boku.ac.atSupplementary data are available at Bioinformatics online.},
  doi       = {10.1093/bioinformatics/bts012},
  file      = {BueschelEtAl_MetExtractSoftware_Bioinformatics_2012.pdf:2012/BueschelEtAl_MetExtractSoftware_Bioinformatics_2012.pdf:PDF},
  keywords  = {TrACReview, Metabolic, LC-MS},
  owner     = {fhufsky},
  pmid      = {22238263},
  timestamp = {2012.03.30},
}

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