PLETHORA and WOX5 interaction and subnuclear localisation regulates Arabidopsis root stem cell maintenance. Burkart, R. C., Strotmann, V. I., Kirschner, G. K., Akinci, A., Czempik, L., Maizel, A., Weidtkamp-Peters, S., & Stahl, Y. Technical Report Plant Biology, October, 2019. 00001
PLETHORA and WOX5 interaction and subnuclear localisation regulates Arabidopsis root stem cell maintenance [link]Paper  doi  abstract   bibtex   
Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) that maintains the stemness of the cells in direct contact. The transcription factors WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and the PLETHORAs (PLTs) are both expressed in the SCN where they maintain the QC and regulate the fate of the distal columella stem cells (CSCs). Although WOX5 and PLTs are known as important players in SCN maintenance, much of the necessary regulation of quiescence and division in the Arabidopsis root is not understood on a molecular level. Here, we describe the concerted mutual regulation of the key transcription factors WOX5 and PLTs on a transcriptional and protein interaction level, leading to a confinement of the WOX5 expression domain to the QC cells by negative feedback regulation. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs are necessary for root meristem maintenance as they regulate QC quiescence and CSC fate and show that QC divisions and CSC differentiation correlate. Moreover, we uncover that PLTs, especially PLT3, contains intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that the partitioning of the PLT-WOX5 complexes to NBs, possibly by liquid-liquid phase separation, plays an important role during determination of CSC fate.
@techreport{burkart_plethora_2019,
	type = {preprint},
	title = {{PLETHORA} and {WOX5} interaction and subnuclear localisation regulates {Arabidopsis} root stem cell maintenance},
	url = {http://biorxiv.org/lookup/doi/10.1101/818187},
	abstract = {Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) that maintains the stemness of the cells in direct contact. The transcription factors WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and the PLETHORAs (PLTs) are both expressed in the SCN where they maintain the QC and regulate the fate of the distal columella stem cells (CSCs). Although WOX5 and PLTs are known as important players in SCN maintenance, much of the necessary regulation of quiescence and division in the Arabidopsis root is not understood on a molecular level. Here, we describe the concerted mutual regulation of the key transcription factors WOX5 and PLTs on a transcriptional and protein interaction level, leading to a confinement of the WOX5 expression domain to the QC cells by negative feedback regulation. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs are necessary for root meristem maintenance as they regulate QC quiescence and CSC fate and show that QC divisions and CSC differentiation correlate. Moreover, we uncover that PLTs, especially PLT3, contains intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that the partitioning of the PLT-WOX5 complexes to NBs, possibly by liquid-liquid phase separation, plays an important role during determination of CSC fate.},
	language = {en},
	urldate = {2020-09-25},
	institution = {Plant Biology},
	author = {Burkart, Rebecca C. and Strotmann, Vivien I. and Kirschner, Gwendolyn K. and Akinci, Abdullah and Czempik, Laura and Maizel, Alexis and Weidtkamp-Peters, Stefanie and Stahl, Yvonne},
	month = oct,
	year = {2019},
	doi = {10.1101/818187},
	note = {00001 },
}

Downloads: 0