Single molecule detection in single living cells. Byassee, T. A., Chan, W. C. W., & Nie, S. In Scanning and Force Microscopies for Biomedical Applications II, volume 3922, pages 2–10, April, 2000. SPIE. ![Single molecule detection in single living cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.
@inproceedings{byassee_single_2000,
title = {Single molecule detection in single living cells},
volume = {3922},
url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/3922/0000/Single-molecule-detection-in-single-living-cells/10.1117/12.383337.full},
doi = {10.1117/12.383337},
abstract = {The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.},
urldate = {2021-11-06},
booktitle = {Scanning and {Force} {Microscopies} for {Biomedical} {Applications} {II}},
publisher = {SPIE},
author = {Byassee, Tyler A. and Chan, Warren C. W. and Nie, Shuming},
month = apr,
year = {2000},
pages = {2--10},
file = {Snapshot:files/2298/12.383337.html:text/html},
}
Downloads: 0
{"_id":"jjrX8crXAFrgi6are","bibbaseid":"byassee-chan-nie-singlemoleculedetectioninsinglelivingcells-2000","author_short":["Byassee, T. A.","Chan, W. C. W.","Nie, S."],"bibdata":{"bibtype":"inproceedings","type":"inproceedings","title":"Single molecule detection in single living cells","volume":"3922","url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/3922/0000/Single-molecule-detection-in-single-living-cells/10.1117/12.383337.full","doi":"10.1117/12.383337","abstract":"The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.","urldate":"2021-11-06","booktitle":"Scanning and Force Microscopies for Biomedical Applications II","publisher":"SPIE","author":[{"propositions":[],"lastnames":["Byassee"],"firstnames":["Tyler","A."],"suffixes":[]},{"propositions":[],"lastnames":["Chan"],"firstnames":["Warren","C.","W."],"suffixes":[]},{"propositions":[],"lastnames":["Nie"],"firstnames":["Shuming"],"suffixes":[]}],"month":"April","year":"2000","pages":"2–10","file":"Snapshot:files/2298/12.383337.html:text/html","bibtex":"@inproceedings{byassee_single_2000,\n\ttitle = {Single molecule detection in single living cells},\n\tvolume = {3922},\n\turl = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/3922/0000/Single-molecule-detection-in-single-living-cells/10.1117/12.383337.full},\n\tdoi = {10.1117/12.383337},\n\tabstract = {The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.},\n\turldate = {2021-11-06},\n\tbooktitle = {Scanning and {Force} {Microscopies} for {Biomedical} {Applications} {II}},\n\tpublisher = {SPIE},\n\tauthor = {Byassee, Tyler A. and Chan, Warren C. W. and Nie, Shuming},\n\tmonth = apr,\n\tyear = {2000},\n\tpages = {2--10},\n\tfile = {Snapshot:files/2298/12.383337.html:text/html},\n}\n\n","author_short":["Byassee, T. A.","Chan, W. C. W.","Nie, S."],"key":"byassee_single_2000","id":"byassee_single_2000","bibbaseid":"byassee-chan-nie-singlemoleculedetectioninsinglelivingcells-2000","role":"author","urls":{"Paper":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/3922/0000/Single-molecule-detection-in-single-living-cells/10.1117/12.383337.full"},"metadata":{"authorlinks":{}},"html":""},"bibtype":"inproceedings","biburl":"https://inbslab.com//wp-content/uploads/2024/03/Chan-2024_03-14.txt","dataSources":["zb3p4i4Fd9wXynbkF","pF7M3AvRzd8m3Nc5t","BqtRhZTYdfYNLhWzp","sBWiDMMhfDHZnN93b","zkBeHFgTXc62hytzj","s3xwaPmQ7EjjEcMSb","5npqWkz6DHBZLyn3J","yc5dXnBwovT8YvCrt","nLduyq7S9B96QB8B5","JiWrSgLBTKbDLqiYi"],"keywords":[],"search_terms":["single","molecule","detection","single","living","cells","byassee","chan","nie"],"title":"Single molecule detection in single living cells","year":2000}