Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS After Derivatization with Fluorescent Anthranilamide. Butré, C. I., Largy, E., & Delobel, A. Methods in Molecular Biology (Clifton, N.J.), 2271:179–188, 2021.
Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS After Derivatization with Fluorescent Anthranilamide [link]Paper  doi  abstract   bibtex   
N-glycans are described to have a large influence on the properties of therapeutic proteins, including safety and efficacy. For this reason, the extent and type of glycosylation is a characterization parameter for the analysis of antibodies and other therapeutic proteins. The method described here is a fast and high-throughput method for identification and semiquantification of N-glycans by HILIC-FLR-ESI-MS. Sample preparation has been optimized and simultaneous preparation of a large number of samples can be achieved within a day. The use of MS coupled to fluorescence detection is an additional tool for identifying the N-glycan type.
@article{butre_profiling_2021-1,
	title = {Profiling of {N}-{Linked} {Oligosaccharides} of a {Glycoprotein} by {UPLC}-{FLR}-{ESI}-{MS} {After} {Derivatization} with {Fluorescent} {Anthranilamide}},
	volume = {2271},
	copyright = {All rights reserved},
	issn = {1940-6029},
	url = {https://link.springer.com/protocol/10.1007/978-1-0716-1241-5_13},
	doi = {10.1007/978-1-0716-1241-5_13},
	abstract = {N-glycans are described to have a large influence on the properties of therapeutic proteins, including safety and efficacy. For this reason, the extent and type of glycosylation is a characterization parameter for the analysis of antibodies and other therapeutic proteins. The method described here is a fast and high-throughput method for identification and semiquantification of N-glycans by HILIC-FLR-ESI-MS. Sample preparation has been optimized and simultaneous preparation of a large number of samples can be achieved within a day. The use of MS coupled to fluorescence detection is an additional tool for identifying the N-glycan type.},
	language = {eng},
	journal = {Methods in Molecular Biology (Clifton, N.J.)},
	author = {Butré, Claire I. and Largy, Eric and Delobel, Arnaud},
	year = {2021},
	pmid = {33908008},
	keywords = {HILIC, Mass spectrometry, Monoclonal antibodies, N-glycan},
	pages = {179--188},
}
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